STUDIES ON EFFICACY OF PLANT EXTRACTS IN THE MANAGEMENT OF STEM BORERS ON THREE RICE VARIETIES IN UMUDIKE SOUTHEASTERN NIGERIA

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ABSTRACT

A field trial was undertaken to study the efficacy of plant extracts in the management of rice stem borers at Michael Okpara University of Agriculture, Umudike, Southeastern Nigeria during 2016 and 2017 cropping seasons. The experiment was laid out as 3 x 4 split plot arranged in Randomized Complete Block Design (RCBD) with three replicates. The main plot contained varieties of rice: FARO 44, FARO 52, Ovuku and the sub plot treatments consisted of 5 % each of aqueous extracts of Jatropha curcas L., Ageratum conyzoides L., synthetic insecticide (Furadan 4 G) and the untreated control. Furadan 4 G was applied at 50 g per square metre. The treatments were applied at the tillering stage and booting stage. The results showed that Furadan 4 G and the plant extracts significantly (P<0.05) reduced the percentage dead hearts and whiteheads caused by stem borers compared to untreated control. The yield of the rice in the two years trials were significantly higher (P<0.05) in Furadan 4 G treated plants followed by J. curcas and A. conyzoides than the control. FARO 44 and FARO 52 varieties were less susceptible to the stem borers and had higher grain yield than Ovuku variety. The results suggest that aqueous extracts of J. curcas and A. conyzoides can be suitable alternative to synthetic insecticides for controlling rice stem borers without altering the agro-ecosystem. Incorporating 5 % aqueous extracts of J. curcas and A. conyzoides into lowland rice production in the area will help to mitigate stem borers attack and improve crop yield. FARO 44 and FARO 52 varieties which demonstrated less susceptibility to the stem borers and higher yield should be adopted by farmers to produce bulk of rice.






TABLE OF CONTENTS

Title Page                                                                                                                    i

 

Declaration                                                                                                                 ii

 

Certification                                                                                                               iii

 

Dedication                                                                                                                  iv        

Acknowledgments                                                                                                      v                                                                                                         

Table of Contents                                                                                                       vi

 

List of Tables                                                                                                              ix

                                                                                   

Abstract                                                                                                                      xi                                                                                                                                  

CHAPTER 1: INTRODUCTION                                                                           1

 

1.1       Objective of the Study                                                                                    3

 

CHAPTER 2: LITERATURE REVIEW                                                                4

                                                                                                                     

2.1       Origin of Rice                                                                                                 4

 

2.1.1    History of rice types in Nigeria                                                                      4

 

2.1.2    Geographical distribution of rice                                                                    6

 

2.2       Rainfed Lowland Rice                                                                                                                       14

 

2.3       Morphology of Rice                                                                                       15

 

2.3.1    Vegetative phase                                                                                             15

 

2.4       Reproductive Phase                                                                                       17

 

2.4.1    Nutritional value of rice                                                                                 18

 

2.4.2    Health benefits of rice                                                                                    18

 

2.5       Rice Ecology                                                                                                  19

 

2.6       Recommended Lowland Varieties                                                                 20

 

2.6.1    FARO 44 (SIPI-692033)                                                                                20

2.6.2    FARO 52 WITA 4                                                                                          21

 

2.7       Rice Production Methods                                                                               21

 

2.7.1    Direct-seeded rice                                                                                           22

 

2.7.2    Direct seeding on dry seed bed or dry direct seeded rice                               23

 

2.8       Insect Pests in Rice Production                                                                      23

 

2.8.1    Stem borers of rice and their economic importance                                      25

 

2.8.2     Chilo zacconius Bleszynski, African striped rice borer

(Lepidoptera: Pyralidae)                                                                                 26

 

2.8.3     Maliarpha separatella Ragonot , African white borer

(Lepidoptera: Pyralidae)                                                                                 27

 

2.8.4     Sesamia calamistis Hampson, African pink borers

(Lepidoptera: Noctuidae)                                                                               28

 

2.8.5     Diopsis longicornis Macquart, Stalk-eyed fly (Diptera: Diopsidae)                   29

 

2.9       Management of Rice Stem Borer                                                                   30

 

2.10     Chemical Insecticides for the Management of Rice Stem Borer                        32

 

2.11      Botanical Products for the Management of Rice Stem Borer                                37

 

CHAPTER 3: MATERIALS AND METHODS                                                    45

 

3.1       Experimental Site                                                                                           45

 

3.2       Soil Sampling and Analysis                                                                           47

 

3.3       Planting Materials                                                                                          48

3.4       Field Preparation and Experimental Design                                                 48

3.5       Application of Treatments                                                                              49

 

3.5.1    Preparation of aqueous extract of J. curcas                                                   49

 

3.5.2    Preparation of aqueous extract of A. conyzoides                                            49

 

3.6       Data Collected                                                                                                50

 

3.7       Data Analysis                                                                                                  51

 

CHAPTER 4: RESULTS AND DISCUSSION                                                      52

 

4.1       Results                                                                                                            52

 

4.1.1    Efficacy of plant extracts and furadan 4 G on mean percentage dead

heart 55 DAP in 2016 and 2017 planting seasons.                                         52

 

4.1.2    Efficacy of plant extracts and furadan 4 G on mean percentage dead

heart 70 DAP in 2016 and 2017 planting seasons.                                         54 

  

4.1.3    Efficacy of plant extracts and furadan 4 G on mean percentage

whiteheads during 2016 and 2017 planting seasons.                                      57       

 

4.1.4    Efficacy of plant extracts and furadan 4 G on mean number of larvae per

            rice stem during 2016 and 2017 planting seasons.                                         59

 

4.1.5    Efficacy of plant extracts and furadan 4 G on mean number of panicle per

            rice plant during 2016 and 2017 planting seasons.                                         61

                                      

4.1.6    Efficacy of plant extracts and furadan 4 G on mean number of tillers

per plant stem 55 DAP during 2016 and 2017 planting seasons.                 63

 

4.1.7    Efficacy of plant extracts and furadan 4 G on mean number of

tillers per rice plant 70 DAP during 2016 and 2017 planting seasons.           65

 

4.1.8    Efficacy of plant extracts and furadan 4 G on mean grain yield of

rice varieties during 2016 and 2017 planting seasons.                                   67

 

4.2       Discussion                                                                                                       69

 

4.2.1    Efficacy of aqueous plant extracts and furadan 4 G in the management

of rice stem borers                                                                                          69

 

4.2.2    Efficacy of plant extracts and furadan 4 G on dead heart and

whiteheads of lowland rice                                                                             72

 

4.2.3    Efficacy of plant extracts and furadan 4 G on yield of lowland rice                        75                                                                                                                                                       

 

CHAPTER 5: CONCLUSION AND RECOMMENDATIONS                           78

 

5.1       Conclusion                                                                                                      78

5.2       Recommendations                                                                                          78

           

            REFERENCE                                                                                                 80

           





 

 

LIST OF TABLES

Rice growing systems in the North-east zone                                                                  8

Rice growing systems in the North-west zone                                                          9

Rice growing systems in the South-center zone                                                                                             10                                                                                                      

Rice growing systems in the South-east zone                                                                                                11                                                                                                      

Rice growing systems in the South-south zone                                                                                              12                                           

Rice growing systems in the South-west zone                                                                                               13                                           

Agrometerological data of the experimental site for

2016 and 2017                                                                                                                                                46

 

Soil physico-chemical properties of the experimental sites in

2016 and 2017 cropping seasons                                                                                47

  

   Efficacy of aqueous extracts and furadan 4 G against rice stem

   borer on lowland varieties of rice during 2016 and 2017 planting

   seasons assessed from dead heart (% DH) 55 DAP                                                              53

 

Efficacy of aqueous extracts and furadan 4 G against rice stem borer

on lowland varieties of rice during 2016 and 2017 planting seasons

assessed from dead heart (% DH) 70 DAP                                                                         56

 

Efficacy of aqueous extracts and furadan 4 G on reduction of

whiteheads (% WH) 85 DAP in lowland varieties of rice during 2016

and 2017 planting seasons.                                                                                                    58

 

Efficacy of aqueous plant extracts and furadan 4 G on reduction of

larvae number per rice stem in lowland varieties of rice during 2016

and 2017 planting seasons.                                                                                                 60                   

Efficacy of aqueous plant extracts and furadan 4 G on the number

of panicles per stem on lowland varieties of rice during the 2016

and 2017 planting seasons.                                                                                                 62

 

Efficacy of aqueous plant extracts and furadan 4 G on number of

tillers per rice plant 55 DAP during the 2016 and 2017 planting seasons                        64

 

 

Efficacy of aqueous plant extracts and furadan 4 G on number of

tillers per rice plant 70 DAP during the 2016 and 2017 planting seasons                        66

 

Efficacy of aqueous plant extracts and furadan 4 G on lowland varieties

of rice against rice stem borers and effects on yield in tonnes per hectare

during the 2016 and 2017 planting seasons                                                                        68

 





 

 

 

 

CHAPTER 1

INTRODUCTION

Rice (Oryza sativa L.) is one of the major diet of over half of the world’s population. Globally, production of rice is ranked third after wheat and maize while in Nigeria is ranked sixth after sorghum, millet, cowpea, cassava and yam (Bandyopadhay and Roy, 1992; Dauda and Dzivama, 2004; Olaleye et al., 2004). It is grown in all agro ecological zones in Nigeria. Nigeria is the largest producer of rice in West Africa with average of 3.2 mn tonnes of paddy rice (USDA, 2002, Nwilene et al., 2008).  The global rice production is estimated at 4.5 mn tonnnes annually which has an average yield of 4.25 mn stonnes per hectare (Fazlollah et al., 2011). In Nigeria land under rice cultivation and production is increasing every year, from 1.61 mn hectares in 2005/2006 to 2.0 mn hectares in 2009/2010 and production also moved from 3.3 mn kgha-1 in 2005/2006 to 4.1 mn kgha-1 in 2009/2010. Currently, rice area and production have increased to 2.5 mn hectares and 2.7 mn tonnes in 2016/2017 (Ibrahim et al., 2011). An average Nigerian consumes about 24.8 kg of rice per year (Uche et al. 2016).

Although, rice is grown virtually in all agroecological zones of Nigeria, rice production is still far below the potentials and domestic needs. One of the major constraint for the low yield of rice in Nigeria is attacked by insect pests (Imolehin and Ukwungwu, 1992). Rice plant is attacked by a large number of insect pests in West Africa (Heinrichs and Barrion, 2004). Among all the insect pests of rice, stem borers have been reported to cause greater damage to rice plants. There are about 20 species of stem borers identified which are destructive to rice plant worldwide. However, only four species are of economic importance in Nigeria. These include Chilo zacconius Bles, Diopsis macropathalman Daman, Maliarpha separatella Rog, and Sesamia calamistis Hampson (Heinrichs and Barrion, 2004). The spreading and copiousness of these species vary among rice ecosystems within a given location. For example; M. separatella and Chilo spp occur in all climatic zones in West Africa but they are more abundant in the rain fed lowland and irrigated ecosystems than in the uplands and are the most abundant stem borer species in the mangrove swamps while Sesamia spp prevail in the uplands (Heinrichs and Barrion, 2004).

These borers attack rice plant at all growth stages from vegetative stages to reproductive stage. In the vegetative stage of the rice plant, stem borers’ larvae bore at the base and feed on the leaf sheath initiating broad longitudinal whitish areas at the feeding sites which prevents the central leaf whorls from unfolding, resulting in brownish colour and death, leading to condition known as “dead hearts”. Damaged shoot do not produce a panicle and thus produce no grain.  During the reproductive stage of the rice plant (panicle initiation to milk grain), the stem borers’ bore through the upper nodes and feed toward the base causing serious damage on the developing panicle at its base. As a result, the panicle is unfilled and whitish in colour; the condition known as ‘whitehead’ (WH) rather than filled with grain and brownish colour (Amaugo and Emosairue, 2003; Mahmood-ur-Rehman et al., 2007). Report has shown that rice stem borers cause yield losses ranging from 30 to 80 %. A loss of 100 % has been recorded in worst affected fields in Nigeria (Imolehin and Ukwungwu, 1992). In many parts of Africa the borers destroy 30 % -50 % of plant tillers during the wet cropping season thereby compromising the whole harvest (Dakaouo et al., 1991).

In the past emphasis has been on the use of synthetic insecticides for the control of stem borers and successful control of it has been achieved through the use of a number of conventional insecticides (Hill and Waller, 1988; Amaugo and Emosairue, 2003). However, the indiscriminate use of insecticides has caused a number of adverse side effects such as the emergence of resistant species of insects, environmental pollution and hazards to farmers (Hassall, 1990). In addition, insecticides are known to undermine the ecosystem for sustainable production of rice and are generally not affordable to farmers. In order to relieve growing public fears regarding the effects of synthetic insecticides on human health and environmental impact much attention has been given to botanical pesticides in the recent decades. Botanicals are considered environmentally friendly; besides, this method does not only reduce application of synthetic insecticides, but also reduce the cost of pest management, which is an important factor for farmers in developing countries. Furthermore, in recent years there has been an increased interest in the use of biopesticides particularly in cropping system where the use of natural enemies are being emphasized as a major component of integrated pest management (Rausell et al., 2000). Use of these natural compounds in place of synthetic insecticides can reduce environmental pollution, preserve non-target organisms and prevent insecticide induced pest resurgence.

Bioinsecticides are rarely as effective as chemicals in their crude extract form. The efficacies of botanicals are largely demonstrated in insect management and have been encouraged for use by resource poor farmers (Huang et al., 2000; Dal Bello et al., 2001; Taponjou et al., 2002). Plants produce a diversity of biologically active substances that affect the growth and development of other organisms and can provide protection against the herbivores. These plant products discourage or prevent an attack from the non-adapted organisms and play an important role in the ecology and physiology of phytophagous insects (Sukumar, 1993). Shukle et al. (1992) evaluated the field efficacy of 3 % neem oil solution and 5 % neem seed kernel extract and reported a significant reduction in the populations of the green leaf hopper, the white backed plant hopper and the leaf folder in treated plots of rice when compared with the untreated plots. Amaugo and Emosaire, (2003) evaluated aqueous and acetone extracts of seed kernels of neem (Azadirachta indica A. Juss); nutmeg (Monodora myristica (Gaertn.) Dunal; physic nut (Jatropha curcas. L.); castor-oil (Ricinus communis L.); synthetic insecticide (Monocrotophos) and the untreated check to control rice stem borer and reported that neem seed and physic nut seed kernel extracts were statistically better than other plant extracts in controlling stem borers and influencing yields of the crop. This research evaluated the field effectiveness and performance of two indigenous medicinal plant extracts (J. curcas and Ageratum conyzoides) for the control of lowland rice stem borers and the impact on the yield of different varieties of rice crop.


1.1       OBJECTIVES OF THE STUDY

The following are the objectives of this research:

      I.         to evaluate the efficacy of aqueous J. curcas and A. conyzoides in the management of rice stem borer and yield of rice.

    II.         to determine suitable rice variety in this agroecological zone



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