ABSTRACT
The stream water was examined for
microorganism present in it. The isolated and identified bacteria are Bacillus cereus and Staphylococcus
aureus. While fungi are Aspergilllus niger
and Sacchromyces cerevisae Pnicillium chrysogenum.
TABLE
OF CONTENT
TITLE
PAGE I
CERTIFICATION
II
DEDICATION III
ACKNOWLEDGMENT IV
TABLE
OF CONTENT V
ABSTRACT
VI
CHAPTER
ONE
1.0 INTRODUCTION 1
– 2
CHAPTER
TWO
2.0 LITERATURE REVIEW 3
– 6
2.1 PURPOSE OF STUDY 6
CHAPTER
THREE
3.0 MATERIALS
AND METHODS 7
3.1 STERILIZATION
OF MATERIALS 7
3.2 COLLECTION
OF SAMPLE 7
3.3 PREPARATION
OF MEDIA 7
– 8
3.4 SERIAL
DILUTION 8
3.5 INOCULATION
OF AGAR 8
3.6 SUB-CULTURING
OF MICROBIAL GROWTH 8
3.7 MAINTENANCE
OF PURE CULTURE 8
3.8 CHARACTERIZATION
OF FUNGAL ISOLATES 9
3.9 CHARACTERIZATION
OF BACTERIAL ISOLATES 9
3.9.0 GRAM
STAINING 9
3.9.1 SPORE
STAINING 10
3.9.2 COAGULASE
TEST 10
3.9.3 OXIDASE
TEST 10
3.9.4 CATALASE
TEST 10
3.9.5 MOTILITY
TEST 10
– 11
3.9.6 IDENTIFICATION
OF FUNGAL ISOLATES 11
3.9.7 IDENTIFICATION
OF BACTERIAL ISOLATES 11
CHAPTER FOUR
4.0 RESULTS
AND DISCUSSION 12
– 15
CHAPTER FIVE
5.0 CONCLUSION
AND RECOMMENDATION 16
– 17
REFERENCES 18
CHAPTER ONE
1.0 INTRODUCTION
Water is the one of the most common
substances known and it is good solvent for many substances, water occur at
room temperature as clear, colourless, tasteless liquid which freezing into ice
at zero degree centigrade and boil at 1000c. it is essential for
life on earth.
According to World Health Organization
(WHO) in 1967 is the most important need of living things, it is second only to
the air we breath, in fact some scientist believe that all life began in water.
Also water is a molecule that consist of two hydrogen atoms and one oxygen
atom, when these atoms are heated to a high temperature they join to for water.
It occurs in three natural states solid, liquid and gaseous states. Water and
ice covered about 75% of the earth surface and water vapour is an important constituent
of the atmosphere (Ababio 1985) It exists under major two types which include:.
Surface
Water: They include the
streams, lakes, and shallow wells. The air through which the rain passes may
contaminate the water.
Ground
Water: They originate
from deep well and subterraneous springs. This is virtually free of bacteria
due to filtering action of soil deep sand and rock. However, it may be come
contaminated when it flows along the channels.
Water in Microbiology can be
contaminated when it contains a chemical or biological poison or an infectious
agent. These condition also apply to water which is polluted except that the
agent or poison is often obvious and water carries an unpleasant taste or
appearance. (Encyclopedia America
1988).
Portability refers to the drinkability
of water when it is fit for drinking, when unportable it is unfit for
consumption due to some contaminant or pollutant (Earnet 1974) other sources of
contamination are various types of establishments and agricultural farm etc. Possible
sources of microbial contamination of a body of water are soil and agricultural
run-off, farm animals, industrial waste, discharges from sewage treatment
plants and storm water run-off from urban areas.
Contamination by sewage or human
excrement and pollution by animals causes the greatest danger associated with
drinking water in most developing countries. So it is necessary to examine the
quality of water before consumption.
Monitoring and detection of indicator
and disease causing microorganisms are a major part of sanitary microbiology.
Bacteria from the intestinal tract generally do not survive in the aquatic
environment. They are under physiological stress and gradually lose their
ability to form colonies on differential and selective media (Prescott 7th Edition).
Click “DOWNLOAD NOW” below to get the complete Projects
FOR QUICK HELP CHAT WITH US NOW!
+(234) 0814 780 1594
Buyers has the right to create
dispute within seven (7) days of purchase for 100% refund request when
you experience issue with the file received.
Dispute can only be created when
you receive a corrupt file, a wrong file or irregularities in the table of
contents and content of the file you received.
ProjectShelve.com shall either
provide the appropriate file within 48hrs or
send refund excluding your bank transaction charges. Term and
Conditions are applied.
Buyers are expected to confirm
that the material you are paying for is available on our website
ProjectShelve.com and you have selected the right material, you have also gone
through the preliminary pages and it interests you before payment. DO NOT MAKE
BANK PAYMENT IF YOUR TOPIC IS NOT ON THE WEBSITE.
In case of payment for a
material not available on ProjectShelve.com, the management of
ProjectShelve.com has the right to keep your money until you send a topic that
is available on our website within 48 hours.
You cannot change topic after
receiving material of the topic you ordered and paid for.
Login To Comment