antibacterial activity of three type of medicate soap on staphylococcus aureus
isolated from wound infections was conducted. Fifty individual with would
infection within the age range of 9-73 years were sampled. Swabsticks were used
to collect specimens from wound infection. each swabstick was streaked
separately over plates of nutrient and macconkey agar and later incubated at 370c.
out of the fifty (50) individuals sampled 25(50%) were mostly infected with
staphylococcus aureus followed by pseudomonas acruginqu (22%) staphylococcus epidermis (10%) least by
Exchericha coli b(12%) The organisms occur within the age range 9-13 years with
8 (16%) followed by 14-18 years 4(8%) next by the age range of 19-23 years
3(6%) followed by 24-28 years with 2(4%) followed 24-28 years with 2(4%) and
the heart in the age range are 29-33,34-38,39-43,44-48,49-53,54-58 64-68 with
1(2%) each while there was no isolation in the age range 59-63. The study also
revealed that all the staphylococcus aureus isolated were sensitive to the
three medicated soap. The means of inhibition was highest in Aleppo medicated
soap with 12.92 mm followed by temperate medicated soap with 11.2mm and least
in carat medicated soap with 10.56 mm. Also the staphylococcus aureus isolated
were also sensitive to the commercial autibutics used as couplers the means
zones of inhibition was highest in ampiclox with 12.8 mm followed by gentamyain
with 12.04 mm and the heart in penicillin with 10.4 mm. there was a close
relationship in the zenes of inhibition between the three medicated soap and
the commercial antibiotics used as coconutrels. The result showned that
medicated soaps were effective against staphylococcus aureus involved in would
infection in humans and are comparable
to commercial antibiotics. Therefore medicated soap can be used in cleaning the
skin particularly in the affected part of the skin during the time one has hurt
or cut on the skin.
TABLE OF CONTENTS
Aims and objectives
Statement of the problem
Justification of the study
Limitation Of The Study
Antibacterial activity of soap
Medical relevance of antimicrobial soap
Normal skin microbiota and soap
Material and methods
Collection of soap sample
Preparation of culture media
Collection of bacterial organisms from would infection
Microbial count, grams stain and microscopic
Biochemical test for identification of isolates
LIST OF TABLE
Table 1: Age distribution number sampled as well as
percentages with staphylococcus aureus and other organisms
Table 2: Age
distribution as well as percentage of each isolated organisms
Table 3: Preliminary
identification of bacterial isolates
Table 4 : Biochemical
test carried out for identification of bacterial isolates
Table 5: Sensitivity
test using three medicated soap and three antibiotics indicating their zones of
to pelage et al (1986) antibacterial activity is the ability to either destroy
bacteria or inhibit their growth. This is significant with respects to the
human body in preventing sepsis and skin infections. Also Derland (1981) states
that medicated soap have the germicidal substance like chloroxyhlenol potassium
mercuric iodide, trichlorocarbanlide etc. incorporated into them, in order to
enhance their antibacterial activity. These germicide substance are normally added in a specified
amount and percentage of the substance used are always stated on the soapcase
or inside the lesflet which certains the
information on how to use the soap for various purposes.
states that soap may be defined as a
chemical compound resulting from the interaction of fathy acids oil and caustic
soda (alkali) possesing the characteristic soap like properties of detergents,
surface tension lowering wetting and emulsifying power and gel formation. All
fatty oils and fats. Are mixtures of glycoside compounds (erters) of trihydric
alcohol, glycosides and some fatty acids
such as plasmatic acid the chemistry of soap manufacturing may be expressed the following equation which is
know as saponification (Anon (1964).
+ Na 0H Naoco15H31 (zH31 + c3H5(oH)3
sodium palurtate glycerion
further explain that a bactericidal soap cernirts existentially of the following
0.1 to 3% weight based on the total weight of the soap.
0 to 10% by
weight based on the total weight of the soap in a compound of formular
Here R represents an alkyl or
alkenye group of 8 to carbon atoms.
to Johnson (1978) antibacterial soap may
include 2,4,4,trichloro 2,2 hydroxydiphenyl in an amount 0.05 to 5% by weight
and at least one phosphorus oxyacid. Phosphorus oxyacid salt phosphoric ester
is represented by
in (8-20 alkyl, R2 is hydrogen or methyl and is (1-10). R1 is the
same group as R2 or H alkali metal and n is H or alkali metal. Such
a soap has a wide range of antibacterial activities and marked resistance of
discoloration upon exposure to sunlight
(Kaw,1981) another invention relates to an autimicrobial composition consisting
of ammoniated zinc sulphate optionally formulated into sufficient formulation
and toilet bars. Medicated soap incorporate in their composition
germicidal agent which include
hexachlorophene mercuric which trichoran, trichlorocarbaucide .
(1958) described sterilization as the complete destruction of all living
matters. In medical sense, it is often used in a restricted sense to refer
to the destruction of pathogenic
to William (1979) wound is defined as disruption of cellular and anatomic
continuity while its healing is the restoration of continuity. That biological procen can only be
accomplished by regeneration, cell proliferation and collage production which
can be alleviated washing the would surface especially with medicated soap
which due to its concert of pherolic compound help in keeping off organisms
like staphylococcus aureus escherichia coli and Pseudomonas aeruginosa always
from the would to a certain stage. Wound can also result when the operative
barrier of the skin is breached by
traumatic invasion or whether it is caused by trauma or internationally by
surgery. The open area is susceptible to microbial invasion and once a would
has become infected pus form in the injured area resulting to wound abbess.
Baker et al
decried antisepsis as the most convenient way of preventing infection usually
by inhibiting the growth of bacteria. And most disinfectant when suitably diluted have the artixeptic action.
Different method are employed for the destruction of bacteria or for getting
rid of them and those method can be
conveniently divided into chemical physical and mechanical methods.
Baker et al
(1985) explained that chemical agent function as a sterilizing agent by the
following lethal mechanisms
Disruption of the cell membranes
orgymatic systems of the organisms emzyme poison)
Co-aqulation of protein
OBJECTIVES OF THE STUDY
To isolate staphylococcus aureus from wound infection
To determine the artibacterial activity of three
medicated soap on staphylococcus aureus isolated from wound infection.
Dorland (1981) stated that medicated
soaps have germicidal substances like chloroxylenol pothaium, mercuric
widide trichlorocarberihide etc
incorporated into them in order to greatly their antibacterial activity. It is
therefore necessary to investigate the artibaterial activity of three medicated
soap (Asepos carat and temperate) on staphylococcus aureus isolated from wound
soap have antibacterial activity
soaps have no artibaterial activity.
OF THE STUDY
of the project work will indicate the antibacterial activity of medicated soap.
And if favourable, then medicated soap can be used for washing the surface of
manor wound before the application of further medication
OF THE STUDY
is limited to three types of medicated soaps which are:
Asepso medicated soap
Carat medicated soap
Tempovate medicated soap
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