ABSTRACT
This study evaluated the prevalence of air microflora on Nigerian currency notes which were randomly collected from individuals within Umudike. A total of 10 pieces of Naira notes (2 pieces of each of the denominations of N10, N20, N50, N100, and N200) were randomly collected from individuals within Umudike. Samples were collected in sterile leather bags using disposable sterile hand gloves. These were immediately taken to the laboratory for analysis. A total of five (5) bacteria and two(2) fungal strains were obtained. The details of these isolates include; Bacillus sp, Staphylococcus aureus, Pseudomonas sp, Escherichia coli, and klebsiella sp, Aspergillus flavus, and Rhizopus stolonifer. The total heterotrophic plate count ranged from 3.2 × 105cfu/ml to 6.6 × 105 cfu/ml with the 10 naira note giving the highest mean count of 6.6 × 105 cfu/ml whereas the 200 naira note had the lowest mean count of 3.2 × 105cfu/ml. The coliform plate count ranged from 3.9 × 105cfu/ml to 6.2 × 105cfu/ml with the 100 naira note giving the highest mean count of 6.2 × 105cfu/ml whereas the 50 naira note had the lowest mean count of 3.9 × 105cfu/ml. The total fungal plate count ranged from 3.1 × 105cfu/ml to 4.8 × 105cfu/ml with the 50 naira note currency giving the highest mean count of 4.8 × 105cfu/ml whereas the 20 naira note currency had the lowest mean count of 3.1 × 105cfu/ml. It was observed that Staphylococcus aureus is the most frequently occurring bacterial isolates from the naira notes with a percentage occurrence of (33.3%), followed by Bacillus sp with a percentage occurrence of (26.7%), then Escherichia coli with a percentage occurrence of (20.0%) and Klebsiella sp with a percentage occurrence of (13.0%), whereas Pseudomonas aeruginosa has the least percentage occurrence of (6.7%). In that same sequence, Aspergillus flavus is the most frequently occurring fungal isolates from the naira notes with a percentage occurrence of (75.0%) whereas Rhizopus stolonizer has the least percentage occurrence of (25.0%). From the result of the present study, The likelihood of contacting infections due to contact with naira notes is high considering the microbial count gotten in the study. It can be recommended that people should improve their personal health consciousness by washing hands after handling of money notes and avoiding using saliva during counting of paper money notes.
TABLE OF CONTENTS
Title
Page i
Certification iii
Dedication iv
Acknowledgement v
Table
of Contents vi
List
of Tables ix
Abstract x
CHAPTER ONE
1.0 Introduction 1
1.1 Aims and Objectives 2
CHAPTER TWO
2.0 Literature Review 3
2.1 The Nigerian Currency 3
2.2 Air Microflora 3
2.3 Micro Organisms Associated with Airborne
Contaminations 4
2.3.1 Bacteria Example 4
2.3.2 Fungi Example 5
2.3.3 Virus Example 5
2.4 Factors That Affect the Air Microflora 5
2.4.1 Temperature 5
2.4.2 Relative
Humidity 5
2.4.3 Microbes 6
2.4.4 Chemicals 7
2.4.5 Radiation 7
2.4.6 Oxygen,
OAFs, and Ions 8
2.5 Bioaerosol Control 8
2.5.1 Ventilation 9
2.5.2 Filtration 9
2.5.3 Biocidal Control 10
CHAPTER THREE
3.0 Materials and Method 11
3.1 Study Area 11
3.2 Collection of Samples 11
3.3 Sterilization of Materials 11
3.4 Preparation of Culture Media 11
3.5 Preparation of Sample and Inoculation of
Samples 12
3.6 Purification
of Isolates 12
3.7 Identification of Bacterial Isolates 12
3.7.1 Gram
Staining 13
3.7.2 Biochemical Test 13
3.7.2.1 Indole test 13
3.7.2.2 Methyl red (MR) 14
3.7.2.3 Voges proskauer (VP) 14
3.7.2.4 Hydrogen sulphide test (H2S) 14
3.7.2.5 Citrate test 14
3.7.2.6 Urease test 15
3.7.2.7 Catalase test 15
3.7.2.8 Coagulase test 15
3.7.2.9 Sugar fermentation test 15
3.7.2.10
Starch test 16
3.8 Identification of Fungal Isolates 16
CHAPTER FOUR
4.0 Results 17
CHAPTER FIVE
5.0 Discussion, Conclusion and Recommendation 34
5.1 Discussion 34
5.2 Conclusion 38
5.3 Recommendation 38
References 39
LIST
OF TABLES
TABLE
|
TITLE
|
PAGE
|
1
|
Total viable microbial count of naira notes samples
|
20
|
2
|
Morphological
Characteristics of Bacterial Isolates
|
21
|
3
|
Biochemical
Identification, Gram Reaction and Sugar Utilization Profile of bacterial
isolates from the naira notes samples
|
22
|
4
|
Cultural Morphology and
Microscopic Characteristics of the Fungal Isolates from the naira notes samples
|
23
|
5
|
Percentage Occurrence of the
Bacterial isolates from naira notes
samples
|
24
|
6
|
Percentage Occurrence of the
fungal isolates from naira notes
samples
|
25
|
CHAPTER ONE
1.0 INTRODUCTION
The Naira note is the official currency of
the Federal Republic of Nigeria, issued and regulated by the Central Bank of
Nigeria (CBN). According to CBN, the expected lifespan of the Naira notes is 24
months but the mishandling reduces this to less than 6 months. The abused Naira
note denotes the currency, which had been fairly long (not more than 24 months)
in circulation, mishandled, structurally disfigured, literally mutilated and
for most of the time they are dirty. Incidentally, abused Naira notes were
reported as vehicles of bacterial, mold and other parasitic infections and
agents of cross contamination (Awodi et
al., 2001).
Paper and polymer currency notes are
extensively used in barter and trade (exchange for goods and services),
settlement of debts and for deferred payments in economic activities. Daily
transactions have made the naira note to pass through many hands and pathogens
become imposed on them before they are finally deposited in banks (Awodi et
al., 2000). In Nigeria, the naira notes presently in circulation are abused
by the different ways they are handled and stored which may include but not
limited to squeezing, spraying, stapling, cello-taping, keeping naira notes in
brassiere, socks and pockets, under the carpet or rugs, writings on them etc.
However, there is well documented evidence suggesting that currency notes could
act as fomites with enormous potential to carry microbes. The contamination of
the naira notes could be from several sources, it could be from the atmosphere,
during storage, usage, handling or production (Ogba, 2007). The contaminated
currency notes go in circulation and contaminate the hands of others and across
borders transmitting microorganisms in the process since money is not screened
for microbes (Pope et al., 2002). Most of these organisms are pathogenic
while others are normal flora of the human skin; however, some e.g. S.
aureus and P. aeroginosa can be opportunistic pathogens. This
suggests that the notes could serve as formites for some infectious agents.
These routes of transmission are of great importance in the health of many
populations in developing countries, where the frequency of infection is a
general indication of local hygiene and environmental sanitation levels.
The
survival of various microorganisms on money and other fomites, with their
transmission via the hands of market men and women and other users is often
overlooked as enteric disease reservoir (Michaels, 2002). Pathogenic
microorganisms that may survive on currency notes may serve as a potential
source of enteropathogens (Michaels, 2002; Cardoen et al., 2009;
Lamichhane et al., 2009). Contamination of objects by pathogenic
microorganisms is of much public health concern as contaminated meat, butcher’s
hands, utensils, tables and the exchange of currency can be sources of
transmitting pathogens. In addition, currency notes have also been evaluated
for their potential to transmit infectious pathogens like Ascaris
lumbricoides, Enterobius vermicularis, Trichuris trichiura and
Taenia species. Carrier micro-organisms apart from reducing the lifespan
of the notes, have been documented to cause infections in the skin, eye,
gastrointestinal tract, internal organs (Yildiran et al., 2006), as well
as the respiratory tract (Denning, 2006) in humans. Microorganisms such as Micrococcus
spp., Corynebacterium spp., Vibrio cholerae, Mycobacterium
tuberculosis and members of the Enterobacteriacea family top the
list subsequently.
1.1 AIMS AND OBJECTIVES
The
aim of this study is to determine the prevalence of air microflora on Nigerian
currency
The
objectives are;
1. To
isolate bacteria and fungi present on the Nigerian currency.
2. To
identify the isolated microorganisms associated with Nigerian currency
3. To
determine the percentage occurrence of isolates.
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