ESTIMATION OF GENETIC PARAMETERS FOR GROWTH, EGG QUALITY, AND HAEMATOLOGICAL CHARACTERISTICS OF CROSSBRED JAPANESE QUAIL IN HUMID TROPICAL ENVIRONMENT

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ABSTRACT


This study was conducted to estimate the genetic parameters for growth, egg quality, and haematological characteristics of crossbred Japanese quails in humid tropical environment. A total of three hundred and sixty (360) day old chicks comprising of ninety (90) crossbred for each genotype were used for the study. Each genotype was replicated three (3) times with 30 birds per replicate, the mating ratio was 1:3. Data on fertility and hatchability, body weight and linear body traits, external and internal egg quality traits and haematological parameters were measured for each genotype. The data obtained were subjected to analysis of variance and correlation analyses in a randomized complete blocked design and significant means were separated using Duncan new multiple range test. They were also subjected to regression analysis and estimation of repeatability and heritability as appropriate. Results indicate that fertility and hatchability parameters were statistically (P>0.05) similar among the four strains. PWxCB progenies had the heaviest BWT (169.40 g) in week 9. They also recorded highest values in the following linear body parameters in cm; shank length (SL) 3.55, thigh length (TL) 4.57, body length (BL) 14.14, wing length (WL) 8.96, breast length (BRT) 7.88, and keel length (KL) 4.94 at week 9. A rapid increase in the egg weight (EWT), was found from week 6 of age to week 14 (8.28-12.06) g in PWxCB progenies. Significant and non significant differences existed in external and internal egg quality as well as in haematological characteristics measured. Age at first egg was 42 days in all the genotypes except PWxPW progenies (56) day. At weeks 6 and 8, CBxPW progenies showed positive and significantly strong phenotypic correlations (P<0.01) between BWT and SL (0.518), BWT and TL (0.720). Phenotypic correlations among the external egg quality traits of PWxPW progenies at week 10 showed positive and significant correlations (P<0.05, P<0.01) between EWT and EL (0.462), and EWT and SW (0.415).Genetic correlations between body weight and breast length ranged from 0.135- 0.944 while that of egg weight and shell weight ranged from 0.194 -0.334 in CBxPW strain. The best predictor of body weight were; KL, WL, and BRL with coefficient of regression (R2) of 92.40, this was found in PWxCB at week 6. The four strains evaluated showed high repeatability for body weight across the weeks. The heritability for body weight at week 2 for the progenies were: PWxPW (0.90), PWxCB (0.99), CBxPW (0.98), and CBxCB (0.70). Estimates of heritability for mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC) were high in PWxPW (0.62, 0.67) and PWxCB (0.61, 0.40) respectively. Finally at week 14, heritability for EWT for the progenies were: PWxPW (0.65), PWxCB (0.65), CBxPW (0.99), and CBxCB (0.98). This indicates huge influence of addictive genes. Therefore, selection effectiveness will be high at these weeks. It can then be concluded that PWxCB strain performed best in most of the parameters measured and therefore should be selected for enhanced breeding program for both egg and meat production in the humid tropic of Nigeria.








TABLE OF CONTENTS


Cover page                                                                                                                  PAGE

Title page                                                                                                                    i

Declaration                                                                                                                 ii         

Certification                                                                                                               iii

Dedication                                                                                                                  iv

Acknowledgements                                                                                                    v

Table of Contents                                                                                                       vi

List of Tables                                                                                                              x

List of Plates                                                                                                               xiii

Abstract                                                                                                                      xiv

 

CHAPTER 1: INTRODUCTION                                                                                       1

1.1           Statement of Problem                                                                                                 3

1.2           Objectives of Study                                                                                                    3

 

1.3           Justification of Study                                                                                                  4

CHAPTER 2: LITERATURE REVIEW                                                                            5

 

2.1       Brief history of Japanese Quail                                                                                 5

 

 

2.2       Species of Quail                                                                                                         5

 

 

2.3       Housing of Japanese Quail                                                                                                     6

 

2.4       Factors affecting Fertility and Hatchability performance of Japanese Quail                        7

 

 

2.5       Incubation Procedures of Japanese Quail                                                                             8

 

2.6Turning of Quail Eggs                                                                                                                        8

 

2.7       Hatching Time of Japanese Quail                                                                    9

2.8         Ventilation, Position, Temperature and humidity requirement during

incubation of Japanese Quail                                                                         9

 

2.9         Body weight and Linear Body Parameters of Japanese Quail                                 9         

2.10    Internal Egg Quality Traits                                                                                          11

2.11     External Egg Quality Traits                                                                            12

2.12     Body weight and Egg Quality Parameters of Japanese Quail                                  13

2.13     Genetic and Environmental Influences on Live Weight of Japanese Quail            16

2.14     Nutrient Requirements for Optimum Performance in Quail                         17

2.15     Genetic Parameters of Feed Utilization in Japanese Quail                           18

2.16     Effect of Maternal Body weight of Quails on Progeny Performance             18         

2.17     Heritability of Japanese Quail                                                                                 19                               

2.18     Genetic Parameters for Egg Quality Traits                                        20

2.19     Haematological Traits of Japanese Quail                                                      22

2.20     Repeatability Estimates of Egg Quality Traits of Japanese Quail                        24

2.21     Potentials of Japanese Quail Production in Nigeria                                      25

2.22     Challenges of Japanese Quail Production in Nigeria                                    26

                                                                                                                                                                                     

CHAPTER 3:   MATERIALS AND METHODS                                                                     27

 

3.1        Experimental Location                                                                                   27       

3.2        Breeding Stock                                                                                               27


3.3       Experimental Diets, Egg setting, Breeding Stock and Chick Management   28                               

3.4 Parameters Measured                                                                               32

 

3.4.1    Percentage Fertility of Eggs                                                                          32       

                                                                                   

3.4.2    Percentage hatchability                                                                                    32

3.4.3    Percentage dead in germ                                                                                32

3.4.4    Percentage dead in shell                                                                                 32

3.4.5    Number of chicks emerge                                                                              32

3.4.6    Number of chicks piped                                                                                 32

3.4.7    Mortality                                                                                                         32

3.4.8    Growth parameters                                                                                         33

3.4.8.1 Body weight                                                                                                   33

3.4.8.2 Linear body traits                                                                                                   33

3.4.8.3 Shank length                                                                                                           33

3.4.8.4 Thigh length                                                                                                            33

3.4.8.5 Breast width                                                                                                            33

3.4.8.6 Keel length                                                                                                             33

3.4.8.7 Wing length                                                                                                            33

3.4.8.8 Body length                                                                                                            33

3.4.9 Growth performance traits                                                                                        33

3.4.9.1 Feed intake                                                                                                             33

3.4.9.2 Weight gain                                                                                                    34

3.4.9.3 Feed conversion ratio                                                                                     34

3.4.10 Egg quality parameters measured                                                                   34

3.4.10.1 Egg weight                                                                                                    34

3.4.10.2 Egg length                                                                                                     34

3.4.10.3 Shell thickness                                                                                              34

3.4.10.4 Shell weight                                                                                                  34

3.4.10.5 Egg width                                                                                                      34

 

3.4.10.6 Yolk height                                                                                                   34

 

3.4.10.7 Yolk weight                                                                                                           35

3.4.10.8 Albumen weight                                                                                                   35

3.4.10.9 Yolk index                                                                                                            35

3.4.10.10 Albumen height                                                                                                  35

3.4.10.11 Albumen diameter                                                                                              35

3.4.10.12 Yolk width                                                                                                          35

3.4.10.13 yolk length                                                                                                          35

3.4.10.14 Haugh unit                                                                                                          35

3.4.11 Haematological parameters                                                                                     36

3.5 Experimental Design                                                                                                    36

3.6 Data Analysis                                                                                                                37

3.6.1 Phenotypic correlation and genetic correlation between traits                                 37

3.6.2 Multiple linear regression traits                                                                                                                                 38

3.6.3 Analysis of variance and estimation of heritability                                                                                                                       38

3.6.4 Estimation of repeatability                                                                                                                     39

CHAPTER 4: RESULTS AND DISCUSSION                                                      41

 

                                                                                               

CHAPTER 5: CONCLUSION AND RECOMMENDATIONS                           123     

 

5.1 Conclusion                                                                                                                     123

 

5.2 Recommendations                                                                                                         125

                                                                                   

 

REFERENCES                                                                                                         126

 

  

 

 

 

 

 

LIST OF TABLES

 

 3.1:     Nutrient composition of Japanese quaildiet fed at Starter phase 1-3

            Weeks of age                                                                                                  30       

 

 3.2:     Nutrient composition of Japanese quail diet fed at Layer phase6-14

            Weeks of age                                                                                                  31

 

4.1:      Fertility and hatchability traits of four strains of Japanese quail                        41

4.2:      Growth performance characteristics during the growing and laying

            Phases of four strains of Japanese quail from 1-14 weeks of age                        43

4.3:      Reproductive performance of four strains of Japanese quail                         45

4.4:      Body weight and linear body traits of four strains of Japanese quail

            from 2-9 weeks of age                                                                                                46

4.5:      External egg quality traits of four strains of Japanese quail from

            6-14 weeks of age                                                                                           50       

 

4.6:      Internal egg quality traits of four strains of Japanese quail from

            6-14 weeks of age                                                                                           53

4.7:      Haematological values of four strains of Japanese quail at

            3 and 6 weeks of age                                                                                      57

 4.8      Phenotypic correlations among the body measurements in PWxPW

            strain of Japanese quail from 2-8 weeks of age                                             61

4.9:      Phenotypic correlationsamong the body measurements in PWxCB

            strain of Japanese quail from 2-8 weeks of age                                             63

4.10:    Phenotypic correlationsamong the body measurements in CBxPW

strain of Japanese quail from 2-8 weeks of age                                             66

4.11:    Phenotypic correlationsamong the body measurements in CBxCB

strain of Japanese quail from 2-8 weeks of age                                             68

4.12:    Phenotypic correlations among external egg quality traits in

            PWxPW strain of Japanese quail at 10 and 14 weeks of age                         70

 

4.13:    Phenotypic correlations among external egg quality traits in

            PWxCB strain of Japanese quail at 6, 10, and 14 weeks of age                     71

 

4.14:    Phenotypic correlations among external egg quality traits in

            CBxPW strain of Japanese quail at 6, 10 and 14 weeks of age                         73

 

 

4.15:    Phenotypic correlations among external egg quality traits in

            CBxCB strain of Japanese quail a 6, 10 and 14 weeks of age                     75

 

4.16:    Phenotypic correlations among internal egg quality traits in

            PWxPW strain of Japanese quail from 6 – 14 weeks of age                       77

 

4.17:    Phenotypic correlations among internal egg quality traits in

            PWxCB strain of Japanese quail from 6 -14 weeks of age                         79

 

4.18:    Phenotypic correlations among internal egg quality traits in

            CBxPW strain of Japanese quail from 6 -14 weeks of age                         82

 

4.19:    Phenotypic correlations among internal egg quality traits in

            CBxCB strain of Japanese quail week 6 – 14 weeks of age                       85

 

4.20:    Genetic correlation between body weight and body linear traits in

            PWxPW strain of Japanese quail from 2-8 weeks of age                           87

 

4.21:    Genetic correlation between body weight and body linear traits in

            PW×CB strain of Japanese quail from 2- 8 weeks of age                           89

 

4.22:    Genetic correlation between body weight and body linear traits in

            CB×PW strain of Japanese quail from 2- 8 weeks of age                            91

 

4.23:    Genetic correlation between body weight and body linear traits in

            CB×CB strain of Japanese quail from 2 -8 weeks of age                             93

 

4.24:    Genetic correlations between egg weight and some external egg

            quality traits in PW×PW strain of Japanese quail at 10 and 14

            weeks of age                                                                                                 95

                          

4.25:    Genetic correlations between egg weight and some external egg

            quality traits in PW×CB strain of Japanese quail at 6, 10 and 14

            weeks of age                                                                                                 97

 

4.26:    Genetic correlations between egg weight and some external egg

            quality traits in CBxPWstrain of Japanese quail at6, 10 and 14

            weeks of age                                                                                                 99

 

4.27:    Genetic correlations between egg weight and some external egg

            quality traits in CBxCB strain of Japanese quail at week 6, 10 and 14

            weeks of age                                                                                                 101

 

 4.28:   Stepwise multiple linear regression of body weight on linear body traits of four strains of Japanese quail from 2- 8 weeks of age             103

 

 

 4.29:   Stepwise multiple linear regression of egg weight on external egg

quality traits of four strains of Japanese quail from 6- 14 weeks

of age  106

 

4.30:    Repeatability estimates of body weight and linear body parameters of

            four strains of Japanese quail from 2-8 weeks of age                             108

              

4.31:    Repeatability estimates for external egg quality parameters of

            four strains of Japanese quail from 6-14 weeks of age                          110

 

4.32:    Repeatability estimates for internal egg quality parameters of

            four strains of Japanese quail from 6 -14 weeks of age.                        112

 

4.33:    Heritability estimates for body weight and linear body traits of

            four strains of Japanese quail from 2- 8 weeks of age.                          114

 

4.34:Heritability estimates for haematological parameters of four

            strains of Japanese quail at 3 and 6 weeks of age.                                 116

 

4.35:Heritability estimates for internal egg quality parameters of

            four strains of Japanese quail from 6-14 weeks of age.                         118

 

4.36:    Heritability estimates for external egg quality parameters of four

            strains of Japanese quails from 6- 14 weeks of age.                                     120

                                                                                   

 

 

 

 

 

 

LIST OF PLATES


 1:        Brooding of PWxPW Progenies                                                                              137              

                                              

 2:        CBxPW Progenies                                                                                               137


 3:        CBxCB Progenies                                                                                                138      

                                                                   

 4:        PWxCB Progenies                                                                                               138


 5:        Determination of initial body weight of Japanese quail using

            electronic scale                  139


 6:        Determination of egg length using venier caliper                                               140


 7:        Local cabinet incubator                                                                                       140

 

 

 

 


 

 

 

CHAPTER 1

INTRODUCTION

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In the wild, Japanese quail is a small bird originating from the far East where the birds were first raised in cages in Japan and China around the 15th century due to its singing ability. Japanese quail was brought to Nigeria in 1992 (NVRI, 1994). The newly hatched weighs 6-8 grams but grow rapidly and fully feathered at 4 weeks of age. Mizutani (2003) reported that adult male quail weighs about 100-130 grams. Males also have cloacal glands (a bulbous structure located at the upper edge of the vent which secrets a white foamy material). This unique material is used to ascertain the reproductive fitness of the males. The young male starts to crow at 5-6 weeks old. The adult females are slightly heavier when compared to the males weighing 120-160 grams (Ortlieb, 2013).

Quail rearing for egg and meat production has become an economically important activity in Nigeria and it is increasingly being developed due to increasing interest in the products for food and medicine. As a suitable alternative to chicken, its improvement will help to provide a suitable animal protein to Nigerians particularly among rural populace. Improvement of poultry birds by genetic principle is one of the vital ways in developing the poultry industry. In meat producing animals and birds, growth is the most important trait for evaluating different livestock species. Growth parameters such as bodyweight and bodyweight gain are affected by genetic and non-genetic factors and the phenomenon of growth is usually determined by observing differences in bodyweight recorded at different ages or bodyweight gain obtained during different growth periods (Chambers, 1993).

Determination of conformation variables in chickens such as shank length, thigh length, breast girth and body length is important if any breeding programme would be meaningful and successful. This is applicable to Japanese quail. Some of these conformation traits are good indicators of body weight and linear body measurements,and are very suitable because of their economic importance. Since these traits vary from time to time, knowledge of repeatability estimates will enable the breeder in designing an appropriate breeding plan for their improvement. Falconer (1989) stated that repeatability estimates indicate gain in accuracy expected from multiple measurements. Heritability indicates the amount of improvement that can be achieved by selection. Ude (2010) reported that effect of these breeding programmes is the increase in the proportion of additive genetic variance and improvement of selection response. Linear regression of traits enable us predict efficiently the body weight of the birds especially in rural areas where weighing balance or scale may not be readily available.

The Haematological analysis is undertaken to evaluate the health status of bird along with diagnosis and clinical monitoring of any disease (Kenneth, 2010). Haematological analysis is one method that contributes to detection of some changes in health status which may not be apparent during physical examination, but which affect the fitness of the birds.  Both haematological and biochemical blood values serve as indicators of the physiological state of birds (Driver, 1981). Egg quality is the characteristics of an egg that affect its acceptability by the consumers. Egg quality is the more important price contributing factor in table and hatching egg production. Therefore, the economic success of a laying flock solely depends on the total number of quality eggs produced (Monira et al., 2003).

Information on crosses of Japanese quails relating to heritability, regression, repeatability, phenotypic and genetic correlations in Nigeria is comparatively scanty in literature, hence need for the study.


1.1 Statement of Problem    

The quails in humid tropical environment of Nigeria is generally poor performing and small body size, hence have remained unproductive over the years. This is as a result of their poor genetic profile and lack of improved production environment. This problem has led to inadequate animal production and concomitant low animal protein consumption in Nigeria. The approach towards solving this problem is to improve the genetic-make-up of the quails. A plausible approach to genetic improvement is selection of individuals based on presence or absence of genetic and physiological characters of individuals as a result, hybrid animals will be produced.


1.2 Objectives of Study        

1.     To determine the following:

a.     Growth and some reproductive performance of crossbred Japanese quails.

b.     Fertility and hatchability of Japanese quails.

c.     Egg quality of crossbred Japanese quails.

d.     Haematological characteristics of crossbred Japanese quails.

2.     To estimate the phenotypic and genetic parameters of growth, egg quality, and haematological characteristics of crossbred Japanese quails.

 

1.3 Justification of Study

Evaluation of the performance of animals constitutes an essential part of successful breeding plans for sustainable genetic improvement. Tropical quails have low growth efficiency and small body size. This has brought about increased clamor for improving these quails by adopting proper breeding programme. This will ensure the selection of improved strains which have high reproductive and high growth potential. For crossing to be effective, the selected parents must be of proven performance under prevailing environmental conditions. This will go a long way  towards increasing and improving quail production, thereby helping to improve the deplorable animal production and consumption situation in tropical developing countries specifically Nigeria.

 



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