analysis of water was carried out on well water, stream and pipe
born water obviated within Emene locality of Enugu state the sample were
subjected to some analytical tests final like presumptive tests, comfirmative
test final like presumptive tests, comfirmative test, completed test, indole
test and plate count for the organism that develop on the culture, the result show that well water
and stream were found to contain pathogenic organism like salmonella, Shigella
and staphylococcus aureus as well as numerous viable counts, the cloform
or the indicator organisms Escherichia
coli the family of enterbactercteriaceae were isolated too form well water the
pipe borne water a sample contained staphylococcus aureus and have lowtotal viable counts.
TABLE OF CONTENTS
Table of contacts
List of table
List of figures
CHAPTER ONE: INTRODUCTION
1.1 Aims and objective
1.2 Statement of problem
1.4 Significance of study
CHAPTER TWO LITERATURE REVIEW
3.0 CHAPTER THREE: MATERIALS AND METHOD
3.1 Sample collection
3.3 Sterilization of materials
3.5 Presumptive test
3.6 Confirmed test
3.7 Completed test
3.8 Test for indole
3.9 Methyl red test
3.10 Oxidase test
3.1.1 catalse test
3.12 plate counts techniques
3.13 preparation of single and double strength media
3.14 dilution technique in most
problem number `
3.15 Inoculation of media with
GRAM STAIN TECHNIQUE
4.0 CHAPTER FOUR: RESULTS
4.2 Most problem number (mpn) and pH result.
Chapter five. Discussion an
LIST OF TABLE S
comparative guality of ground water and surface water
colony count result 25
MPN and PH result 25
the MPN of cliform in the
Table V the
physiological biochemical of the isolated organisms 28
I culture plate picture 23
II isolated micro organisms and shape 24
Following the theory of creation, it
is clear that water is as old as man. From time immemorial, man has restorted
to the use of this unique commodity for domestic and other purposes.
Most of our water supplies are from surface water which
include: rivers, streams, lakes, oceans and seas and there water bodies are
likely to be polluted with domestic and Industrial as well as agriculture
waste, As populations increase, the problem become more serious and as such,
water can endanger the health and life of human beings because when polluted by
fecal materials it becomes potential carrier of pathogenic organism (Carpenter
Water, is of course, absolutely essential to life, not only
human life but all life, animal and vegetable. Most of the biochemical reaction
that occur in metabolism and growth of living cells involved water, and all
take places in water. (Camp etal,1974).
Man uses water not only for drinking purposes but also for
bathing, washing, laundering, heating, air conditioning, agriculture, stock
raising and gardens, Industrial processes and cooling water power and steam
power, fire protection, fishing, swimming and wild life propagation and
Natural water contain not only then natural flora but also
micro – organisms from soil and possible from animals or sewage. Surface waters
in streams or pools and stored waters in lakes and large ponds vary
considerably in microbial content. (frazier 1978) water is broadly divided into
three types viz., surface water which include: streams, rivers, lakes sear, and oceans Kelman et al (1957)
ground water, well borehle, many people have defined the ground water in
different ways: ground water is subter water that occurs where all pores in the
soil or rock counting materials are saturated (pelezer et al 1992).
atmospheric water, which includes rain, falls. All water bodies consist of a
variety of bacterial and other microorganisms like the Algaes fungi, which
inhabit these natural water bodies. Some of these micro –organisms are
indigenous to thus natural water while others are transient, entering the water
from external environment (Pelezar and Reg, 1997).
generality of bacteria are mostly commonly found ordinarily in fresh water some
of which include: pseudomonas,
Archacbacter, and vibrio these are gram negative, the gram-positive bacterial
which are found in water include: micrococcus Archacbacter and actinomycentes.
Gebharal (1975) Tap water, as one of the
water sources is mostly used domestically, it is observed that tap change
sometime the water tap will be clear this calls for load, in order to be sure
of its portability. Bonde (1977)
increase in drinking water from different sources especially in Enugu state has
made necessary to investigate the microbial content of water. Water is a
potential carried of pathogenic organisms that can endage human life. Most of
drinking water sources are often contaminated with different pollutants like faces,
animal and plant wastes, making such water
unfit for drinking if not treated. The pollution of water with
pathogenic organisms and other pollutants can only be detected by carrying out
microbiological assessment of such water. Most human disease such as typhoid
paratyphoid cholera, arnboebiasis, Trichinosis, gastroenteritis, sanonall
shigellosis, diphtheria, giadia, dracunculus etc are know to be water borne
disease. Ewington et al (1971).
born disease are those disease which have water as their vehicle of
transmission these disease are capable of destroying a whole community if not
checked. Therefore, the quickest ways to prevent out break of these disease and
to determine the portability of such water sources is to determine the
microbial load or content if the microbial content is nor within acceptable
limit, such water sources should be
condemned immediately. Fair et al (1970)
To find out if well, stream, Tap water coutain
To help the public to know the danger of drinking
these water without adequate treatment.
To compare the microbial load of the water source and
advice on safer source of water .
source of water are contaminated through one way or the other
The short falls in the distribution
of treated pipe borne water leads people
to resort to alternative source of water which may be unfit for human
Tap water can be polluted through leakage’s/ improper
Well water is contaminated as a result of running off
into it, especially shallow well.
Streams can be contaminated through waste from
industries, leaves, dust, and rain run often.
HO- pathogenic bacteria are present in
H1- pathogenic bacteria are not present in
Hiii- The level of pathogenic organism in
water can cause water borne infection
Hilo The level of pathogenic organism in
water can not cause watt born infection.
SIGNIFICANTS OF STUDY
The study will advice on water meet the standard
quality required for any particular purpose
Also advice the eswc on quality of their product. For
future nature modification of treatment methods
sample were worked on because of lack of fund, the survey is however limited to
the bacteria flora of waters, and not all n organisms even on bacterial flora
specification the number of sample culture were limited by lack of funds and
culture media is expensive.
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