PROXIMATE AND ANTI NUTRIENTS EVALUATION OF MIX-GRUEL BLENDS OF WHEAT (TRITICUM GENUS), GROUNDNUT( ARACHIS HYPOGEA), SOYBEAN (GLYCINE MAX) AND DRIED FISH (HOPHIUS PISCATORIES SPP.) FOR BREAKFAST

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Product Code: 00009417

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ABSTRACT

The study evaluated the proximate and anti-nutrients composition of the mix-gruel blend of wheat, groundnut, soyabean and dried fish for breakfast. This study was done using six objectives and six research questions. Production of dried mix-gruel blend was formulated based on different percentages. The study adopted experimental research design to ascertain the proximate content, anti-nutritional content and sensory attributes. The population of the study was three hundred and eighty-seven staff and students from the Home Science/Hospitality Management. Twenty-three persons served as the taste panellists and were selected by multi-stage sampling techniques. Data obtained was subjected to statistical analysis of variance (ANOVA). The sensory result from the twenty three panelists revealed that there was no significant different in the appearance, texture, taste and mouthfeel all the samples assessed. Sample “B” (50% wheat, 20% groundnut, 20% soyabean,10% fish) has the highest score on general acceptability. The result of proximate composition of the blends of different samples showed that, there was a significant difference (P > 0.05) in ash, moisture, carbohydrates, crude fat, crude fibre and protein content. The result of anti-nutritional factor was found to be low in the different sample of the blend indicating possible bioavailability of the nutrient. The micro-nutrient results showed that the there was a significant difference (P<0.05) in the samples of blend. Based, on the findings of this study, it was recommended, that families can introduce this mix-gruel in their menu. The mix-gruel is richer in protein, carbohydrate, fat, vitamins, mineral and can as well help fight this double burden of protein-energy malnutrition and micro-nutrient deficiencies.






TABLE OF CONTENTS


Title Page                                                                                                                                i

Declaration                                                                                                                             ii

Certification                                                                                                                           iii

Dedication                                                                                                                              iv

Acknowledgements                                                                                                                v

List of Tables                                                                                                                          vi

Abstract                                                                                                                                  vii


CHAPTER 1: INTRODUCTION                                                               

1.1       Background to the study                                                                                             1

1.2       Statement of the Problem                                                                                           4

1.3       Objective of the Study                                                                                                5

1.4       Research Questions                                                                                                    6

1.5       Hypotheses                                                                                                                 6

1.6       Significance of the Study                                                                                           7

1.7       Scope of the Study                                                                                                      8


CHAPTER 2: LITERATURE REVIEW                                                  

2.0       Conceptual Framework                                                                                              10

2.1       Nutrient Values of Cereals-legume Foods                                                                 10

2.1.2    Nutrient component of dry-fish                                                                                  14

2.1.3    Wheat and its utilization                                                                                            15

2.1.4    Groundnut and its utilization                                                                                      16

2.1.5    Soybeans and its utilization                                                                                        18

2.1.6    Dietary pattern                                                                                                            19

2.1.7     Factors influencing dietary patterns                                                                          20

2.1.8    Assessment of dietary patterns                                                                                  20

2.1.9    Importance of studying dietary patterns                                                                    21

2.1.10  Proximate analysis                                                                                                      21

2.1.10.1 Basic component of proximate analysis                                                                   22

2.1.11  Anti-nutritional factors in plant foods                                                                                    22

2.1.12 Processes of reducing anti-nutrient factors in plant foods                                         24

2.1.13  Micronutrient malnutrition                                                                                         26

2.1.14  Protein-energy malnutrition                                                                                       30

2.1.15  Food blending                                                                                                             31

2.1.16  Sensory evaluation                                                                                                     32

2.1.17  Achieving food system sustainability and food security                                           33

2.2       Theoretical Framework                                                                                              34

2.2.1    Food production theory                                                                                              34

2.2.2    Conceptual blending theory                                                                                       35

2.3       Review of Related Empirical Studies                                                                         35

2.4       Summary of Literature Reviewed                                                                              41

 

CHAPTER 3: MATERIALS AND METHOD                             

3.1       Design of the Study                                                                                                    44

3.2       Area of the Study                                                                                                        44

3.3       Population for the Study                                                                                             45

3.4       Sample and Sampling Techniques                                                                             45

3.5       Procurement of Raw Materials                                                                                   45

3.5.1    Processing of Raw Material                                                                                       46

3.5.2    Sample Formulation                                                                                                   46

3.5.3    Sample Preparation                                                                                                    47

3.6       Instrument of Data Collection                                                                                    47

3.7       Validation of the Instrument                                                                                      47

3.8       Permission                                                                                                                  47

3.9       Data Collection Techniques                                                                                       48

3.10     Proximate Analysis                                                                                                     48

3.10.1 Determination of Moisture                                                                                         48

3.10.2  Determination of Ash                                                                                                 49

3.10.3  Determination of Crude Fat                                                                                        49

3.10.4  Determination of Crude Fibre                                                                                    50

3.10.5  Determination of Crude Protein                                                                                 51

3.10.6  Determination of Carbohydrate                                                                                  53

3.11     Determination of Anti-nutrient                                                                                   54

3.11.1 Determination of Oxalate Content                                                                              54

3.11.2  Determination of Tannin                                                                                            54

3.11.3  Determination of Alkaloid                                                                                          55

3.11.4  Determination of Phytic Acid                                                                                     56

3.11.5  Determination of Flatulence Factors                                                                          57

3.11.6  Determination of Phenol                                                                                            57

3.12     Micro-nutrients                                                                                                           58

3.12.1  Calcium Determination                                                                                              58

3.12.2  Determination of Iron                                                                                                 59

3.12.3  Determination of Zinc                                                                                                59

3.12.4  Determination of Iodine                                                                                             60

3.12.5  Determination of Magnesium                                                                                     60

3.12.6  Determination of Vitamin A                                                                                       61

3.12.7  Determination of Vitamin C                                                                                       62

3.12.8  Determination of Vitamin K                                                                                       63

3.12.9  Determination of Vitamin D                                                                                       63

3.12.10 Determination of vitamin B1(Thiamine)                                                                   63

3.12.11 Determination of Riboflavin (Vit B2)                                                                        65

3.12.12  Determination of Niacin content (Vit B3)                                                                66

3.12.13 Determination of Vit B6 and B12                                                                               67

3.12.14 Determination of vitamin E                                                                                       67

3.13     Statistical Analysis                                                                                                     68


CHAPTER 4: RESULTS AND DISCUSSION

4.1       Findings of the Study                                                                                                  69

4.2       Discussion of Findings                                                                                               78

4.2.1    Proximate compositions of the blends                                                                        78

4.2.2      Anti-nutrient contents of the samples                                                                         80

4.2.3      Micro-nutrient composition of the blends                                                                  82

4.2.3.1  Vitamin composition of the blends                                                                            82

4.2.3.2 Mineral composition of the blends                                                                             83

4.2.4      The sensory attributes and consumer acceptability of the

formulated mix-gruel blend                                                                                        85

 

CHAPTER 5: SUMMARY, CONCLUSION AND RECOMMENDATIONS

5.1       Summary                                                                                                                    86

5.1.1    Major finding                                                                                                              88

5.2       Conclusion                                                                                                                  88

5.3       Recommendations                                                                                                      88

5.4       Contribution to Knowledge                                                                                        89

5.5       Suggestion for Further Study                                                                                     89

            References                                                                                                                  90

            Appendices                                                                                                                 98

 

 

 

 




 

LIST OF TABLES

1:         Proximate composition of the blends                                                                         72

 

2:         Anti-nutritional composition of mix-gruel blends of different

percentages                                                                                                                 73

 

3:         Micronutrient composition of the blends vitamins composition

of the blends                                                                                                               74

 

4:         Mineral composition of the blends                                                                             75

 

5:         Sensory attributes and consumer acceptability of the formulated

mix-gruel blend                                                                                                          76

 

 

 


 

CHAPTER 1

INTRODUCTION


1.1       BACKGROUND TO THE STUDY

Breakfast is well acknowledged to be the most important meal of the day, because it provides immediate fuel and nutrient for utilization by the brain after an overnight fast. It helps the body to kick off the day both mentally and physically. Breakfast meal is vital for the body functionality and promotes activity level for the day. The body system needs food to function well. It is often observed that after some hours without food, the brain, tissues and muscles will be short of energy to function well. Taking breakfast boasts one's metabolism and increases one's energy throughout the day. Breakfast meals are likely to provide favourable nutrient intake including higher intake of dietary fiber, proteins, total carbohydrates, fat and cholesterol. It makes large contribution to daily micro-nutrient intake, (Balvin, Trevino, Echo and Grarcia, 2013). Consumption of inadequate breakfast is a factor in contributing to poor health status. The need to exploit unexploited and underutilized food resources for breakfast meal is very essential. (Enujiugha and Agbede, 2000).   

Mix-gruel is a combination of different food commodities to produce a meal. Gruels are paste and semi-solid meal formulated from varying food commodities especially grains. Most commonly eaten breakfast gruel meals in Nigeria are pap, commonly referred to as akamu by Igbos, ogi by Yorubas and koko by Hausas. It could be made from maize, millet, sorghum, which may not satisfy the energy and other nutrient needs of the body system. The gruel if too watery/liquid in consistency may have low energy. This most popular traditional breakfast gruel "Akamu" for instance is pre-dominantly produced from fermented maize. The major disadvantage of this cereal gruel is that the starchy nature of these foods makes them bind so much water, thus yielding a bulky gruel with decreased nutrient content. The promotion and inclusion of more plant protein especially soyabean and groundnut in the staple diets will be nutritionally beneficial. (Barber, Ejiofor, Eke, 2010). Flour produced from either cereals and legumes will have a nutritional value inferior to those produced from a combination of both.

Producing this mix-gruel with cereal-legume blends rather than using independent food commodities, will be more nutrient dense. Supplementation of cereals with legumes has been suggested as one way of improving the protein quality of cereal-based gruel. Barber (2010) stated that, to improve the nutritional value of cereal-based diets, legumes should be used to fortify them. The nutrient content of legumes makes them natural complements to cereal-based diets. Efforts to improve the nutritional value of these staples have been based on fortification with legumes to boast the deficient amino-acid. Combination of cereals and legumes can be desirable from a nutritional stand point, because of the complementation of essential amino acids and increase in protein content. The mixture of wheat, soyabean, groundnut will be a good nourishing meal, providing protein, energy, dietary fibre and micro-nutrient. The essential amino acids and omega-3-fatty limiting in cereals-legume are present in fish. A full-breakfast meal containing these food nutrients will help increase the nutrient content.

Breakfast meals are thought to provide good nutrition, (Nathan, 2008). The basic requirements of a man, is his right to adequate and sufficient food. Nutrition is a basic human right. Nutrition is the study of food at work in the body, the source for energy and a medium for which the nutrients can function. It is the building block of life. Nutrition ensures vitality and functionality of the body system through foods. It is the means of getting the right amount from healthy foods in the right combination. "No wonder" Thomas Edison" quotes “the doctor of the future will give no medicine but will be interested in his patient in care of human frame, in diet and in the cause and prevention of diseases”. Good nutrition significantly reduces the risks of sickness and mortality in the context of host of diseases, as well as maternal health and foetal growth. Inadequate nutrition early in life and amongst the elderly can result in irremediable damages to the developing brain and body of children as well as complication in the elderly. Producing this home-made inexpensive, nutrient dense mix-gruel from local food source for breakfast meal that will offer good nutrition becomes apparent. The production of mix-gruel for full-breakfast meal will add increase to food that people consume.                     

These home-made mixed-gruel produced with soybean (Glycine max), which is a rich source of protein and oil (Nkama, 1995) soyabean is limiting in sulpur containing amino-acids for most animals species including humans but contain sufficient lysine to help overcome the lysine deficiency of other cereals. Groundnut (Arachis hypogea) is a versatile pulse with rich, cheap and good source of vegetable protein available to mankind. It contains high protein, high polyunsaturated fat with absence of cholesterol and lactose, an amino acid vital for body growth and reproduction. Wheat is a cereal based food commodity with high protein gluten which will serve as binding agent in this new food production. Fish is a source of protein and micro-nutrients, very high in calcium, iron, zinc and vitamins A. Fish is the primary sources of long-chain omega-3-poly unsaturated fatty acid with numerous health benefits including improved infant cognitive and visual development, reduced risk of cardiovascular diseases, fish will help enrich the nutrient lacking in plant foods, (Walter, 2012).

This study is geared towards evaluating the proximate composition of different samples of the blends. Proximate analysis is the estimation and determination of how much of the major food components namely moisture, carbohydrates, protein, ash, crude fibres that are contained in this mix-gruel blend. The plant food utilized in this mix-gruel blend contains anti -nutritional factors which are responsible for the deleterious effect that are related to the absorption of nutrient and micro nutrient which may interfere with the function of certain organs. Anti-nutrients such as oxalate, tannins, phylate, protease inhibitor many cause undesirable effects in human, if their consumption exceeds normal levels recommended for human consumption. The harmful effect may result due to breakdown of the food product and there is need to ascertain the anti-nutritional factors in this mix-gruel blend to ensure conformance to wholesomeness. The mix-gruel blend will be subjected to sensory evaluation to ascertain the attributes such as color, texture, flavour, odour and overall acceptability on 9-point hedonic scales.            

In all, the study examined the proximate and anti-nutrient evaluation of mixed gruel blends of wheat, groundnut, soyabean, dried fish for full-breakfast meal.


1.2       STATEMENT OF THE PROBLEM

Most breakfast meals are cereals based foods that are highly carbohydrate such as gruel from millet and maize which may not satisfy the nutrient needs of the body system. The need for improvement of the nutritional quality of cereals by complementing their limiting amino acid with legumes which will help improve the protein and nutrient density of subsequent foods. Since there is scanty information available on their nutritional quality, anti-nutritional properties and also poor utilization of these food commodities in different food formulation, combination of cereals with legumes will improve the protein and nutrient density of food.

Most households are troubled with what to serve as breakfast, as time constraints preparation of nutritionally adequate breakfast. Families often resolve to quick or convenience source such as cereal or bread which often time are not adequate as they do not contain all the required food nutrients, required for complete meal.

Adding to enrich the blends, fish with micronutrient and protein helps to supplement the nutrient lacking in legumes and cereal. They are significant sources of energy, protein dietary fiber, vitamins, minerals, antioxidants and phytochemicals. Production of the mixed-gruel blends instead of single gruel will serve as a preventive meal for protein-energy-malnutrition and other disease. The production of mix-gruel with wheat, groundnut, soybean, dried fish blend will provide addition to existing breakfast menu available for consumption.

The study is conceived to provide a rich variety to prevent the monotonous gruels commonly served as breakfast meals and also help to solve the double burden of hunger and malnutrition in line with the second SDG (End hunger, achieve food security and improved nutrition and promote sustainable agriculture.


1.3       OBJECTIVE OF THE STUDY

The general objective of the study was to evaluate the proximate and anti-nutrient composition of mixed gruel produced from wheat, groundnut, soybean and dried fish for full breakfast meal.

The specific objectives of the study include to;

1.     produce flour using wheat (Triticum genus), groundnut (Arachis hypogeal), soybean (Glycine max) and dried fish;

2.     produce a mix-gruel mixture from different flour blends;

3.     determine the proximate composition of the blend;

4.     determine anti-nutrient composition of the mix-gruel blend;

5.     determine micro-nutrient of the mix-gruel blend;

6.     evaluate the sensory attributes and acceptability of the formulated mix-gruel blend.


1.4       RESEARCH QUESTIONS

Based on the objectives, above the following research questions were formulated to guide the study.

1.     How can a flour sample blend be produce from the wheat, groundnut, soyabean and dried fish.

2.     How can a mix-gruel mixture of different blend be produced?

3.     What is the proximate composition of the blends?

4.     How can the anti-nutrient of the blend be determined?

5.     How can the micro-nutrient of the blend be determined?

6.     To what extent would the blend be acceptable?

7.     How will the blend be packaged and labelled?

1.5       HYPOTHESES

With the research questions formulated above the underlisted H0 were raised to support the study.

H01: There is no significant difference between produced mix-gruel powder of the food commodities and the powder of commercial quaker oat meal (control)

H02: There is no significant difference in the produce mix-gruel mixtures from different blend and commercial quaker oat meal (control).

H03: There is no significant differences between the acceptability of the produced mix-gruel mixture and commercial quaker oat meal (control).

1.6       SIGNIFICANCE OF THE STUDY

The findings of this study will be of great importance to Students, Home Economist extension workers, Nutritionist and food scientist, Agriculturalist extension worker and Food producers.

The outcome of this project will be useful to the students because it will serve as reference source to other research into development of new mix-gruel blend with locally made available food commodities. This study can be accessed through journal publications and internet uploads.

This study will be of benefit to home economist extension workers, using the findings of the study to teach mothers and families on how to prepare mix-gruel blends. For breakfast meal that are nutrient-dense as well improve their feeding capacity. This can be achieved through visiting them to educate the women and families on community town hall meetings, church meetings and women August meeting.

The findings will be significant to nutritionist and food scientists as it will provide them new information on the production of nutrient-dense breakfast meal using available local food commodities which can assist to compile food composition-table for use in breakfast meal. The result will be made known to them through publication in the internet and journals.

This study will be of benefit to Agriculturalist extension workers, will use the result of the findings to encourage farmers to produce more of these food commodities to ensure availability and surplus for sale to generate income as well promote food security. This will be made available to farmers through community town hall meeting.

 Finally, the study will be also of benefit to food producers. The result of the finding will encourage food producer to produce mix-gruel based on cereal- legume and fish blends that are nutrient-dense.


1.7       SCOPE OF THE STUDY

This research on proximate and anti-nutrient evaluation of homemade mix-gruel blend produced from a combination of wheat, groundnut, soybean and dried fish for full-breakfast meal focus on production of  a dried sample flour using wheat, groundnut, soyabean and dried fish. The study produced a mix-gruel mixture form the different flour blends, evaluated the proximate composition of the blend; anti-nutrient composition of the blend; sensory attributes and consumer acceptability of the formulated mix-gruel blend; package and label the produced mix-gruel. This study was be carried out in Home Science and Food Science laboratories of Michael Okpara of Agriculture, Umudike, Abia State. 

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