EFFECT OF ABATTOIR WASTE ON THE ABUNDANCE AND DISTRIBUTION OF PLANKTON IN IMO RIVER, OYIGBO, NIGERIA

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ABSTRACT


The study identified the effect of abattoir waste on the abundance and distribution of plankton of the increasingly abattoir activities in Imo River, Oyigbo, a Delta area of Nigeria, as indicators of the health of the aquatic ecosystem. Plankton samples were collected once monthly for 10 months (January - October 2017).  Result showed monthly variations of Physico-Chemical parameters. Means of monthly values of pH ranges between 6.00-7.12, temperature ranged between 18 – 240C, and electrical conductivity was between 273.33µs/cm – 540.00µs/cm . Dissolve oxygen (DO), Dissolved solid (DS) and Biological oxygen demand (BOD) Means  of monthly values ranges between 3.53±0.03 mg/l – 6.00±0.31mg/l, 103.67±5.78mg/l - 131.00±12.01mg/l, and 5.10±0.10mg/l – 6.83±0.20mg/l respectively. Four Phytoplankton phyla were recorded, they were dominated by Chlorophyceae made up of Scenedesmus sp, Spirogyra sp, Ulothrix sp, Volvox sp, with Spirogyra sp in abundance in station 1 and a decrease of the specie through station three, followed by Cyanophyceae made up of Microcystis sp, Oscillatoria sp, Gomphosphaeria sp, Anabaena sp and dominated by Microcystis sp in station one (Upstream of the river) as it reduces significantly across other stations followed by Euglenophyceae with only Euglena sp recorded across all stations having its highest abundance in station one and Baccillariophyceae which comprises of Cyclotella sp, Cymbella sp, Diatomella sp with its dominance at station (3) three and Cyclotella sp having highest abundance. The Zooplankton of the River were made up of Protozoa: (Paramecium sp, Pelomyxa sp, Acanthometron sp) having its abundance in station three, Rotifers (Keratella sp, Branchionus sp, Monostyla sp, Euclanis sp) and Cladocera(Daphnia sp, Microcyclop sp, Bosmina sp).   Correlation matrix showed that there were significant correlation between Phytoplankton, Zooplankton and Physico-Chemical parameters. Plankton abundance peaked in station 1(842 organisms/ml) and was least in station 3. The dominating presence of Chlorophyceae shows gradual deterioration of the water quality. This could be as a result of abattoir activities, such as the wastes washed into  the river. The low abundance and distribution recorded could be attributed to growth-limiting perturbations created by ongoing abattoir activities in water columns.





TABLE OF CONTENTS

Title Page                                                                                                                    i

Declaration                                                                                                                  ii

Certification                                                                                                                iii

Dedication                                                                                                                  iv

Acknowledgements                                                                                                    v

Table of Contents                                                                                                       vi

List of Tables                                                                                                              viii

List of Plates                                                                                                               ix

List of Figures                                                                                                             x

Abstract                                                                                                                      xi


CHAPTER ONE: INTRODUCTION

1.1        Background of the study                                                                                1

1.2       Statement of the Research Problem                                                                4

1.3        Justification                                                                                                     4

1.4.      Aim and Objectives of Study                                                                         5

1.5       Significance of the Study                                                                               5

1.6.      Scope and Limitations                                                                                                5


CHAPTER TWO: LITERATUREREVIEW

2.1        Studies on Abattoir Waste                                                                              6

2.1.1        Abattoir waste on surface water                                                                     6

2.2       Physico-Chemical parameters                                                                         10

2.2.1    Temperature                                                                                                    10

2.2.2    Turbidity                                                                                                         11

2.2.3    Water Ph                                                                                                         12

2.2.4    Water hardness                                                                                               13

2.2.5    Dissolved oxygen (DO)                                                                                  13

2.2.6    Biochemical oxygen demand (BOD)                                                             14

2.2.7    Electrical conductivity of water                                                                     14

2.2.8    Total dissolved solids (TDS)                                                                          15

2.3       Biological Parameters                                                                                      15

2.3.1    Studies on phytoplankton                                                                               15

2.3.2    Studies on zooplankton                                                                                  17

CHAPTER THREE: MATERIALS AND METHODS

3.1       Study Area                                                                                                      19

3.1.1      Sampling stations                                                                                            21

3.1.2    Sampling Procedures                                                                                      21

3.2       Physico-Chemical Parameters                                                             22 

3.2.1           Determination of pH                                                                                           22

3.2.2        Determination of water temperature                                                               22

3.2.3        Determination of dissolved oxygen (DO) and biochemical oxygen demand (BOD)                            22

3.2.4        Determination of electrical conductivity, dissolved

solids (DS) and suspended solid (SS)                                    23

3.3       Biological Parameters                         23

3.3.1    Determination of phytoplankton                                                                    23

3.3.2    Determination of zooplanktons                                                                      24

3.4       Community Structure Analysis                                                                       24

3.4.1    Margalef index of species richness                                                                 24

3.4.2    Shannon index                                                                                                            25

3.4.3.  Community dominance index (CDI)                                                              25

3.5       Statistical Analysis                                                                                          25

CHAPTER FOUR: RESULTS AND DISCUSSION

4.1       Physicochemical Parameters                                                                           26

4.1.1    Relationship between phytoplankton, zooplankton and some physico chemical parameters                                    29

4.1.2    Biological indices of plankton (shannon-weiner,

dominance and margalef index)                                         31

4.2       Phytoplankton                                                     32

4.2.1    Cyanophyceae                                                                                                 32

4.2.2    Chlorophyceae                                                                                                32

4.2.3    Euglenophyceae                                                                                              37

4.2.4    Bacillariophyceae                                                                                            37

4.3       Zooplankton                                                                                                    38

4.3.1    Euglenophyceae                                                                                              38

4.3.2    Bacillariophyceae                                                                                            39

4.3.3    Protozoa                                                                                                          39

4.3.4    Rotifers                                                                                                           39

4.3.5    Cladocera                                                                                                        39

4.4      Discussion                                                                                                        45

4.4.1    Plankton abundance and distribution                                                             45

4.5       Physico-Chemical Parameters                                                             46

4.6       Plankton Composition                                                                                    47

4.7       Health Implication of the Contaminated Water                                             49

4.8       Surface Water Contamination and Domestic Water Uses in Study Area      50

CHAPTER FIVE: CONCLUSION AND RECOMMENDATION

5.1       Conclusion                                                                                                      51

5.2       Recommendations                                                                                          51

References                                                                                                      52

Appendices                                                                                                     62

 

LIST OF TABLES

 

4.1       Mean values of Physico-chemical parameters of Imo River during

the months of January to October, 2017                                                        28

4.2       Correlation matrix showing relationship between zooplankton, phytoplankton and some physico-chemical parameters                                  30

4.3       Phytoplankton Species Indices                                                                       31

4.4       Zooplankton Species Indices                           31

4.5       Mean number of phytoplankton population in Imo River, Oyigbo recorded at different months                     38

4.6       Mean number of Zooplankton population in Imo River recorded at different months                                             43

 

LIST OF FIGURES

 

3.1       Map showing sampling station                                        20

 

4.1       Mean monthly variation of Cyanophyceae in Imo River,

Oyigbo in relation to Months and Stations                                 33

 

4.2       Mean monthly variation of Chlorophyceae in Imo River,

Oyigbo in relation to Months and Stations                                                     34

 

4.3       Mean monthly variation of Euglenophyceae in Imo River, 

Oyigbo in relation to Months and Stations                                                     35

 

4.4       Mean monthly variation of Bacillariophyceae in Imo River,

Oyigbo  in relation to Months and Stations                                                    36

 

4.5       Mean monthly variation of Protozoa in Imo River in relation

to Months and Station                                                                                                41

 

4.6       Mean monthly variation of Rotifers in Imo River,

Oyigbo in relation to Months and Stations                                                     42

 

4.7       Mean monthly variation of Cladocera in Imo River,

Oyigbo in relation to Months and Stations                                                     43

 

 

 

 

 

 

 


 

CHAPTER ONE

INTRODUCTION


1.1 BACKGROUND TO THE STUDY

The phytoplankton constitutes the most important component of the food chain in every water body. All other living organisms in water depend directly or indirectly on them for food. A knowledge of the plankton community of any water body is therefore not only important in assessing its productivity but would permit a better understanding of the population dynamics and life cycles of the fish community (Abohweyere1990; Ugwumba 1990).

 

Estuarine plankton communities can respond to both climatic change and human activities at different time scale. There are obvious relationships between changes in plankton communities and water environmental factors. Hence, plankton may serve as a bio-indicator to monitor estuarine environment for both pollution or as a modeling for fish population dynamics (Nwankwo, 2004; Onyema, 2007).  According to Akomeah et al.( 2010), plankton are usually categorized according to their feeding mode or life cycle thus, 1) feeding mode: Phytoplankton = autotrophs, Zooplankton = heterotrophs, or 2) life cycle: holoplankton (entire life cycle in water column as plankton), meroplankton (part of life cycle as plankton). The amount of phytoplankton in water depends on light availability, the amount of nutrients available, and the relative proportions of nutrients available (with nitrogen and phosphorous usually as limiting nutrients) and the temperature of the water. The amount of zooplankton in water depends generally on the amount of phytoplankton and detritus available to feed on, detritus can be food for primary consumers (Hassan et al., 2010).  

The abattoir industry is an important component of the livestock industry providing domestic meat supply to over 150 million people and employment opportunities for teaming population in Nigeria (Nafarnda et al, 2012). However, majority of the activities going on in most of the abattoir present in Nigeria are never monitored for regulation purposes. Majority of the abattoir in Nigeria are not developed and facilities for the treatment of abattoir effluents are lacking. Potential health risks from waterborne pathogens can exist in water polluted by abattoir effluents, runoff from feedlots, dairy farms, grazed pastures, fallow and sod amended with poultry litter, grassland treated with dairy manure, and sewage sludge treated land. Such contamination of water bodies from abattoir wastes could constitute significant environmental and public health hazards (Nafarnda et al, 2012). These species among others include Staphylococcus sp., Streptococcus sp., in harsh environmental condition depicting the unfriendly nature of untreated abattoir effluent to the surrounding environment and human health (Coker et al., 2001). The main wastes originate from killing, hide removal or de-hairing, paunch handling, rendering, trimming, processing and clean-up operations. The wastes generated from slaughter house usually constitute blood, grease, inorganic and organic solids, salts and chemical added during processing operations (Adelegan, 2002). On the average, majority of the abattoir in Nigeria are sited near a stream, natural pond or river, like the one present in Imo River, Oyigbo, Rivers State.

 

Environmental issues resulting from improper management practices responsible for the pollution of the aquatic environment with various forms of contaminant has increased in geometric proportion over the last four decades with concomitant increase in water borne diseases especially typhoid, diarrhea and dysentery. Abattoirs are generally known all over the world to pollute the environment either directly or indirectly from their various processes. It is observed that in Nigeria, many abattoirs dispose their effluents directly into streams and rivers without any form of pre-treatment and the slaughtered meat is washed by the same water (Adelegan, 2002).

 

The Oyigbo abattoir on daily basis produces several gallons of blood, and intestinal contents and tissues introducing them into the environment including the Imo River. On a visit to the Oyigbo abattoir, during a quick survey around the abattoir, neither holding tank nor waste treatment system/facility was observed which is obviously an indication that effluent and other solid wastes generated from the abattoir are been dumped/discharged indiscriminately into the surrounding. The abattoir was said to have been in existence for years now, which implies that the water body has been receiving abattoir effluent for years. Many government agencies are interested in the interaction between water use and water pollution. Nevertheless, an increasing number of environmental protection agencies are being assigned the task of safe guarding the quality of water for multipurpose uses. It was recommended that all immediate and potential interests in the water basins are considered simultaneously in the management of water for all purposes. It has been observed that only an ecologically healthy fresh water ecosystem fulfills this global order and this can be measured by the ecology of the plankton in the water body.

 

Water used by man ranges from purely social needs such as recreation, religious worship and regional/cultural uses such as drinking, cooking, laundry, bathing, waste disposal, to economic needs such as irrigation, fisheries, animal production, electric power generation and navigation. For most of these uses, man depends mainly on fresh water available in inland lakes and rivers, which constitute less than 50% of the total amount of water in the biosphere (Yakubu et al., 2000). 


1.2       STATEMENT OF THE RESEARCH PROBLEM

 

Abattoirs are generally known all over the world to pollute the environment either directly or indirectly from their various processes hence the knowledge of  Plankton abundance and distribution is essential to the Imo River so as to ascertain the level of impact of the abattoir effluent periodically discharged to the river. Considering implicated certain bacteria species in abattoir effluent pollution, not ignoring other processes involved in meat and hide processing, the analysis deal with certain physiochemical and biological parameter of the river water.

 

Cases of water borne diseases especially typhoid, diarrhea and dysentery has resulted as a result of contact with polluted water. It thus becomes necessary to carry out such analysis on the river for awareness purpose, as the users of the river exploit it for economic and recreational purpose without the full knowledge of the danger it may pose to human health.

 

Until now in Nigeria, many abattoirs (Oyigbo Slaughter house included) dispose their effluents indiscriminately into streams and river body and without any form of treatment and mismanage various waste into the river.

 

1.4        JUSTIFICATION

I think one of the justification the study should be that despite the continuous discharge of abattoir waste from Oyigbo into Imo river for many years, no research have been carried out to ascertain the effect of such waste on the phytoplankton and aquatic health of the river, and/or that there is an observed plankton decline in the river, hence the need to ascertain the plankton decline and the cause of such decline in plankton abundance in the river.

 

1.4.    AIM AND OBJECTIVES OF STUDY

 

The study seeks to ascertain the effect of abattoir waste on Imo River at Oyigbo with specific objectives which were to:

 

1.      Check the effect of the abattoir waste on the abundance and distribution of plankton.   

2.      Check the physiochemical and biological properties of the river (if it influences any change in the DO, pH, Temperature). 

3.      Check for the level of impact if there is any, and

4.      Create awareness on various adverse impacts that may result from the discharge of untreated abattoir effluent.

 

1.5       SIGNIFICANCE OF THE STUDY

 

  1. The set of interest group that will benefit from the findings of this research will be the community and society where the abattoir is located, as the study stands to keep them informed.
  2. The study will be of great benefit to a number of groups in Nigeria. Government agencies especially the Rivers State Environmental Protection Agency, rank amongst the top beneficiary of this study
  3. The last set of beneficiary of this study are the researchers, academia, scientists and ecologist

 

1.6.         SCOPE AND LIMITATIONS

The area of this study covers a section of the Imo River around the effluent discharge point. The study is specifically focused on plankton abundance and distribution around the discharge point as defined by some physiochemical and biological parameters.

The research is not without limitation as there are bound to continuous flow of the river and other activities.



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