DURATION OF FERMENTATION ON THE NUTRITIVE VALUE OF WATER MELON SEEDS AND SENSORY EVALUATION OF SOUP MADE WITH IT

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ABSTRACT

Duration of fermentation on the nutritive value of watermelon seed and sensory evaluation of soup made with it. Watermelon seeds were fermented for 24-120hours (1-5 days) and sub-samples were collected daily and analyzed for proximate composition. Results show optimum increase in nutrients on 96hours fermented seed and this was used as a condiment along side with commercial ogiri from Africa oil bean for oha soup preparation both of which were subjected to sensory evaluation of attributes. Generally, findings show that the nutrient value of the seed increased significantly (P<0.05). The protein increased from 11.79% in the fresh sample to a maximum of 13.77% to (96hours fermented seed) while the ash increased from 4.95% to 5.75% in the same sample. Anti-nutrient analysis showed a decrease of tannin from 0.35% in the fresh seed to 0.04% in the 96hours fermented seed, while phytate reduces from 0.43% to 0.12% in the same sample and saponin reduced from 0.24% to 0.06%. The comparative assessing of the nutritive value of the fermented watermelon seed and commercial ogiri showed that the fermented seeds had higher protein content, 13.77% than the ogiri (9.98%) as well have higher fat content (15.40% while ogiri is reduce in fat 7.96%. The ogiri however had higher ash content (7.05%) and fibre content (2.87%) than the fermented watermelon seed which had 5.70% and 2.50% of ash and fibre respectively. Moisture and carbohydrate were also higher in the “ogiri” at 35.98% and 36.10% as against 33.1% and 29.40% which is recorded for the fermented seeds. The results sensory evaluation showed a higher preference for the fermented seed soup over ogiri soup. The fermented seed soup sco-for acceptability while the ogiri soup has 85.3% acceptability with a score of 7.68 on the same scale. The use of fermented watermelon seed as condiment for soups and stews was recommended.







TABLE OF CONTENTS

 

Title page                                                                                                                                i

Approval Page                                                                                                                        ii

Certification                                                                                                                           iii

Dedication                                                                                                                              iv

Acknowledgement                                                                                                                  v

Table of contents                                                                                                                    vi

List of tables                                                                                                                           vii

Abstract                                                                                                                                  viii

 

CHAPTER ONE

1.0           Introduction                                                                                                                1

1.1       Background of the study                                                                                             1

1.2       Statement of Problem                                                                                                 4

1.3       Objective of the Study                                                                                                5

1.4       Research Questions                                                                                                    5

1.5       Significance of the Study                                                                                           5

1.6       Scope of Study                                                                                                            6

 

CHAPTER TWO

2.0           The Literature Related                                                                                                7

2.1       Conceptual Framework                                                                                              7

2.1.1    Origin of Watermelon                                                                                                8

2.2       Uses of Watermelon Seeds                                                                                         11

2.3       Nutritional Benefits of Watermelon Seed                                                                  13

2.3.1    Protein Content                                                                                                          13

2.3.2    Vitamin A and C                                                                                                         13

2.3.3    B Vitamins                                                                                                                  14

2.3.4    Minerals                                                                                                                      14

2.3.5    Fat                                                                                                                               15

2.3.6    Calories                                                                                                                       15

2.4       Health Benefit of Watermelon Seed                                                                           15

2.4.1    Watermelon Seed and Hypertension                                                                          15

2.4.2    Heart Care                                                                                                                   15

2.4.3    Muscular Degeneration                                                                                              16

2.4.4    Kidney Disorder                                                                                                         16

2.4.5    Hydration                                                                                                                    16

2.5       Selection and Storage of Watermelon                                                                                    16

2.6       Watermelon Allergies                                                                                                17

2.6.1    Symptoms                                                                                                                   18

2.6.2    Diagnosis                                                                                                                    19

2.6.3    Causes of Watermelon Allergies                                                                                19

2.6.4    Avoidance                                                                                                                   19

2.7       Fermentation                                                                                                              20

2.7.1    Types of Fermentation                                                                                               21

2.8       Principle of Fermentation                                                                                           22

2.8.1    Bacteria                                                                                                                      22

2.8.2    Yeast                                                                                                                           23

2.8.3    Moulds                                                                                                                        23

2.8.4    Enzymes                                                                                                                     24

2.9       Ogiri                                                                                                                            24

2.10     Types of Ogiri                                                                                                            25

2.11     Nutritional Benefit of Ogiri                                                                                        25

2.12     Uses of Ogiri                                                                                                              26

2.13     Processing of Ogiri                                                                                                     26

2.2       Theoretical Frameworks                                                                                             27

2.2.1      Theories Of Innovation                                                                                              27

2.2.2      Operational Theory                                                                                                     28

2.2.3      Social Factors                                                                                                             29

2.3         Theories on Sensory Properties                                                                          30

2.4       Related Empirical Study                                                                                             30

2.5       Summary of Literature Reviews                                                                                 32

 

CHAPTER THREE

3.0       Materials and Method                                                                                                 34

3.1        Area of the Study                                                                                                  34

3.2       Design of the Study                                                                                                    34

3.3        Sources of Material                                                                                              34

3.4       Population of the Study                                                                                              34

3.5       Instrument for Data Collection                                                                                   35

3.6       Data Collection Techniques                                                                                       35

3.6.1    Determination of Moisture Content                                                                           35

3.6.2    Ash Content Determination                                                                                       36

3.6.3    Protein Determination                                                                                                36

3.6.4    Crude Fibre Determination                                                                                        37

3.6.5    Fat Content Determination                                                                                         38

3.6.6    Carbohydrate Content Determination                                                                        39

3.7       Determination of Phosphorus                                                                                     39

3.7.1    Determination of Calcium and Magnesium                                                               40

3.8       Fermentation of Watermelon Seed                                                                             40

3.9       Determination of Anti-nutrients                                                                                 41

3.9.1    Tannin Determination                                                                                                42

3.9.2    Saponin Determination                                                                                              43

3.10     Recipe for the Soups                                                                                                   43

3.10.1  Fermented Watermelon Seeds (Ogiri                                                                         44

3.10.2  Preparation of the Fermented Watermelon Seed Soup and Ogiri Soup                44

3.11     Sensory Evaluation                                                                                                                             45

3.12     Data Analysis Techniques                                                                                          45

 

CHAPTER FOUR

4.0       Results and Discussions                                                                                              46

 

CHAPTER FIVE

5.0       Summary, Conclusion and Recommendation                                                            52

5.1       Summary                                                                                                                    52

5.1.1    Restatement of Problem                                                                                             53

5.2       Conclusion                                                                                                                  53

5.3       Recommendation                                                                                                       53

Appendix 1: Sensory Analysis Questionnaires

Appendix 2: Raw and Dehulled Water Melon Seeds

Appendix 3: Fermented Water Melon Seeds Duration Time

Appendix 4: Soup Samples Produce with Fermented Water Melon Seed and Ogiri

 

 

 

 

 

 

LIST OF TABLES


Table 1: Proximate Composition of fermented Watermelon Seed for 24 -120 hrs. (%)

Table 2: Anti-nutrient content of raw and fermented watermelon seed (%)

Table 3:  Proximate composition of fermented watermelon seed (96hrs) and commercial ogiri

Table 4: Mean scores of sensory evaluation of fermented watermelon seed and commercial ogiri

 

 

 

 

 

 

 

 

CHAPTER ONE

INTRODUCTION


            1.1           BACKGROUND OF THE STUDY

Watermelon is a tropical fruit which grows in almost all part of Africa and South East Asia (Koocheki, 2007). It belongs to the family of cucumber (cucurbitacca). It is large, oval, round or oblong in shape. The skin is smooth with dark green rind or sometimes pale green strips that turn yellowish green when ripe (Gwana, 2011) watermelon was described by Roger (2006) as citrullus lanatus and is reported to contribute to the proper functioning of the kidney.

Watermelon is a type of melon, member of the gourd family, cultivated extensively for its pleasant tasting fruit is one of the most economically important fruit in the cucurbitaceae family. Watermelon is reported to be rich in water content with high sugar for energy boosting as well as for its rich in mineral and vitamin (Aidoo, 2004) watermelon grows as a trailing vine.

Watermelon are consumed fresh, leading to the rejection of watermelons that have any visible defect (Williams, 2007). Watermelon serves as a good source of phytochemical and lycopene, a red carotenoid pigment which acts as antioxidant during normal metabolism and protects against cancer (Perkins, 2004). Watermelon is rich in carotenoid some of which include lycopene, phytofluene, phytoene, beta-carotene lutein-lycopene make up the majority of the carotenoids in watermelon (Collins, 2005). Watermelon helps to regulate acid – base equilibrium which lowers the cholesterol level, which has strong diuretic tendencies (ie increases the amount of water in the urine) remove excess water from the body, contribute to clearing the kidney or prevent the formation of bladder stones, kidney stones among other (Erukainure, 2010). Watermelon contains 96% water, and vitamin C and traces of cholesterol, watermelon also contains thirst quencher and also some anti-inflammatory compounds responsible for asthma, atherosclerosis, diseases, diabetes, colon cancer and arthritis (Sundia, 2007) watermelon is an important source of potassium and many micronutrients (Kondale, 2002). The potassium and magnesium present in watermelon helps in reducing blood pressure, the carotenes present in them assist greatly in preventing hardening of walls of arteries and vein thereby helping in that regard (Dike, 2011). Watermelon is effective in reducing one blood pressure many people in the tropical region eat the fruit daily in the afternoon to protect themselves from heat burn, it also help in proper functioning of insulin in the body thus lowering the blood sugar level (Kondale, 2003).

Watermelon is used amazingly for it nutritional and medicinal value because of its high water content which contain sugar and energy booster, which hydrate body in the case of dehydration, especially during the hot season. In Nigeria many types of watermelon are cultivated especially in the Northern region, but the consumption of watermelon in nationwide, not much has been recorded on the utilization of watermelon seeds partly in Nigeria (Onifiole, 2002). However, report has show that the seeds are consumed in different ways as snacks in Asia as well as utilized as significantly in livestock feeds (Achinewhu, 2003) watermelon seeds are flat having marginal groove on each side near the base and white black margins 10-15mm long (Alexander, 2003). Watermelon seeds are rich in macro and micro nutrients such as magnesium, calcium, potassium, iron, phosphorus and zinc etc. which assist in the growth and development of the healthy body which take part in metabolic activities of all living organisms. (Adams,2012). Watermelon seeds are excellent sources of protein it contains phytonutrients which have very good on the health and proper functioning of internal organs (Perkins, 2006).

Watermelon seed contain many beneficial minerals like phosphorus (mg /100,705-755g) potassium (648-689mg/1100g) calcium (54-116mg/100g) sodium (2\3-99mg/100g) iron (677-720\8mg/100g) and copper (069-175mg/100g) (Egan, 2001). Watermelon seed are rich in good fats and proteins, it contain phytonutrient which very good effect in the health. Watermelon seeds are used in making local beads (Pegg, 2007). Most American price the sweet and juicy fresh of watermelon, but remove or spit out the seeds has led to the development of “seedless” watermelon which produce much smaller seeds are consumed in many cultures around the world, because they are relatively rich source of certain nutrient (Vranizan, 2005).

Watermelon seeds comprises of protein, vitamin B, minerals, fat, calories, and fat content (Ainsley, 2010). Many nutrients are beneficial for our body. Health nutrients in watermelon seeds are able to ward off cancer improve or prevent cardiovascular disease, hypertension and reduces level of bad cholesterol (Ensmiger, 2003). However, in order to create more report on the importance of usually discarded watermelon seed, it is necessary to assess the quality of watermelon seed. With a view of harnessing them for consumption and possible industry usage (Elemo, 2002).

Ogiri is a fermented food condiment of wide application and use in Nigerian cuisines (Odunfa, 2005). Ogiri is an oily paste produced mainly from melon seeds and consumed widely within the West Africa. It is a cheap soup condiment among the rural dwellers (Achi, 2005). Many different seeds have been used successfully in the production of ogiri using chance inoculated microorganisms to effect fermentation (Oyemuga, 2003). In the South East, the Igbo’s use seeds of the castor plant, (Rianus comminis) for ogiri production (Enugingha, 2003) as well as fermented pumpkin, (Telferia Oceifentalis) for the same purpose (omafuvbe, 2000). David and Ademibigbe (2010) observed the existence of many different varieties of melon seeds (other than the popular Egusi colanatus) which are correctly underutilized and which world same as alternative to egusi in the production of ogiri, the popular widely consumed condiment for soups and stews. Watermelon seed, readily fits into the same picture for possible use in the production of ogiri when reported fermented (Asagbra, 2009).seeds of watermelon has been reported to be rich in protein, minerals and vitamins as well as contain a wide variety of phytochemicals stone of which have been shown to posses pharmacological and other health benefits. (Adewusi, 2004). The practice of throwing watermenlon seeds away during the fruit consumption is common in the South East Nigeria, (Odunfa, 2002). A successful utilization of the seeds in production of consumables will no donst unincrease and durasity its utility value. (Campbell, 2000). Against this background, this popular work was designed to study the duration of fermentation on the nutritive value of the watermelon seed a view to establishing an optimum fermentation time which will be utilized in the production of ogiri and subsequent determination for the acceptability of the ogiri produced through sensory evaluation. (Eka, 2010).


            1.2           STATEMENT OF THE PROBLEM

Watermelon is one of the fruit that is now found all through the year in the market in all the four cardinal points in Nigeria. Many people throw the watermelon seeds as waste. Recent research has shown that watermelon seeds contain protein, mineral and vitamins. In the South East, the Igbo’s  use seeds of the castor plant for ogiri in cooking of soup and stews due to the flavor and its cheap rate in the society. Watermelon seed readily fits into the same picture for possible use in the production of ogiri when fermented which serve as a condiment for our local cuisines. The practice of throwing watermelon seeds away during the fruit consumption is common in the South East Nigeria. A successful utilization of the seeds in the production of consumables will unincrease and durasity its utility value.

 

            1.3           OBJECTIVE OF THE STUDY

The main objective of this study was to determine the duration of fermentation on the nutritive value of watermelon seeds and sensory evaluation of soup made with its.  Specifically the study:

1.     Determined the nutritional content of the fermented watermelon seed in relation to the length of fermentation.

2.     Determined the optimum fermentation period that has the positive effect on the nutrients of watermelon seeds.

3.     Accessed the level of acceptability of soup cooked with the fermented watermelon seed as a condiment through sensory evaluation by unbiased panelists.


1.4       RESEARCH QUESTION

1.     What are the nutritional contents of the fermented watermelon seed in relation to the length of fermentation?

2.     What is the optimum fermentation period that has the positive effect on the nutrients of watermelon seeds?

3.     How do people accept the soup cooked with fermented watermelon seed as a condiment?


1.5       SIGNIFICANCE OF THE STUDY

This study will be beneficial to individual home maker, eatery, restaurant, hostel and food industries.

Individual will benefit from this study which will help them in enriching their food using fermented watermelon seeds as a condiment because of the nutrient that is embedded in the seed.

This study will help the homemaker to adopt a new soup style of fermented watermelon seeds as a condiment and include it in their food menu which can serve as a recipe for different food product which help in improving the health of human beings because of it various valuable nutrients which it contains.

A homemaker can also benefit from the seeds by using the seed in the snack production for her family.

Food industries will benefit a great deal from using this seed as a condiment in a soup because its varieties will come into their menu list.

Acknowledgement of watermelon seed and it fruit help to improve their recipe in different food preparation it can also help them in giving their clientele varieties by adopting different styles of soup made with fermented watermelon seed as condiment which will enhance the people health wise because of its nutritive value which it contains.


1.6       SCOPE OF THE STUDY

The study is limited to the duration of fermentation on the nutritive value of watermelon seed and sensory evaluation of soup made with it which has the highest nutritive value.

 

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