PRODUCTION AND EVALUATION OF CASSAVA WHEAT BREAD FROM FIVE IMPROVED CASSAVA VARIETIES

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ABSTRACT

Cassava (Manihot esculenta Crantz) varieties: TME 4779, TME 419, NR 8082, TMS 30572 and TMS 93604 gotten from Enugu state, were processed into flour. Comparative studies of their physicochemical and organoleptic properties were evaluated. Anti-nutrient (HCN) composition was determined on the cassava roots, functional properties were carried out on the cassava flour, proximate, mineral and sensory attributes of the cassava flour and bread were also analyzed. Atomic spectroscopy, extraction, kjeldahl methods were used to determine the mineral and proximate properties of cassava flours and bread respectively. Functional properties were determined by physical, chemical and organoleptic assessment of the flour samples while sensory analysis of the cassava flour varieties and cassava-wheat bread was carried out by semi-trained panelists using the 9-point hedonic scale. Results obtained showed that the moisture, crude fiber, ash content and carbohydrate content of the five cassava-wheat bread differ significantly (p≤0.05) as they ranged from 26.00-31.05, 1.48-1.69, 2.37-2.67, 44.01-50.55 respectively while for crude protein, the values were not significantly different (p≥0.05) as they ranged from 12.66-13.99.  Also, the swelling index, oil absorption capacity, emulsification capacity and water absorption capacity of each sample was significantly (p≤0.05) different. The combination levels for bread containing wheat and cassava varieties of 10% and 20% respectively was found to be acceptable. It was found that the carbohydrate content of cassava-wheat bread of 20% TMS 93604 was higher than the other cassava flour samples. Breads baked with 80% wheat flour and 20% cassava flour were less accepted by the sensory panel in terms of appearance, taste, texture and aroma. Sample 30572A (90: 10) wheat to 10% TMS 30572 cassava flour which had the greatest value for most essential physicochemical properties and hence is a good substitute for 100% wheat flour bread. These results showed that cassava flour has potential to replace part of the wheat flour in the production of bread since there is wide acceptance amongst the judges.




TABLE OF CONTENTS

Title page        i
Declaration        ii
Certification        iii
Dedication        iv
Acknowledgments        v
Table of Contents        vi
List of Tables        x
List of Figures        xi
List of Plates        xii
Abstract        xiii

CHAPTER 1: INTRODUCTION
1.1       Introduction                  1
1.2       Statement of the Problem                    3
1.3       Justification of the Study        3
1.4       Objectives of the Study                    3

CHAPTER 2: LITERATURE REVIEW
2.1       Bread                                5
2.2       Wheat Imports and Impact on Foreign Currency Earning     7
2.3 Composite Flour in Bread making                9
2.4 Determination of the quantity of Composite Flour in Bread making        11
2.5    Cassava        13
2.6    Composition of Cassava Root        15
2.7 Cassava Flour in baked food        16
2.8 Nutritional Value of Cassava Roots        18

CHAPTER 3: MATERIALS AND METHODS
3.1       Source of Raw Materials        20
3.2       Sample Preparation        20
3.2.1    Production of Cassava Flour                    20
3.3       Formulation of Composite Flour        22
3.4       Production of Bread                                                               23
3.5       Determination of Functional Properties of Flour          25
3.5.1 Bulk Density        25
3.5.2 Water Absorption Capacity        25
3.5.3 Oil Absorption Capacity (OAC)        25
3.5.4 Gelation Temperature        26
3.6       Functional Properties of Flour        23
3.5.5    Swelling Index        26
3.5.6    Determination of Emulsion Activity and Stability    27
3.6    Proximate Analysis of Cassava Flour and Bread    27
3.6.1    Determination of Moisture Content       27
3.6.2    Determination of Ash Content        28
3.6.3 Determination of Fat Content        28
3.6.4 Determination of Crude Fibre        29
3.6.5 Determination Crude Protein        29
3.6.6 Determination of Carbohydrate Content     30
3.7 Physical Analysis of Bread        30
3.7.1 Determination of Bread Weight        30
3.7.2 Determination of Loaf Volume        30
3.7.3 Determination of Specific Volume        31
3.7.4 Determination of Oven Spring        31
3.8 Mineral Analysis of Cassava Flour and Bread      31
3.8.1 Determination of Magnesium        31
3.8.2 Determination of Phosphorus        32
3.8.3 Determination of Potassium        33
3.8.4 Determination of Sodium        33
3.8.5 Determination of Calcium Propionate      34
3.9 Determination of Hydrogen Cyanide Analysis of the Cassava Root        34
3.10 Sensory Evaluation of Cassava Flour and Bread     35
3.11 Experimental Design        35
3.12 Statistical Analysis        35

CHAPTER 4: RESULTS AND DISCUSSION
4.1 Functional Properties of Cassava Flour        37
4.2 Proximate Composition of the Cassava Flour and Bread    40
4.2.1   Proximate Properties of the Cassava Flour Samples   40
4.2.2   Proximate Properties of Bread Loaves       42
4.3 Physical Properties of Bread Loaves        45
4.4 Mineral Evaluation of Cassava Flour and Bread     48
4.4.1 Mineral Composition of Cassava Flour        48
4.4.2 Mineral Composition of Cassava-Wheat Bread       50
4.5      Anti-Nutritional Factors of Cassava Roots   53
4.6      Sensory Attributes of the Flour and Bread Samples        55
4.6.1   Sensory Evaluation of the Cassava Flour       55
4.6.2   Sensory Evaluation of Cassava-Wheat Bread   58

CHAPTER 5: CONCLUSION AND RECOMMENDATIONS
5.1 Conclusion        60
5.2 Recommendations        60
REFERENCES                     62           







LIST OF TABLES

Table 2.1: Proximate Composition of Fresh Cassava Root 16

Table 3.1: Flour Blends Formulation 22

Table 3.2   Recipe for Production of Bread 23

Table 4.1   Functional Properties of Cassava Flour 38

Table 4.2.   Proximate properties of the Cassava Flour Samples 41

Table 4.2.1 Proximate Properties of Bread Loaves 43

Table 4.3 Physical Properties of Bread Loaves 47

Table 4.4 Mineral Content of Cassava Flour 49

Table 4.4.1 Mineral composition of Cassava –Wheat Bread 52

Table 4.5 Anti-nutritional Factors of Cassava Root 54

Table 4.6. Sensory Evaluation of Cassava Flour 57

Table 4.6.1. Sensory Evaluation of Cassava- Wheat Flour Bread 59







LIST OF FIGURES

Figure 3.1: Flow chart for Processing Cassava Flour 21 

Figure 3.2: Flow chart for Bread Production 24






LIST OF PLATES

Plate 1: Unpeeled Cassava Roots             23

Plate 2: Peeled Cassava Roots 23

Plate 3: Moulded Cassava Dough             23

Plate 4: Cassava Bread Sample 23






CHAPTER 1

1.1 INTRODUCTION
In many developing countries such as Nigeria, bread consumption is expanding and there is an increasing dependence on imported wheat which depletes the country's foreign exchange (Dewettnick et al., 2008). Bread can be described as a fermented confectionary product made mainly from wheat flour, water, yeast and salt by series of process involving mixing, kneading, proofing, shaping and baking. It is an important staple food in both developing and developed countries and constitutes one of the major sources of nutrients such as carbohydrates, protein, fibre, vitamins and minerals in the diet of many people worldwide (Ogunjobi et al., 2010). The consumption of bread in Nigeria is on a steady increase because it is convenient and ready to eat food normally consumed as breakfast and sometimes lunch. It has become the second most widely consumed non - indigenous food after rice (Siddiq et al., 2009). 

Milligan  et  al.,  (2010)  defined  composite  flour as a mixture of flours, starches and other ingredients intended  to replace  wheat  flour  totally or  partially in  bakery  and  pastry  products.  Shittu  et  al.,  (2007) also  stated  that  as the  composite flours  used were either binary or ternary mixtures of flours from some  other  crops with  or  without  wheat flour. The use  of  composite  flours  had  a  few  advantages  for developing  countries  such  as  Nigeria  in  terms  of: i) the saving of hard currency; ii) promotion of high-yielding, native plant species; iii)  a  better  supply  of protein for human nutrition; and iv) better overall use of domestic agriculture production (Bugusu et al., 2001).  Composite  flour is considered advantageous  in  developing countries  as  it  reduces the  importation  of wheat  flour  and  encourages  the use  of  locally  grown  crops  as  flour  (Hasmadi  et  al.,  2014).  Local raw materials substitution for wheat flour is increasing due to the growing market for confectioneries (Noor and Komathi, 2009). Thus, several developing countries have encouraged the initiation of programmes to evaluate the feasibility of alternative locally available flours as a substitute for wheat flour (Abdelghafor et al., 2011). Moreover, the  concept of composite  technology initiated by the Food and Health Organization (FAO) in  1964  was  targeted at  reducing  the  cost  of  support for  temperate  countries  by  encouraging  the  use  of indigenous  crops  such  as cassava,  yam, maize  and others  in  partial  substitution  of wheat  flour (Satin, 1988).  The FAO reported  that  the  application  of composite flour  in various food  products  would be economically  advantageous  if  the imports  of  wheat could be reduced or even eliminated, and that demand for  bread  and pastry  products  could  be met  by  the use of domestically grown products instead of wheat (Jisha  et  al.,  2008).  The bakery  products  produced using  composite  flour  were  of  good  quality,  with some  characteristics  similar  to  wheat-flour  bread, though the texture and the properties of the composite flour bakery products were different from those made from wheat flour, with an increased nutritional value and the appearance. Apart from being a good source of  calories  and  other nutrients,  wheat  is  considered nutritionally poor, as cereal proteins are deficient in essential  amino  acids  such  as  lysine  and  threonine (Dhingra and Jood, 2001). Therefore, supplementation of wheat flour with inexpensive  staples,  such  as cereals  and  pulses,  helps  improve  the  nutritional quality of wheat products (Sharma et al., 1999). For example, the protein quality of both the cassava-soya and the cassava-groundnut breads is higher than that of common wheat bread (Nilufer et al., 2008).

1.2 STATEMENT OF THE PROBLEM
Most of the studies conducted on the use of composite flour for bread making purposes (Dhingra et al., 2005;  Adeyemi et al., 2007;) were devoted to determining the effect of biological origin of flour and level of wheat flour substitution on their bread making quality while Shittu et al., (2006) were concerned with the effect of baking time and temperature on some physical properties of the cassava-wheat bread loaf but very few detailed studies concerning the nutritional evaluation of bread loaves produced from cassava wheat flour have been performed till now. Hence, the aim of this research was to assess the anti-nutrient, physical, chemical and nutritional properties of the cassava tubers, flour and bread respectively.

1.3 JUSTIFICATION OF THE STUDY
Cassava which has become one of the largest cultivated food products has been found to be a very good source of energy as it contains carbohydrate, dietary fibre, minerals, vitamins and ash. It is also a readily available food product and it is affordable. An inclusion of this essential raw material in bread production will aid in reducing the cost of production of bread and also provide a richer bread product containing essential nutrients.

1.4 OBJECTIVE OF THE STUDY
The main objective of this work was to identify and produce cassava-wheat flour bread using five improved varieties of cassava (TME 4779, TME 419, TMS 30572, NR 8082 and TMS 93604) from Enugu state.

The specific objectives were to;

i. obtain some improved cassava varieties from Enugu state.

ii. produce cassava flour from the roots and evaluate functional composition of the flour.

iii. produce bread from the cassava-wheat flour.

iv. evaluate physical and sensory properties of the bread produced.

v. evaluate proximate, mineral and hydrogen cyanide contents of the root, flour and bread.


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