THE OXIDATIVE STRESS STATUS OF RATS FED ON OIL BEAN SEED MEAL

  • 0 Review(s)

Product Category: Projects

Product Code: 00002864

No of Pages: 51

No of Chapters: 5

File Format: Microsoft Word

Price :

₦3000

  • $

ABSTRACT

 

This study was on oxidative stress status of rat fed with Pentraclethra macrophylla, otherwise known as African oil bean seed oil in English or Ugba in Igbo. Sixteen male rats were distributed into four groups. 1 (control), then group 2, 3, and 4 as test groups. They were fed with their formulated meal (5%, 10%, 20% inclussions) for 28 days. Group 1 was the control and were fed with the normal feed, while group 2, 3 and 4 which were the test groups were fed with test feed formula.. The parameters determined were MDA concentrations and catalase activity. Serum MDA significantly increased (p<0.05) while the catalase activity significantly decreased (p<0.05). This finding may be clinically significant to individuals with predisposition to increased generation of reactive oxygen species (ROS) and other degenerative diseases.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

TABLE OF CONTENT

 

Title page

 

Approval page

Dedication

 

Acknowledgment

 

Abstract

 

Table of content

 

Chapter One

 

1.0 Introduction

 

1.1 Aim and objective

 

Chapter Two

 

2.0 Literature Review

 

2.1 Classification of oil bean

 

2.2 Composition of seed

 

2.3 Fatty acid composition of African oil bean seed

 

2.4 Pharmacological uses

 

2.5 Anti nutrient in health

 

2.6 Oil bean and humans

 

2.6.1 Oil bean seed and animals

 

2.7 Oxidative stress

 

2.7.1 Malondialdehyde

2.8 Catalase

Chapter Three

 

3.0 Materials and methods

 

3.1 Materials

 

3.2 Collection and identification of plant materials

 

3.3 Animals

 

3.4 Preparation of oil bean seed meal for animal feeding

 

3.4 .1 Oil bean seed meal inclusion diet preparations

 

3.4.2 Oil extraction

 

3.5 Formulation of oil bean seed meal diet

 

3.5.1 Oil bean seed based treatment diet (g/100g Diet)

 

3.5.2 Chemicals/Biochemicals

 

3.6 Phytochemical screening

 

3.6.1 Test for tannins

 

3.6.2 Test for alkaloids

 

3.6.3 Test for saponin

 

3.6.4 Test for flavonoids

 

3.6.5 Determination of phenols

 

3.7 Determination of fatty acid composition

 

3.7.1 Determination of anthocyanin

 

3.8 Collection of blood sample


3.9 Test for malonaldelyde

 

3.9.1 Catalase assay

 

Chapter Four

 

4.0 Result and discussion

 

4.1 Figure i fatty acid composition of extracted oil of  pentraclethra macrophylla

 

32

 

4.2 Figure ii malondialdehyde concentration of test and control animal

 

4.3 Figure iii catalase concentration of test and control animals

 

Chapter Five

 

5.0 Discussion

 

5.1 Recommendations

 

References

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CHAPTER ONE

 

 

THE OXIDATIVE STRESS STATUS OF RATS FED ON OIL BEAN

 

 

SEED MEAL

1.0.         INTRODUCTION

 

Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Igbo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth).

 

The natural fermentation of the seed which at present is still done at the house-hold level, renders the production nutritious, palatable and non-toxic (Enujiugha, 2002).

 

Its production, like many African fermented foods depends, entirely on mixed fermentation by microorganism from diverse source.

 

Pentaclethra macrophylla Benth is a large woody plant abundant in the rain forest areas of west and central Africa. It’s origin in Nigeria is believed to be around

 

1937 (Ladipo, 1984); where it is found in the South Nigeria, (Mbajunwa et al.,

 

1998).

 

 

―Ugba‖ Pentaclethra macrophylla Benth belongs to the Family Leguminosae and sub-family Microsoideae (Keay, 1989 and NFTA, 1995).

 

Ugba seeds are irregular and oval; they are flat, black and hard pods. It is composed of oil, protein and small amounts of carbohydrate (Obeta, 1982).

 

AIM AND OBJECTIVES

(i)                To determine the concentration of Malondialdehyde (MDA, which indicates the peroxidation status) and

 

(ii)             The activity of Catalase (a marker of antioxidant status) in the serum of rats fed graded doses of African oil bean seed meal

Click “DOWNLOAD NOW” below to get the complete Projects

FOR QUICK HELP CHAT WITH US NOW!

+(234) 0814 780 1594

Buyers has the right to create dispute within seven (7) days of purchase for 100% refund request when you experience issue with the file received. 

Dispute can only be created when you receive a corrupt file, a wrong file or irregularities in the table of contents and content of the file you received. 

ProjectShelve.com shall either provide the appropriate file within 48hrs or send refund excluding your bank transaction charges. Term and Conditions are applied.

Buyers are expected to confirm that the material you are paying for is available on our website ProjectShelve.com and you have selected the right material, you have also gone through the preliminary pages and it interests you before payment. DO NOT MAKE BANK PAYMENT IF YOUR TOPIC IS NOT ON THE WEBSITE.

In case of payment for a material not available on ProjectShelve.com, the management of ProjectShelve.com has the right to keep your money until you send a topic that is available on our website within 48 hours.

You cannot change topic after receiving material of the topic you ordered and paid for.

Ratings & Reviews

0.0

No Review Found.

Review


To Comment


Sold By

ProjectShelve

7891

Total Item

Reviews (29)

  • Anonymous

    1 week ago

    This is truly legit, thanks so much for not disappointing

  • Anonymous

    1 week ago

    I was so happy to helping me through my project topic thank you so much

  • Anonymous

    2 weeks ago

    Just got my material... thanks

  • Anonymous

    3 weeks ago

    Thank you for your reliability and swift service Order and delivery was within the blink of an eye.

  • Anonymous

    3 weeks ago

    It's actually good and it doesn't delay in sending. Thanks

  • Anonymous

    3 weeks ago

    I got the material without delay. The content too is okay

  • Anonymous

    1 month ago

    Thank you guys for the document, this will really go a long way for me. Kudos to project shelve👍

  • Anonymous

    1 month ago

    You guys have a great works here I m really glad to be one of your beneficiary hope for the best from you guys am pleased with the works and content writings it really good

  • Anonymous

    1 month ago

    Excellent user experience and project was delivered very quickly

  • Anonymous

    1 month ago

    The material is very good and worth the price being sold I really liked it 👍

  • Anonymous

    1 month ago

    Wow response was fast .. 👍 Thankyou

  • Anonymous

    1 month ago

    Trusted, faster and easy research platform.

  • TJ

    1 month ago

    great

  • Anonymous

    1 month ago

    My experience with projectselves. Com was a great one, i appreciate your prompt response and feedback. More grace

  • Anonymous

    1 month ago

    Sure plug ♥️♥️

  • Anonymous

    1 month ago

    Thanks I have received the documents Exactly what I ordered Fast and reliable

  • Anonymous

    1 month ago

    Wow this is amazing website with fast response and best projects topic I haven't seen before

  • Anonymous

    1 month ago

    Genuine site. I got all materials for my project swiftly immediately after my payment.

  • Anonymous

    1 month ago

    It agree, a useful piece

  • Anonymous

    2 months ago

    Good work and satisfactory

  • Anonymous

    2 months ago

    Good job

  • Anonymous

    2 months ago

    Fast response and reliable

  • Anonymous

    2 months ago

    Projects would've alot easier if everyone have an idea of excellence work going on here.

  • Anonymous

    2 months ago

    Very good 👍👍

  • Anonymous

    2 months ago

    Honestly, the material is top notch and precise. I love the work and I'll recommend project shelve anyday anytime

  • Anonymous

    2 months ago

    Well and quickly delivered

  • Anonymous

    2 months ago

    I am thoroughly impressed with Projectshelve.com! The project material was of outstanding quality, well-researched, and highly detailed. What amazed me most was their instant delivery to both my email and WhatsApp, ensuring I got what I needed immediately. Highly reliable and professional—I'll definitely recommend them to anyone seeking quality project materials!

  • Anonymous

    2 months ago

    Its amazing transacting with Projectshelve. They are sincere, got material delivered within few minutes in my email and whatsApp.

  • TJ

    4 months ago

    ProjectShelve is highly reliable. Got the project delivered instantly after payment. Quality of the work.also excellent. Thank you