EFFECT OF PACKAGING MATERIALS AND STORAGE CONDITIONS ON STABILITY OF SELECTED INDIGENOUS SOUPS OF SOUTH-SOUTH NIGERIA

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ABSTRACT

 

This study evaluated the effect of packaging materials and storage conditions on stability of three indigenous soups of South South Nigeria. The soups namely AfangEdikang Ikong and Afere Ndek Iyak soups were packaged in Plastic, Polyethyelene bags and Aluminum pouches and stored at ambient, refrigeration and freezing condition for five months. Soups were evaluated for quality changes monthly. Response Surface methodology was used to evaluate the performance of the packaging materials, the storage temperature and the soups. Some of the quality indices evaluated were the proximate composition, the vitamins and mineral elements. Others were the free fatty acids, thiobabituric acid, total soluble solids and microbial analysis. The soups in freezing and refrigeration storage were analysed monthly while those in ambient storage were analysed daily.  The soups stored at ambient temperature had microbial counts beyond acceptable limits (105cfu/g) after 24 hours, irrespective of whether it was packaged in plastic, polyethylene bag or aluminum pouch. The soups stored in refrigeration condition had significant (p< 0.05) microbial and chemical deterioration after two months storage and were discarded, while those in the Freezing storage lasted five months. The proximate composition of the soup showed that: Protein ranged from 4.32 to 5.77%, Fat 4.30 to 10.86%, Ash- 2.33 to 2.88, Moisture- 76.26 to 82.07%. The micronutrient composition of the soups was: Iron – 0.6 to 0.87 mg/100g, Calcium- 170.88 to 186.94mg, Vitamin B1- 0.26 to 0.77mg/100g, Vitamin C- 16.39 to 29.29 mg/100g. The physicochemical properties of the soup were thus:  Free Fatty Acid- 1.29 to 2.55%; Thiobiaturic Acid- 0.032 to 0.046%, Peroxide value- 1.785 to 2.880 mg/kg, pH- 6.33 to 6.69, Total Soluble Solids- 2.795 to 3.570%. The microbial properties indicated that Total Fungal Count (TFC) ranged from 1.75 x 103 to 6.0 x 103cfu/ml while Total Viable Count (TVC) ranged from 8.35 x 103 to 14.95 x 103cfu/ml. The shelf life was studied using physicochemical properties, all the soups had similar trend after storage for five months but optimization using Response Surface Methodology showed that Afere Ndek Iyak, Polyethylene bag and Freezing was the best.







TABLE OF CONTENTS

TITLE                                                                                                                             PAGES

Title Page                                                                                                                    i

Title Page                                                                                                                    ii

Declaration                                                                                                                  iii

Certification                                                                                                                iv

Dedication                                                                                                                  v

Acknowledgements                                                                                                    vi

Table of contents                                                                                                        vii

List of Table                                                                                                                xv

List of Figures                                                                                                             xvii

Abstract                                                                                                                      xviii

 

CHAPTER 1: INTRODUCTION

1.1       Background of the Study                                                                               1

1.2       Statement of Problem                                                                                     4

1.3       Justification of the Study                                                                               7

1.4       Aim and Objectives of the Study                                                                   8

 

CHAPTER 2: LITERATURE REVIEW

2.1    Soup: Meaning, Types And Use                                                                     9

2.2       Soups of Various Tribes And Ethnic Groups Of Nigeria                               10

2.3       Indigenous Soups of South South Nigeria                                                     12

2.4       Traditional Methods of Storing and Preserving Soups in South South Nigeria   13

2.5       Keeping Quality of Soups                                                                              14

2.6       Types Of Soup Spoilage                                                                                 15

2.7       Types and Characteristic Qualities of Different Packaging Materials            16

2.7.1    Tinplate                                                                                                           16

2.7.2    Aluminum                                                                                                       18

2.7.3    Glasses and pouches                                                                                       19

2.7.4    Packaging with nylon                                                                                     20

2.7.5    Papers, woods and other African indigenous forms of food packaging

and preservation                                                                                              21

2.8    African Indigenous Forms of Food Packaging And Preservation                 21

2.8 .1   Refrigeration and freezing storage (low temperature storage)                       23

2.8.2    Thermal (high temperature- cooking, sterilization) processing                       24

2.8.3    Use of synthetic preservatives                                                                        26

2.9       Characteristics of Packaging Materials (Pouches and Cans)                          27

2.10     Food Processed and Stored In Other Container In Ready To

 Eat Form (RTE)                                                                                             29

2.11     Storage Stability of Packaged Food Nutrients as a Function of Time           30

2.12     Packaging Materials: Plastics, Pouches and Nylon                                         30

2.13     Changes in the Physicochemical Characteristics of Soups, Sauces and Stew 31

 

2.14     Microorganisms in Food (Soup) Spoilage                                                       32

2.15     Effects of Freeze-Thaw Cycles on Soups Sauces and Stew                          34

2.16     How Freezing Affects the Quality of Foods                                                  34

2.16.1  Effect of freezing                                                                                          34

2.16.2  Factors affecting frozen food quality                                                              35

2.16.3  Effect of storage; the role of glass transition                                                  35

2.17     Afang Soup                                                                                                     36

2.18     Edikang Ikong Soup                                                                                       37

CHAPTER 3: MATERIALS AND METHODS

 3.1      Materials                                                                                                            38

3.2.      Preparation of Soups                                                                                           38

3.2.1    Preparation of afang soup                                                                                   38

3.2.2    Preparation of afere ndek iyak  soup                                                                   40

3.2.3    Preparation of edikang ikong (vegetable soup)                                                   43

3.3       Experimental Design And Data Analysis                                                           45

3.4       Container Examination                                                                                       46

3.4.1    Visual examination                                                                                             46

3.4.2    Static load burst test (compression test)                                                             48

3.5       Proximate Analysis                                                                                             49

3.5.1.   Moisture determination                                                                                       49

3.5.2    Determination of fat                                                                                           49

3.5.3    Determination of protein                                                                                    50

3.5.4    Determination of crude fibre content                                                                 51

3.5.5    Determination of total ash content                                                                     52

3.5.6    Determination of carbohydrate                                                                            52

3.5.7    Determination of total solids                                                                              52

3.6       Mineral Analysis                                                                                                 52

3.7       Microbial Analyses                                                                                             53

3.7.1    Total viable count                                                                                               53

3.7.2    Total fungal count                                                                                               54

3.8       Shelf Life Studies With Physicochemical Properties                                         54

3.8.1    Thiobarbituric acid (tba) analysis                                                                        54

3.8.2    Free fatty acid (ffa) analysis                                                                               55

3.8.3    Peroxide value                                                                                                     55

3.8.5    Determination of pH                                                                                            56

3.8.6    Total soluble solids determination                                                                        56

3.8.8    Determination of viscosity                                                                                    57

3.8.9    Determination of colour                                                                                         57

3.9       Analysis of Water Soluble Vitamins                                                                     57

 

CHAPTER 4: RESULTS AND DISCUSSION

4.1       Proximate Compositions of The Soup Samples                                                    59

4.1.1    Moisture content of the soup samples                                                                   59

4.1.2    Ash content of the soup samples                                                                          62

4.1.3    Fat content of the soup samples                                                                           63

4.1.4    Crude fibre content of the soup samples                                                              64

4.1.5    Protein content of the soup samples                                                                     65

4.1.6    Carbohydrates content of the soup samples                                                         66

4.2       Changes in Proximate Composition of Individual Soups after 5 Months

of Storage                                                                                                             67

4.2.1    Proximate composition of afere ndek iyak soup after storage                  67

4.3       Proximate Composition of The Afang soup                                                          70

4.4       Proximate Composition Of Edikang Ikong Soup                                                74

4.5       Micronutrient Composition Of The Soups                                                           77

4.5.1    Iron contents of the soups                                                                                    77

4.5.2    Calcium composition of the soups                                                                       80

4.5.3    Thiamine contents of the soups                                                                            82

4.5.4    Ascorbic acid contents of the soups                                                                     83

4.6       Physicochemical Properties of the Soup Samples                                                 84

4.6.1    Free fatty acid composition of the soup samples                                                  84

4.6.2    Viscosity of the soup samples                                                                                85

4.6.3    Thiobarbituric acid value of the soup samples                                                      87

4.6.4    Peroxide value of the soup samples                                                                      88

4.6.5    pH values of the soup samples                                                                              89

4.6.6    Total soluble solids of the soup samples                                                               89

4.6.7    Colour properties of the soup samples                                                                   90

4.7       Microbial Load of Produced Local Soup Samples                                               91

4.7.1:   Effects of storage on the microbial properties of the soup at

ambient temperature                                                                                             91

4.7.2    Effects of refrigeration and freezing storage on total microbial

counts of local soups for months one, two and five                                             93

4.7.3    Effects of refrigeration and freezing storage on total viable

counts of local soups                                                                                             97

4.8       Shelf Life Study of The Soups                                                                             100

4.8.1    Effect of storage on the physicochemical properties of the afang soup               100

4.8.2    Free fatty acids of afang soup                                                                                          100

4.8.3    Thiobarbituric acid value of afang soup                                                               102

4.8.4    pH value of afang soup                                                                                         103

4.8.5    Viscosity value of the afang soup                                                                         104

4.8.6.   Peroxide value of the Afang soup                                                                         105

4.9       Effect of Storage On The Vitamin And Mineral Composition of The

 Afang Soup                                                                                                          105

4.9.1    Vitamin c composition of the afang soup                                                             107

4.9.2    Vitamin b1 composition of the afang soup                                                           107

4.9.3    Calcium composition of the afang soup                                                               108

4.9.4    Iron composition of the afang soup                                                                      108

4.10     Effect of Storage On The Physicochemical Properties Of The

Afere Ndek Iyak Soup                                                                                           109

4.10.1  Free fatty acids of afere ndek iyak soup                                                               109

4.10.2  Thiobarbituric acid value of afere ndek iyak soup                                                112

4.10.3  The pH value of afere ndek iyak soup                                                       113

4.10.4  Viscosity value of the afere ndek iyak soup                                                          114

4.10.5  Peroxide value of the afere ndek iyak soup                                                          114

4.11     Effect Of Storage On The Vitamin And Mineral Composition Of The

Afere Ndek Iyak Soup Soup                                                                                  115

4.11.1  Vitamin C composition of the afere ndek iyak soup                                             115

4.11.2  Vitamin B1 composition of the afere ndek iyak soup                                           118

4.11.3  Calcium composition of the afere ndek iyak soup                                                118

4.11.4  Iron composition of the afere ndek iyak soup                                                       119

4.12     Effect Of Storage On The Physicochemical Properties Of The

Afere Ndek Iyak Soup                                                                                           119

4.12.1  Free fatty acids of afere ndek iyak soup                                                               119

4.12.2  Thiobarbituric acid value of edikang ikong soup                                                  121

4.12.3  The pH value of edikang ikong soup                                                                    122

4.12.4  Viscosity value of the edikang ikong soup                                                           123

4.12.5  Peroxide value of the edikang ikong soup                                                            124

4.13.1  Effect of storage on the vitamin and mineral composition of the

edikang ikong soup                                                                                               124

4.13.2  Vitamin c composition of the edikang ikong soup                                                126

4.13.3  Vitamin b1 composition of the edikang ikong soup                                             126

4.13.4  Calcium composition of the edikang ikong soup                                                  127

4.13.5  Iron composition of the edikang ikong soup                                                         127

4.14.    Response Surface Analysis Of The Factors Affecting The Storage Of The Soups

For Afere Ndek Iyak                                                                                              128

4.15     Thiobarbituric Acid Contents Of The Soups                                                        129

4.16     The Free Fatty Acid Contents Of the Soups                                                       131

4.17     The Peroxide Value of the Soups after Two Months Storage                             134

4.18     The pH Contents the Soups after Two Months Storage                                       137

4.19     The Iron Contents the Soups after Two Months Storage                                     140

4.20: Optimization of the Quality of the Soups Stored For Two Months                        142

 

CHAPTER 5: CONCLUSION AND RECOMMENDATION

5.1   Conclusion                                                                                                                144

5.2   Recommendations                                                                                                    145

References                                                                                                                        147

Appendices                                                                                                                       153

 






LIST OF TABLE


            3.1:      Recipe for Afang Soup (African Salad)                                                   47

3.2:      Recipe for Fisherman Soup                                                                       49

3.3       Ingredients for Edikang ikong (Vegetable soup)                                      51

3.4:      Response surface design coded variables for storage stability

evaluation of packaged indigenous soups                                                54

3.5:      Actual variable settings for response surface design for storage stability evaluation of packaged indigenous soups                                                   55

4.1:      Proximate Composition of Soup Sample                                                  60

4.2:      Proximate compositions of Afere ndek iyak soup and its effect on

storage stability                                                                                         68

4.3:      Proximate compositions of Afang soup and its effect on

storage stability                                                                                         73

4.4:      Proximate compositions of Edikang ikong soup and its effect on

storage stability                                                                                         76

4.5:      Micronutrient Composition of the Soups                                                 80

4.6:      Physicochemical Properties of the Soup Samples                                       85

4.7:      Effect of storage on total viable counts (X 108 CFU/mL) of local

soups stored at ambient temperature                                                          92

4.8:      Effect of Storage on Total fungal Counts (X 108 CFU/mL) of

local soups stored at ambient temperature                                                  93

4.9:      Effects of refrigeration and freezing storage on total viable counts

(X 108Cfu/mL) of packaged local soups                                                    96

4.10:    Effects of refrigeration and freezing storage on total fungal counts

(x 108cfu/mL) of packaged local soups                                                      99

4.11:    Effect of storage on the physicochemical properties of

The Afang soup                                                                                         101

4.12:    Effect of storage on the vitamin and mineral composition of the

Afang soup                                                                                                106

4.13:    Effect of Storage on the physicochemical properties of

Afere ndek iyak                                                                                          111

4.14:    Effect of storage on the vitamin and mineral of the

Afere ndek iyak soup                                                                                 117

4.15:    Effect of storage on the physicochemical properties of the

Edikang ikong soup                                                                                   120

4.16:    Effect of storage on the vitamin and mineral of the

Edikang ikong soup                                                                                   125

4.16.1: Table of Coefficients for terms of response surface models

for quality properties of packaged and stored local soups                       128

4.17:    ANOVA for Linear model for the thiobarbitituric acid (TBA)

concentration in local soups packaged and stored for two month           129

4.18:    Coefficients in Terms of Coded Factors model for the TBA

concentration in local soups packaged and stored for two months          130

4.19:    ANOVA for Linear model for the free fatty acid (FFA) concentration

in local soup packaged and stored for two months                                  132

4.20:    Coefficients in Terms of Coded Factors for linear model for the FFA

 concentration in local sops packaged and stored for five months           133

4.21:    ANOVA for Linear model for the peroxide value (PV) of local

soups packaged and stored for two months                                                    135

4.22:    Coefficients in Terms of Coded Factors for linear model for the

peroxide value of local soups packaged and stored for five months        136

4.23:    ANOVA for two factor interaction model for the pH of local soup

packaged and stored for two months                                                                   138

4.24:    Coefficients in Terms of Coded Factors for two factor interaction

model for the pH of local soups packaged and stored for five months    139

4.25:    ANOVA for linear model for the iron content of local soups packaged and stored for two months                                                                              140

4.26:    Coefficients in Terms of Coded Factors for linear model for the iron

content of local soups packaged and stored for five months                   141

 

4.27:    Constraints for the optimization of the quality of packaged local soups

stored for two month                                                                                142

4.28:    Solutions from the optimization of the quality of packaged local soups

stored for two months                                                                              143

 

 

 

 

 


LIST OF FIGURES

TITLE                                                                                                                             PAGES

Figure  3.1:      Flow chart for the preparation of packaged Afang soup (African salad)

(Faleti, 1999)                                                                                              40

Figure 3.2:       Flow chart for the preparation of packaged Fisherman soup

(Afere ndek iyak) (Ana, 2000)                                                                    42

Figure 3.3:       Flow chart for the preparation of packaged Edikang ikong

(vegetable soup) (Ana, 2000)                                                                     45

Figure 4.1:       Response surface plot for effect of soup type, packaging materials and

storage condition on the TBA content of packaged soups                       131

Figure 4.2:       Response surface plot for effect of soup type, packaging material and

storage condition on the FFA content of packaged local soups               134

Figure 4.3:       Response surface plot for effect of soup type, packaging material and

storage condition on the peroxide value of packaged and stored local

soups                                                                                                         136

Figure 4.4:       Response surface plot for effect of soup type and storage condition on

the pH of packaged and stored local soups                                              139

Figure 4.5:       Response surface plot for effect of soup type, packaging material and

storage condition on the iron content of packaged and stored local soup141

 

 

 



 

 

CHAPTER 1

INTRODUCTION


1.1       BACKGROUND OF THE STUDY

Soups which are basically liquid food composing of various types of lefy vegetables and other constituents that are usually served with swallows play significant part in the food culture of most West African countries. The utilization of these leafy vegetables is a component of Africa’s cultural heritage, as they play major part in the ordinance, traditions and food culture of most African household (Sanusi and Olurin, 2012). Nigeria is richly blessed with varied types of traditional vegetables which are used in various soup preparations and different types of soups are eaten by the different ethnic groups for various reasons. The nutritious compositions of the various varieties of leafy vegetables vary considerably and unlike the starchy roots and tubers, which form the major portion of typical African diets, they are not the considerable sources of carbohydrates. Rather, they contain vitamins, required amino acids, as well as minerals and antioxidants (Mnzava, 1997; Fasuyi, 2006).

Several studies have shown that malnutrition can be tremendously reduced with an increased use of soups and swallows rich in energy, proteins, iron and vitamins, most especially those soups prepared from fresh vegetables from the rural environment (Richard et al., 2007). In order to have a healthy population that can promote development, Atasie et al. (2009) posit that the interrelation between food example soups and swallows, nutrition and health should be reinforced. One way of achieving this is through the exploitation of available local resources such as local indigenous vegetables, since human population in Africa depends largely on a sizeable quantity of edible indigenous vegetables to meet up with scarcity in minerals and vitamins (Achu et al., 2005).

The interconnection between diet, food habits and micronutrient deficiency diseases has necessitated investigations on the nutrient content of traditional soups consumed in many regions of Nigeria (Obiakor–Okeke et al., 2014). Therefore, the quality of a population’s indigenous or traditional soups, being central in their micronutrients supply force, is quintessential in achieving the desired food security and nutritional adequacy (Sanusi and Olurin, 2012).

According to study by Maduabum (2015), sustaining the availability and supply of these staple soups in safe, nutritious, shelf-stable and ready-to-eat forms would be important and helpful. Besides the soup spoilage due to poor packaging and inadequate storage facilities, most methods of preparation and storage of these traditional soups often lead to large loss of micro nutrients, thus creating a deficiency risk of micro nutrient. Efforts have been directed towards the research on the mineral contents of prepared (ready made) Nigerian soups (Akindahunsi and Oboh, 1999; Elemo et al., 2010). However, their shelf stabilities under common storage conditions have not been reported.

A major feature of the Nigerian food economy is the range of vegetables cultivated. Nigeria is a multi-cultural society with different traditional soups which are indigenous to the different ethnic and cultural societies (Okeke et al., 2008). These soups are noted for their unique combination of vegetables, spices and seasonings, although they are mostly not hot and spicy. Normally, well-made Nigerian soups are carefully balanced with proteins (fish, meat, shrimps and periwinkle), vitamins and minerals (vegetables) to bring out all the different nutrients in a dish (Faleti, 1999). Common soups in South-South Nigeria described by Faleti (1999) and others include, Afang, Editan, Melon (Afere ikon), Afia efere (white soup), Afere Ndek Iyak (Fisher man soup), Edikang Ikong (Vegetable soup), Ogbono (draw soup) and Etidot (bitter leaf) soup. Among the popular vegetables cultivated and used mainly in the preparatory process of different types of soups in the South-East are, the fluted pumpkin (Ugu), Bitter leaf (Olugbu), Camwood leaf (Oha/Nturukpa), Green (Inine), Okro (Okwuru), Vegetable jute (Ahihiara), Fever plant (Nchuanwu), African egg plant (Ofe/Anara), Water leaf (Mgbolodi) and the Elephant grass (Achara). The usage of these vegetables in soup preparation varies among communities and regions (Iwuagwu, 1996, Okeke et al., 2008).

Meanwhile, the packaging of nutritious indigenous soups into convenient forms has started receiving some attentions in recent times with some food vendors putting these indigenous soups in plastic plates termed ‘take away’, although it is mostly viewed as simply being marketing strategies by the vendors. Canning or packaging of indigenous South South Nigerian soups in aluminium pouches or polyethylene bags has not been reported.

Although, canned foods constitute a considerable portion of the modern diet. Some other commonly used packaging materials for food and soup products are the rigid metal and glass containers. Pliable packaging is a new innovation, with other technologies found to be better and safer methods of preserving vegetables, meats, poultry and seafood (Fasuyi, 2006). Soups are rich in oils/fats and foods containing fat are known to spoil by fat oxidation. Storage in low temperature conditions (refrigeration and freezing), keeping out of sunlight and proper oxygen barrier properties, has been recommended (Joseph, 2013). The use of mylar bags (pouches) with spouts, for instance, where the container shrinks along with the stored product leaving less room for oxygen has been recommended for non-solid foods such as oils. Hence, application of these recommendations to soups in this study, where their barrier and other properties may be expected to affect the soup’s keeping quality.

The nutritional characteristics of people in developing Nations such as Nigeria seems to be experiencing a substle but gradual change from vegetable dense, high fiber, less calorie, minimal protein diets to low fiber, calorie dense, and high protein diets (Popkin, 2002; Maduabum, 2015). The influence of people’s occupation on their nutritional status and daily diets are implicated in this. Maduabum (2015) observed that it is a common practice for working class, especially workers in big, busy cities, such as Lagos State, Nigeria, to leave their homes early daily in order to be able to arrive at their places of work on time. As a result, most of these workers are forced to either skip meals or eat what is available in and around their workplaces. Findings on workers’ eating practices showed that a great percentage of working class either skip breakfast and lunch, eat their breakfast at work, carry breakfast in a food flask from home to work, buy food from canteens within their workplace, buy food from fast food outlets, buy snacks to eat as lunch, eat in between meals, and buy breakfast or lunch from food vendors, among others (Eze et al., 2017).

When many variables affect an outcome to be studied, response surface methodology comes handy. RSM is an effective statistical technique for optimizing experimental protocols. RSM evaluates the outcome of multiple factors and their interactions on one or multiple response variables (Azmir et al., 2014). The optimization of those parameters influencing the quality of a product could be achieved with response surface methodology (RSM). An advantage of Response Surface Methodoloy is that, it decreases the number of experiments and provides a mathematical model (Dongliang et al., 2018).


1.2       STATEMENT OF PROBLEM

As the food industry continues to grow, food production also increases and this increase must be sustainably managed. One such way of sustainably managing this increase is by preservation. Several researches have been performed on the mineral, vitamins, physicochemical and sensory properties of the various local soups in Nigeria, but none of these studies examined the delivery of Afang (Gnetum afrikanum) soup, Edikang ikong (Vegetable soup), Afere ndek iyak (Fisherman soup) and Afia efere (White Soup) in shelf stable, convenient forms, suitable for easy distribution to other places (Brat and Hammond, 2010). Furthermore, cravings for convenient foods have continued to soar, becoming a part of the modern society. Also, experience and interaction with Nigerians living in the country and in diaspora has revealed that they crave for fresh nutritious indigenous soups, notwithstanding the cost.

Interest in these soups has continued to increase due to their rich nutrient contents. While Afere Ndek Iyak soup is known especially for the heavy protein and unsaturated fat contents due to its high seafood-dense nature, edikang ikong and afang soups are noted for their wealth of micronutrients (Obiakor-Okeke et al., 2008; Kayode et al., 2010) and fibre (Ani et al., 2011). For instance, a study on the vitamins and minerals contents of selected soups consumed in South-South Nigeria conducted by Kayode et al. (2010) showed that Edikang-Ikong and Afang soups had the highest concentrations of Iron, calcium, phosphorus, etc. These are all in combination with the rich sensory appeal of these intercontinental delicacies (Faleti, 1999, Ana, 2000).

Soups, like other food products, are bound to deteriorate in quality with time after production, if not properly preserved. Even soups that are refrigerated are bound to lose important nutrients and their nutritional values as a result of thawing and the reheating process required before consumption. Most soups spoil readily as a result of their high water activities, fat or protein contents. Due to its rich protein and fat contents, Afere Ndek Iyak (fisherman) soup in particular, spoils very easily. Hence, making the soups readily available in a safe, shelf-stable and convenient form remains a problem.

Although canning, besides the ease of transportation (Richard et al., 2007), sustains shelf stability of foods for one to five years, even with the possibility of being much longer under certain circumstances (Field et al., 2002) without requiring refrigeration (Richard et al., 2007), the technological requirements are deep and high in cost.  Furthermore, reports have shown that although canned foods may not be found to be containing trace of microbial growth after long stay, sensory properties such as appearance and smell, and vitamin content can deteriorate to unacceptable levels (Mnzava, 1997, Fasuyi, 2006). This is in combination with the risks of destroying nutrients during the sterilization, and the toxicological effects or loss of organoleptic properties due to chemical contamination of the packaged food by the lacquering in the can (Abdel-Rahman, 2015). Presently, the quest to consume canned (especially tin cans) foods is decreasing (Nair and Girija 1994; Dileep and Sudhakara, 2007) as a result of the high cost of tin for making cans acceptable to the market (Srivasta et al., 1993). Reports of tin poisoning from canned food have also raised safety concerns over canned food (Abdel-Rahman, 2015). Hence, the problem of finding cheap, safe and simple alternative to canning lingers.

There is therefore the need to produce, package and preserve indigenous Nigerian soups in aluminium pouch, polyethylene bag and plastics which may be considered an alternative to canning and these packaging materials are cheap, safe and readily available. This will satisfy the yearnings of Nigerians in the country and in diaspora that craves for these covinient packed indigenous soups, and also fills the knowledge gap on the packaging and preservation of South South (Indigenous) Nigerian soups. It is in view of the need to fill this knowledge gap and ascertain which packaging materials or storage condition is the best to preserve these soups for extended period of time that this research was conducted.


1.3       JUSTIFICATION OF THE STUDY

With the increasing population of Nigerians at home and in diaspora and the global population in general, the demands for indigenous/ ethnic delicacies will continue to soar. Besides the intended academic break through, packaging these soups in ready-to-eat forms would also come as a great relief since the preparation of most of these soups takes quite a process.

Besides raising the nutritional standard of the people, the increased food production, therefore, suggests it is essential to sustain increased production by exploring and expanding the horizon of packaging ready to eat vegetable soups in containers easy to come by. The success in shelf stability of indigenous soups packaged in these simple materials would, apart from creating convenience, also allow the consuming public to have their varied, nutritious, indigenous diets during all seasons, with improved ease of  distribution to all manner of consumers in many local, regional and possibly, geographical locations. Packaging of various sauces, stew and broths have been carriedout by several workers, report on traditional and heritage Nigerian soups are deficient, hence, the attempt to standardize their packaging in ready-to-eat styles. Many researchers have researched on the effects of packaging material and storage coditions on the post harvest shelf life and quality of some individual soup ingredients and vegetables. These studies were not extended to storage conditions with appropriate packaging techniques for shelf stability of their finished products in soups. Therefore, the present study sees necessity to assess the effects of packaging materials (Polyethylene bags, aluminium pouches and plastic jars) under ambient and regulated low temperature storage on shelf stability of the soups.

In real situation, many factors are known to affect the quality of a product. It is not economically favorable to consider all possible levels of the factors at a time. Also, it is feasible to predict and optimize processes and responses using appropriate design models. The versatility of response surface models in achieving this is widely reported. The use of response surface in this study therefore becomes justifiable. With this methodology, the modeling of the quality parameters of the soup, as well as optimization of the packaging and storage conditions could be achieved. 


1.4       AIM AND OBJECTIVES OF THE STUDY

The principal aim of the research was to assess the effects of packaging materials as well as storage conditions on stability of selected indigenous soups of South- South Nigeria during storage.

Specific Objectives Include:

1)      Prepare Afang, Edikang Ikong and Afere Ndek Iyak soups

2)      Package each of the soups in polyethylene, aluninium pouches and plastic jars and store at ambient (25oC), refrigeration (4 oC) and freezing (-18 oC) conditions for 5 months

3)      Determine proximate and selected micronutrient constituents of the soup periodically

4)      Determine oxidative parameters of the soup periodically

5)      Determine microbiological quality of the soups periodically

 

 

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