EFFECT OF HYPTIS SUAVEOLENS LEAVES EXTRACT ON ADULT MOSQUITO POPULATION

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Product Code: 00008390

No of Pages: 56

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ABSTRACT

Mosquitoes are major vectors that transmit disease like Malaria, Dengue fever, Leishmaniasis, Filariasis and other diseases. Disease caused by mosquitoes  have been of economic importance. This research evaluate the efficacy of Hyptis suaveolens  leaf extract on adult mosquito population, the plant were collected from it natural environment within Dutse metropolis at warwade dam Dutse Jigawa state.The plant leaves were air dried and extract was obtained using sohxelet extractor, four group of 20 mosquitoes each were sprayed with the dissolved extract at concentractions of 100mg/ml, 300mg/ml and 500mg/ml. The result shows that high concentration give the highest mortality, this signify that the greater the concentration, the greater the effect of the leaf extract on the mosquito population. The phytochemicals found to be responsible for these activities are Saponins, Phlabotonins, Tannins and Phenones, The study conclude that Hyptis suaveolens have effect on Mosquito population.




 Table of Contents

DECLARATION…………………………….………………………………………………………i

CERTIFICATION…………………………………………………………………………………..ii

DEDICATION……………………………………………………………………………………...iii

ACKNOWLEDGEMENT……………………………………………………………………….…iv

ABSTRACT…………………………………………………………………………………………v

TABLE OF CONTENTS…………………………………………………………………………...vi


CHAPTER ONE. 1

INTRODUCTION.. 1

1.1 Background. 1

1.2. Statement of Research Problem.. 4

1.3 Justification. 4

1.4  Aim and Objectives. 4

1.4.1 Aim.. 4

1.4.2 Objectives. 4

1.5 Research Hypothesis. 5


CHAPTER TWO.. 6

2.0 LITERATURE REVIEW... 6

2.1 Biology of Mosquitoes. 6

2.2 Distinguishing Features of Mosquitoes. 7

2.3 Life Cycle. 8

2.4 Biting Behaviour. 11

2.5 Distinguishing Features of Vector Mosquitoes. 11

2.6 Anopheles Mosquitoes. 13

2.7 Culex Mosquitoes. 16

2.8 Aedes Mosquitos. 17

2.10 Modes of Action against Specific Life Stages. 20

2.11. Pesticides. 20

2.13 Insecticide. 22

2.14 Biopesticides. 23

2.15 Types of biopesticide. 24

2.16 Description of Hyptis suaveolens. 28

2.17 Phytochemicals. 29


CHAPTER THREE. 30

3.0 MATERIALS AND METHOD.. 30

3.1 Materials. 31

3.2 Study Area. 31

3.3 Plant Material 31

3.4 Preparation of Crude Extract. 31

3.5 Source of Adult Mosquitoes. 31

3.6 Preparation of Stock Solution. 32

3.7 Experimental Design. 32

3.8 Phytochemical Screening. 32


CHAPTER FOUR. 33

4.0 RESULTS. 33

4.1 Phytochemical Screening of the leaf Extracts. 34

4.2  Mortality Rate of Mosquitoes exposed to H. suaveolens leaf extract. 34


CHAPTER FIVE.. 38

5.0 DISCUSSION, CONCLUSIONS AND RECOMMENDATIONS. 38

5.1 Discussion. 38

5.2 Conclusion. 39

5.3 Recommendation. 40

REFERENCES. 40







CHAPTER ONE

INTRODUCTION


1.1 Background

Mosquitoes play a unique function as vectors for many diseases; these diseases often have a wide distribution and a high number of cases. Because of its role as a vector, mosquito-borne diseases kill more people worldwide each year than any other single factor (Brown,1986). To prevent proliferation of mosquito borne diseases and to improve quality of environment and public health, mosquito control is essential. The major tool in mosquito control operation is the application of synthetic insecticides such as organochlorine and organophosphate compounds. But this has not been very successful due to human, technical, operational, ecological, and economic factors. In recent years, use of many of the former synthetic insecticides in mosquito control programme has been limited. It is due to lack of novel insecticides, high cost of synthetic insecticides, concern for environmental sustainability, harmful effect on human health, and other non-target populations, their non-biodegradable nature, higher rate of biological magnification through ecosystem, and increasing insecticide resistance on a global scale (Brown,1986, and Russell et al.,2009).Thus, the Environmental Protection Act in 1969 has framed a number of rules and regulations to check the application of chemical control agents in nature. It has prompted researchers to look for alternative approaches ranging from provision of or promoting the adoption of effective and transparent mosquito management strategies that focus on public education, monitoring and surveillance source reduction and environment friendly least-toxic larval control. These factors have resulted in an urge to look for environment friendly, cost-effective, biodegradable and target specific insecticides against mosquito species. Considering these, the application of eco-friendly alternatives such as biological control of vectors has become the central focus of the control programme in lieu of the chemical insecticides.

One of the most effective alternative approaches under the biological control programme is to explore the floral biodiversity and enter the field of using safer insecticides of botanical origin as a simple and sustainable method of mosquito control. Further, unlike conventional insecticides which are based on a single active ingredient, plant derived insecticides comprise botanical blends of chemical compounds which act concertedly on both behavioural and physiological processes. Thus there is very little chance of pests developing resistance to such substances. Identifying bio-insecticides that are efficient, as well as being suitable and adaptive to ecological conditions, is imperative for continued effective vector control management. Botanicals have widespread insecticidal properties and will obviously work as a new weapon in the arsenal of synthetic insecticides and in future may act as suitable alternative product to fight against mosquito borne diseases.

Roark (Roark, 1947) described approximately 1,200 plant species having potential insecticidal value, while (Sukumar et al.,1991) listed and discussed 344 plant species that only exhibited mosquitocidal activity. Shallan et al.,2005) reviewed the current state of knowledge on larvicidal plant species, extraction processes, growth and reproduction inhibiting phytochemicals, botanical ovicides, synergistic, additive and antagonistic joint action effects of mixtures, residual capacity, effects on non-target organisms, resistance and screening methodologies, and discussed some promising advances made in phytochemical research.

The mosquitocidal activities of various herbal products from edible crops, ornamental plants, trees, shrubs, herbs, grasses and marine plants according to the exaction procedure developed in eleven different solvent systems and the nature of mosquitocidal activities against different life stages of different vector species as a ready reference for further studies.

The use of plant extracts to control parasitic infections has been a strong interest of researchers around the globe, carrying the potential for development of alternative control strategies. In selecting plants to test as agents against mosquitoes, it may be useful to include endogenous knowledge about plant resources. Including plants which have been used as traditional medicines allows researchers to incorporate the observations of a wider variety of experiences. While plant selection should draw from a broader inventory, standard testing regimens for potential insecticides should be utilized by researchers in order to produce results that facilitate comparative analyses (CTD/WHO PES/IC, 1996; WHO CDS/ WHO PES, 2005). It is important that these efforts include careful analysis of the solvents used in the extraction or re-suspension process to ensure that adequate controls exist for comparison between the active compounds and the solvent (Zahir et al., 2009). Utilizing endogenous knowledge concerning plants with traditional medicinal value has proven fruitful in identifying potential sources of phyto-extracts with insecticidal activity (Rahuman, et al., 2009), There have been many attempts to assay the activity of particular plant extracts against vectors of human disease, in particular through the utilization of plants for which such knowledge exists (Mathew, et al., 2009). Recent studies using these approaches have yielded some promising plants with larvicidal activity. Essential oils that have indicated adult repellence have also been shown to function as larvicides, indicating a cross-functional potential for utilizing these natural products (Zhu, et al., 2008). Therefore this project will provide more information to scientific and local community on the effect of Hyptis suaveolens on mosquito .The plant is reported to possess repellent activity against insect pests of stored grains and also use as treatment of many disease.


1.2. Statement of Research Problem

Mosquitoes serve as vector of tropical and subtropical parasites that cause diseases like Dengue fever, Dengue haemorrhagic fever, Malaria, Japanese encepahalitis and Filariasis that afflict millions of people worldwide (Service, 1983; Gubler, 1998; WHO 1992). There have been various researches on the use of plant exract against mosquito but on the use of Hyptis  suaveolens against adult mosquitoes there is no/little documented research work.

1.3 Justification

Mosquitoes are the oldest human enemy and represent a significant threat to human health because of their ability to vector pathogens that cause diseases like Dengue fever, Dengue haemorrhagic fever, Malaria, Japanese encepahalitis and Filariasis that afflict millions of people worldwide (Service, 1983; Gubler, 1998; WHO 1992). The continuous use of synthetic insecticides, its toxicity problem together with the growing incidence of insect resistance has called the need for novel insecticide. Plant extracts may be alternative sources that constitute a rich source of bioactive compounds that are biodegradable and environmentally friendly.

1.4  Aim and Objectives

1.4.1 Aim

This research is aimed at evaluating the effect of Hyptis suaveolens leaf extracts on adult mosquitoes.

1.4.2 Objectives

1. To determine the phytochemical constituents of the  extract.

2. To determine the effect of hyptis suaveolens leave extract on adult  mosquitoes.


1.5 Research Hypothesis

  There are no active phytochemicals in the  Hyptis suaveolens extract.

 There is no significant effect of Hyptis suaveolens leave extract on adult mosquitoes.  



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