PRODUCTION OF MOSQUITOES REPELLANTS INSECTICIDES (MOSQUITO COIL) USING ORANGE PEELS (CESTRUM)

DDT (Dichloro-Diphenyl-Trichloroethane)[1]

DDT was first prepared by O. Zeidler, a German Ph.D. student in 1874. Its insecticidal properties were however discovered by a Swiss chemist Paul Muller in 1939. DDT kills lice and mosquitoes which carries germs of typhus and malaria fever respectively. DDT was the first chemical to have the property of killing insects only by means of contact. DDT is still the backbone of many insects control programs and is widely used as a household insecticide against leaf hoppers and potatoes and in cotton insect control. DDT is a remarkable powerful and persistent insecticide that is soluble in kerosene oil, petrol and ethyl alcohol but insoluble in water. DDT is manufactured by exothermic condensation of chlorobenzene with chloral at about 30⁰C in the presence of oleum or 99% sulphuric acid.

DDT (Dichloro Diphenyl Tdrichloroethane) Fig II [1]

In the 1940s, the properties of the new insecticide DDT seemed close to miraculous. In tropic, DDT save millions of lives by killing the mosquitoes that spread malaria, increase crop yields resulting from DDT’s destruction of insects pests saved million or more from starvation

DDT and other substances that undergo biological manifestation have two properties that makes them dangerous;

§     They do not readily break down into harmless substances

§     They are fat soluble but not water soluble, therefore, they accumulate in the bodies of animals particularly in the fat, rather than being broken down and excreted in the watery urine. Because the transfer of energy from lower to higher tropic level is extremely inefficient, herbivores must eat large quantities of plant materials (which may have been spread with DDT), carnivores must eat many herbivores and so on.

In1951, the first DDT resistant strains of mosquitoes were reported from Greece, Panama and U. S. A. In many parts of the world, spraying no longer prevents transmission of malaria. In addition, the passage of persistent insecticides such as DDT though the food chain become an increasing problem to birds and mammals at the top of chain[4].

1.3.4           BIOLOGICAL INSECTICIDES

Recent efforts to reduce bread spectrum toxins added to environment have brought biological insecticides back to vogue. An example is the development and increase in use of Bacillus thuringieness, a bacterial disease of Lepidopterans and some other insects. It is used as a lavicide against a wide variety of caterpillars. Because it has little effect on other organism. It is considered more environmentally friendly than synthetic insecticides. The toxin forms. Baccillus Thuringiensis. Baccillus Thuringiensis. Toxin has been incorporated directly into plants through the use of genetic engineering[7].

1.3.5 TOXIC EFFECT OF REPELLENT

Some insecticide kill or harm other creatures in addition to those they are intended to kill. For example, birds may be poisoned when they eat food that was freshly sprayed with insecticides or when they mistakes insecticides granules on the ground for food and eat them.

Sprayed insecticides may drift the area to which it is applied into wildlife areas, especially when sprayed aerially[4].

DDT can be a threat to health or the environment when used under wrong conditions. It causes progressively higher concentration in the body of the animal farther up the food chain.

Biological parameters were used to evaluate the toxic effect of different brands of mosquito coil smoke in experimental rats. The smoke from the coil produced significant increase (P<0.05) in the level of total protein, total albumen, bilirubin and blood/urea nitrogen when animals were exposed to smoke for 14 days[7].

Similarly, the smoke from the coils also cause an elevation in the activities of aspirate amino transference and alanine amino transference. Although the smoke from the coil did not produce lesions in hearts, lungs and liver examined, the increase in liver enzyme activities could be due to early liver damage[8].

Epidemiological studies have shown that long-term exposure to mosquito coil smoke can induce asthma and persistent wheeze in children. These studies also shows that one burning mosquito coil produces the same amount of particulate mass (diameter up to 2.5nm) as 75-137 burning cigarette would, and the coil can be as high as that released from 51 burning cigarettes[8].

1.4    MOSQUITO COIL

Mosquito coil is a mosquito repelling incense usually shaped into a spiral and typically made from a dried paste of pyrethrum powder. Mosquito coils are widely used as mosquito repellants. The major active ingredients of mosquito coilsare purethrins accounting for about 0.3 – 0.4% of the coil mass. When a mosquito coil is burnt, the insecticides evaporates (pyrethrin, PAH, aldehyde, etc) with smoke, which prevent the mosquito from entering the room and harm those already in the room. The remaining components of mosquito coils include filters, binders, dyes and other additives capable of burning well without flame. He components generates large amunt of submicrometer particles and gaseous pollutants such as acenephthene, paranthrene, etc.

Mosquito coils are often used overnight in sleeping quarters where continous exposures may occur. Chronic exposure to coil smokes occur during rainy periods because mosquitoes are found to be more active in the environment due to collection of water and increase in green plants[9].

1.4.1 HOW MOSQUITO COILS WORK

Mosquito coils are burned on specially designed stands placed inside more attractive looking mosquito coils holders which contain holes to let the smoke out. The holders can usually be hung up or laid flat. Each mosquito coil slowly burns for around 8 hours, during which time repelling active ingredients such as pyrethoids or pyrethrum disperse with the smoke. The combination of ingredients in the smoke coil either prevents mosquitoes from entering the surrounding area, encourages them to leave the area if they have not already done so, or knocks the insect down and kills the[10].

1.4.2 HOW TO USE MOSQUITO COILS

Mosquito coils are particularly useful for protection against mosquitoes when sitting outside at night. They can be placed on a table so people can congregate around them. It helps to stay in the vicinity of the smoke given off, but this may be difficult if the smoke is carried away by the wind. A good tip is to place a mosquito coil down on the ground by people’s ankle and feet, as this is an area mosquitoes seem to bite frequently.

Again, the less wind there is, the more likely the mosquito coil smoke will have a chance to be effective. Mosquito coils are generally meant for use outdoors, but if they have to be used indoors, it is wise to make sure rooms are well ventilated. Packs of multiple mosquito coils are available with a coil stand included[10].

1.4.3 ADVANTAGES OF USING A MOSQUITO COIL

Mosquito coils nowadays burn without flame for up to eight hours of continuous repelling action. In quantitative tests, they provide about 80% protection. Mosquito coils are also cheep and need no special equipment to use it in other than just lighting it up. They are portable and fit into normal household practices of lighting candles or incense[11].

1.4.4 DISADVANTAGES OF USING MOSQUITO COILS

There are several hazards that we know to be caused by mosquito coils. In 1999, sparks from mosquito coils ignited a fire that swept through a three-story dormitory building at a summer camp, 23 persons including 19 children died in the blaze in South Korea[12].

The long-term exposure calls for concerns on the potential toxicological effects of smoke on humans[8].

1.4.5    ACTIVE INGREDIENTS IN MOSQUITO COIL

The active ingredients found in mosquito coils can be some of the following;

     ·        PYRETHRUM (Natural, powdered material from a kind of chrysanthernum plant, performing moderated)

     ·        PYRETHRINS (Extract of insecticidal chemicals in pyrethrum)

     ·        ALLETHRIN Some times d-trans-alletrin)

      (The first synthetic pyrethrin)

     ·        ESOBIOTHRIN (a form of allethrin)

     ·        DIBUTYL HYDROXYL TOLUENE (BHT) (an optional additive to prevent pyrethroid from oxidizing during burning).

     ·        PIPERONYL BUTOXIDE (PBO) (an optional additive to improve the effectiveness of pyrethroid)

     ·        N-(2-ethylexyl)-bicyclo-(2,2,1)hept-5-ene-2,3-dicarboximide (MGK 264) (an optional additive, to improve effectiveness of a pyrethroid)[13].

1.5       CITRUS SINENSIS/ORANGE PLANT

The orange plant is a hybrid of ancient cultivated origin, possibly between Pomelo (citrus maxima) and tangerine (citrus reticulate). It is small flavouring tree growing to about 10m tall with evergreen leaves, which are arranged alternatively, of ovate shape with crenulate margins and 4-10cm long. The orange fruit is a hesperidium, a type of berry[14].

Orange originated in southeast Asia. The fruit of Citrus sinensis is called sweet orange to distinguish it from citrus aurantium, the bitter orange. The name is thought to ultimately derived from the sanskirt, for the orange tree, with its final form developing languages. In a number of languages.

In a number of language,it is known as a “Chinese apple” (e.g Dutch Sinaasappel, “China’s apple”.)[15].

Orange can be found in almost all parts of Nigeria.

SPECIES OF CESTRUM                                                                The orange peels (cestrum) is gotten from sweet orange (ctrus sinensis)

1.5.1 IDENTIFICATION

Botanical name          Citrus Sinensis

English name             Sweet Orange

Common name`                   Oroma (Igbo)

Family                           Rutaceae

Genus                          Citrus

Order                            Sapindales

1.5.2 USES OF CITRUS SINENSIS

1.  1.   In the home,oranges are commonly peeled segmented and utilized in fruit cups, salads, gelatins and numerous other deserts and as garnishes on cake, meats and poultry dishes.

     2.   They are squeezed daily in the kitchen for juice.

     3.   In South America, a dozen whole, peeled oranges are boiled in 3 pints (1.41 litres) of slightly sweetened water for 20min and then strained and the liquid is poured over small squares of toast and slices of lemon and served as soup.

    4.   Whole oranges are sliced, dried and pulverized and the powder is added to baked goods as flavoring.

      5.   Dried orange peels can be used as home made bath oils.

    6.   Limonene carbon-base compounds that makes up around 95% of the oil found in orange peels is often used to give household cleaners a citrus smell.

    7.   range peels can be applied on the skin, in the night to repel mosquitoes.

     8.   Orange peels can be used as scent.

1.5.3 FOOD VALUE.

The Chemistry of the Orange is affected by many factors. On the average, “Valencia”, Washington Navel”, and other commercial oranges have been found to poses the value shown in the page.

FOOD VALUE PER 100G OF EDIBLE PORTION

1.5.4 ECONOMIC IMPORTANCE/USES

PULP:        citrus (3/4 being a by-product of orange juice extraction) is highly valued as pelleted stocked feed with a protein content marketed as cat litter. It is a source of edible yeast non-potable alcohol, ascorbic, and hesperidin.

PEEL:        In addition to its food uses, orange peel oil is a prized scent in perfume and soaps because of its 90-95% limestone content, it has a lethal effect on mosquitoes, houseflies, fleas and fireants. Its potential as an insecticide is under investigation. Its being used in engine cleaners and in waterless hand-cleaners in heavy mechanical repairs shops.

Terpenes extract from the outer layer of the peels are important in resins and in formulating paints for ships.

SEED:       Oil derived from orange and other citrus seeds is employed as a cooking oil and in soap and plastics. The high- protein seed residue is suitable for human food and an ingredient in cattle feed, and the hills enter into fertilizer mixtures.

FLOWER AND FOLIAGE:  The essential oils distilled from orange flowers and foliage are important in perfume manufacturing. Some petitgrain oil is distilated from the leaves, flowers, twigs and small whole unripe fruits.

WOODS:   The wood is yellowish, it has been valued for furniture, cabinet work, turnery and engraver’s blocks.

Branches are fashioned into walking-sticks.

1.5.5 MEDICAL USES/IMPORTANCE

      ·        Orange are eaten to allay fever and catarrh

      ·        The roasted pulp is prepared as a poultice for skin disease.

      ·        The fresh peel is rubbed on acne

      ·        In the mid-1950s, the health benefit of eating peeled, whole oranges was much publicized because of its protopectin, bioflavonoids and inositol (related to vitamin B).

      ·        Rutin and other bioflavonoids were for a while much advocated for treating capillary fragility, hemorrhages and other physiological problems.

      ·        An infusion of the immature fruit is taken to relieve stomach and intestinal complaints.

       ·        The inner bark, macerated and infused in wine, is taken as a tonic and carminative.

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