ABSTRACT
Elemental analysis
is the qualitative detection and quantitative determination of chemical
elements(atoms,ions)in a sample(Fritz Pregl 1923). To detect an
element, one should fix an appearance of an analytical
signal. The formation of precipitate or characteristic crystals, colour change,
an isolation of gaseous products, an appearance of a definite lines in
spectrum, luminescence, etc. To determine elements quantity, it is necessary to
measure a value of an analytical signal; a precipitate mass, intensity of a
current, solution absorption, spectrum line, luminescence or radioactivity, a
reaction rate and so on.
This study was undertaken to analse the elements present in
grounded Pterocarpus mildbreadii (Oha seed) using Atomic Absorption
Spectrometer(AAS MODEL-AA320N).The seed was found to contain these essential
macro minerals/elements sodium(Na),Potassium(K),calcium(Ca),magnesium(Mg),and
the trace elements iron(Fe),copper(Cu),zinc(Zn), and selenium(Se)(Duffus,2002).
The study established that Pterocarpus mildbreadii(oha seed)
does not contain manganese and has high content of potassium which is necessary
for good health.
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TABLE
OF CONTENTS
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Title page
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Approval Page
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Dedication
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Acknowledgement
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Table of Contents
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Abstract
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CHAPTER ONE:
INTRODUCTION, AIMS & OBJECTIVES
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1.1 Introduction
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1.2
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Aim
of study
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1.3
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Research
objective
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CHAPTER
TWO:LITERATURE REVIEW
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2.1
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Elemental
analysis
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2.2
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Trace
elements as catalysts
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2.3
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Biological
functions of metals,sources and deficiency
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2.4
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Pterocarpus
mildbreadii:A case study for elemental analysis
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2.4.1 Systematic
position of Pterocarpus mildbreadii
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2.4.2 General characteristics
of Leguminosae
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2.4.3
Geographical distribution of
Pterocarpus mildbreadii
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2.4.4 Properties
of Pterocarpus mildbreadii
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2.4.5 Botany
of Pterocarpus mildbreadii
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2.4.6 Ecology of
Pterocarpus mildbreadii
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2.4.7 Management
and propagation of Pterocarpus
mildbreadii
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2.4.8 Prospects
of Pterocarpus mildbreadii
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2.4.9 Uses
of Pterocarpus mildbreadii
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CHAPTER
THREE:MATERIALS AND METHODS
3.1
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Materials
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3.1.1
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Equipment/apparatus
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3.1.2
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Chemicals/reagents
used
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3.2
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Methodology
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3.2.2
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Collection
and preparation of plant material
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3.2.3
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Extraction
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3.2.4
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Preparation
of reagent for AAS
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3.3
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Elemental
analysis
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3.4
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Atomic
Absorption Spectrometry
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CHAPTER FOUR:RESULTS
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4.0
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Elemental
content of Pterocarpus mildbreadii
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CHAPTER FIVE:
DISCUSSION
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CONCLUSION
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REFERENCES
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CHAPTER ONE
1.1 INTRODUCTION,
AIMS AND OBJECTIVES
A seed or mature ovule is a
miniature plant with a protective cover in a suspended state of development.
Most seeds contain a built-in food supply called endosperm, orchid is an
exception. The endosperm can be made up of proteins, carbohydrates and fats.
Seed can also be defined as a small embryonic plant enclosed
in a covering called the seed coat, usually with some stored food. It is the
product of the ripened ovule of gymnosperm and angiosperm plants which occurs
after fertilization and some growth within the mother plant(Wikipedia).Seed
protects a plant embryo so that it can grow into a new plant. Many seeds are
edible, such as sunflower seeds, tomato seeds, corn and peas.
Seeds contain three distinct structures. The inside of a
seed contains an embryo, which is a baby plant with a shoot and a tiny root.
The two halves of a seed are stored food that provides the nourishment
necessary for seeds to germinate, or begin growing. Surrounding the seed is a
hard, tough seed coat, which protects the seed during dormancy(Anville 2007).
Most seeds contain a built-in
food supply called endosperm. The endosperm can be made up of proteins,
carbohydrates and fats. Seeds also contain anti-nutrients in
their seed coat. These anti-nutrients includes phytin,
lectin, trypsin inhibitor activity, tannin and cyanide. In addition, they also
contain minerals such as sodium,
potassium,calcium,magnesium,phosphorus,zinc,manganese,iron,selenium and
copper.(Balogun 2000)
Oilseeds are energy dense
foods; for example, sesame seeds provide 600kcal or 2470kj/1000g.Although
oilseeds contain protein(|14-32g/100g)and carbohydrate(ranging from less than
1g/100g to more than 34g/100g),most of the food energy they provide is as fat(which
provides 9kcal or 37kj/g).Oilseeds vary widely in their fatty acid composition
but tend to be rich in MUFA(e.g peanut)r PUFA(e.g sunflower seeds).Some seed
oils contain significant amounts of EFA, ALNA, an n-3 fatty acid, and linoleic
acid(LA),an n-6 fatty acid. from these two fatty acids, the body can make all
the fatty acids it needs. From LA, arachidonic acid can be produced, and from
ALNA the long chain n-3 eicosapentaenoic acid(EPA)and docosahexanoic
acid(DHA)can be made.(BNF 1999).
Generally, whole oilseeds are a
source of fibre, phosphorus, iron and magnesium; many oilseeds are a source of
vitamin E(an antioxidant),niacin and folate. Whole oilseeds also contain
phytoestrogens, a group of substances including lignans and isoflavones.
Phytoestrogens have a structure similar to the oestrogen hormone oestradiol and
can bind to oestrogen receptors.Phytoestrogens may provide a protective effect
against coronary heart disease as they have been shown to have a
lowering effect on blood cholesterol.Additionally,some
phytoestrogens may have antioxidant properties(Goldberg 2003).
In Britain, oilseeds are
usually consumed, following processing,as oils and margarines. The fatty acid
composition of oils produced from oilseeds varies widely. Vegetable oils do not
contain the same levels of macronutrients, vitamins and minerals as whole
oilseeds. In fact, apart from fat itself, vitamin E is the only nutrient
present in appreciable amounts. Vegetable oils do, however, contain a range of
phytochemicals, e.g they are the main source of natural plant sterols in the
diet. Plant sterols have a structure similar to cholesterol and hence reduce
cholesterol absorption, therefore reducing the circulating levels of total and
low density lipoprotein(LDL)cholesterol. Plant sterols can be present as free
or esterified forms and the proportions vary, e.g free sterols dominate in
soybean,olive and sunflower oil,while in rapeseed and corn oil, free sterols
account for only 30% of the plant sterols.Refining vegetable oils decreases the
content of sterols(from 10-70% depending on the oil and processing conditions
used),thus decreasing their potential to lower serum cholesterol(Goldberg
2003).
1.2
AIM OF STUDY
To
determine the elements
present in grinded oha
seed(Pterocarpus
mildbreadii)
1.3 RESEARCH
OBJECTIVE;
This study was primarily designed to use grinded oha seed(Pterocarpus
mildbreadii)
extract for elemental analysis.
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