ABSTRACT
This work evaluated the phytonutrient, antioxidants activity and nutritive qualities of some selected tomato paste products sold in Umuahia metropolis. The phytonutrients, chemical, minerals, and antioxidant activity of tin tomato paste were determined by standards methods. The results showed that lycopene(4.20mg/100g), Vitamin C (12.76mg/100g) and Vitamin A (1.43mg/100g) were significantly (p<0.05) higher in Star tomato paste. Beta carotene (3.02mg/100g) and Vitamin E (11.14mg/100g) were highest (p<0.05) in Clappa paste, while phenol was highest (p<0.05) in Super delicieux paste. Sonia paste showed highest values for total sugar (31.6%) and PH (4.43), while Super delicieux paste had highest Total soluble solid (21.63%) and Clappa paste showed highest reducing sugar (16.16%). On the minerals, Sonia was highest (p<0.05) in calcium (2.36mg/100g) and Fe (1.89mg/100g). Star paste showed highest values of magnesium (13.61mg/100g) and potassium (30.44mg/100g), while in Super delicieux Na (15.37mg/100g) was highest. Strongest antioxidant activity was observed in Sonia paste for DPPH (62.07%), ABTS (41.00%) and FRAP (47.88%) respectively. The result reveled that Sonia tomato paste was the best in most of the measured variables than other paste. From the phytonutrients and antioxidant activity results. These tomato pastes can boost physiological health against degenerative disease.
TABLE OF CONTENTS
Title page
Declaration i
Certification ii
Dedication iii
Acknowledgement iv
Table of contents v
List of Tables viii
List of Figures ix
List of Plates x
Abstract xi
CHAPTER 1: INTRODUCTION
1.1 Background information 1
1.2 Statement of the problem 2
1.3 Objectives of the Study 3
1.4 Significance of the study 3
CHAPTER 2: OVERVIEW OF TOMATO PASTE
2.1 Overview of Tomato Paste 4
2.2 Benefits of Tomato 6
2.3 Free Radicals in Tomatoes 7
2.4 Benefits of antioxidants 8
2.4.1 Antioxidants reduce the risk of cancer 8
2.4.2 Antioxidants protect the heart 9
2.5 Phytonutrients in tomato 9
2.6 Flavonoids 9
2.6.1 Distribution 10
2.6.2 Health Benefits of Flavonoids 10
2.7 Lycopene 11
2.7.1 Distribution 11
2.7.2 Health Benefits of Lycopene 12
2.7.2.1 Anti-cancer 12
2.7.2.2 Antibacterial and antifungal 12
2.7.2.3 Diabetes 12
2.7.2.4 Antioxidant 13
2.7.2.5 Arteriosclerosis 13
2.7.2.6 Antitoxic 13
2.8 Beta-Carotene 13
2.8.1 Distribution 14
2.8.2 Health Benefits of Beta-Carotene 14
2.8.2.1 Anti-cancer 14
2.8.2.2 Skin protection 15
2.8.2.3 Heart health 15
2.8.2.4 Facts about Beta-Carotene 15
CHAPTER 3: MATERIALS AND METHODS
3.1 Collection and Preparation of Samples 16
3.2 Preparation of samples 17
3.3 Analysis of Tomato paste. 17
3.3.1 Determination of phytonutrient 17
3.3.1.1 Determination of Lycopene 17
3.3.1.2 Determination of Phenol 18
3.3.1.3 Determination of flavonoid 19
3.3.1.4 Determination of carotenoids 20
3.3.1.5 Determination of vitamin E 21
3.3.1.6 Determination of vitamin C 22
3.3.1.7 Determination of vitamin A 22
3.3.2 Determination of chemical properties of tomato sample 23
3.3.2.1 Determination of PH 23
3.3.2.2 Determination of titratable acidity 24
3.3.2.3 Determination of Total soluble solids 24
3.3.2.3 Determination of sugar content 25
3.3.3 Determination of mineral content of tomato sample 25
3.3.3.1 Determination of Potassium and sodium 26
3.3.3.2 Determination of Calcium and Magnesium 26
3.3.4 Determination of antioxidative qualities of the selected tomato paste 27
3.3.4.1 Determination of FRAP (Ferric reducing antioxidant potential) 27
3.3.4.2 Determination of the DPPH activity 27
3.3.4.3 Determination of ABTS activity 28
3.7 Statistical analysis 29
CHAPTER 4: RESULTS AND DISCUSSION
4.1: Phytonutrient content of tomato paste 30
4.2: Chemical properties of tomato paste 33
4.3: Mineral content of tomato paste 35
4.4: Antioxidant activity of tomato paste 37
CHAPTER 5: CONCLUSION AND RECOMMENDATIONS
5.1: Conclusion 39
5.2: Recommendation 40
5.3: References 41
LIST OF TABLES
3.1 Identity of selected tomato paste 16
4.1 Phytonutrient content of tomato pastes 32
4.2 Chemical content of tomato pastes 34
4.3 Mineral content of tomato pastes 36
4.4 Antioxidant activity of tomato pastes 38
LIST OF FIGURES
1 A Flavonoid Structure 10
2 A Lycopene Structure 11
3 A Beta-Carotene Structure 14
LIST OF PLATES
1 Sonia tomato paste 17
2 Gento tomato paste 17
3 Super delicieux tomato paste 17
4 Star tomato paste 17
5 Clappa tomato paste 17
CHAPTER ONE
INTRODUCTION
1.1 Background of the Study
Tomato (Lycopersicon esculentum) is an annual vegetable commonly consumed in Nigeria. Over 170 million tonnes of tomato were produced in the world in the year 2014 (FAO, 2014). It is widely a distributed crop which can be grown on several climates. This confers on the crop its ability to survive at various temperatures. More than 30% of global tomato is grown from the Mediterranean (Ahmet and Vedat, 2009).
Tomato is consumed raw, in salads, or after cooking but because they are highly perishable and large wastage is recorded in fresh tomato fruit yearly. Processing operations such as concentration and heat treatment have been applied to ensure their availability all year round (Khachik et al., 2002). Both fresh and processed tomato possesses a high nutritional value, due to its content of vitamins, folates, carotenoids and phenolic compounds (Savatovic-Cetkovic, Canadanovic-Brunet and Dilas, 2010).
Lycopene, the carotenoid which gives tomato its reddish colour, is one of the most powerful scavenger of oxygen singlet and free radicals in body, and plays an important role in many biological functions, such as the modulation of intercellular gap-junction communication, hormonal and immune systems, and metabolic pathways ( Livny et al., 2002; Blum et al., 2005; Basuny et al., 2009).
In addition to carotenoids, tomato contains a variety of natural antioxidants, including ascorbic acid and phenolic compounds (phytochemicals). Dietary intake of tomato and tomato products containing lycopene has been shown to be associated with a decreased risk of chronic diseases such as cancer (breast, colon, prostate) and cardiovascular diseases (Agarwal and Rao, 2000; Willis and Wians. 2003). This implies that commercially sold processed tomato paste should contain a good amount of these phytochemicals. However, adulterated tomato paste, lacking in these important nutrients have entered the market and consequently resulting to economic losses and possibly food poisoning (Ndife et al., 2020). Umeofia, (2016) lamented that the country loses huge sums of money to importation of fake tomato products. He reported that a good number of tomato products imported into the country from Asia were often adulterated with starch which could have adverse effects on the health of Nigerians. Several parameters have been used to assess the quality of tomato paste. These include: the consistency, total solids content, titratable acidity, pH and levels of sugar (Ahmet and Vedat, 2009, Lu et al., 2014). But according to Ndife et al. (2020) the main quality parameters of tomato pastes, as perceived by consumers are colour, consistency and flavour but less emphasis is laid on the nutritional and health benefits derived from consuming the processed tomato. Well- structured studies which examine the quality of different tomato brands sold in Nigeria are limited. Therefore, the aim of this study was to assess the antioxidative and nutritive qualities of selected tomatoes paste sold in Umuahia Metropolis which include the following brands; Sonia, Clappa, Star, Gento and Super delicieux.
1.2 Statement of the problem
The infiltration of poor-quality tomato products into the country has been attributed to the activities of unpatriotic marketers conniving with unethical foreign companies to bring into the country tomato pastes loaded with starch and treated with food colour additives in order to achieve the deep red colour. Therefore evaluating the nutritional quality of tomato products sold in Nigeria will reduce the level of adulteration of such products as they are being envisiaged.
1.3 Objectives of the Study
The major objective of the study was to assess the antioxidative and nutritive qualities of selected tomatoes paste. This is to be achieved through the following specific objectives:
i. To determine the phytonutrient properties of selected tomato paste brands.
ii. To determine the micro nutrient properties of selected tomato paste.
iii. To determine the antioxidative qualities of selected tomato paste.
iv. To determine the physiochemical properties of selected tomato paste
v. To determine the total and reducing sugar of selected tomato paste
1.4 Significance of the Study
With poor regulatory systems and porous borders there is no doubt that it is the responsibility of researchers to generate evidence based data and information which could be helpful in strengthening regulatory agencies, formulate better policies that will benefit the Nation, improve public health and quality of life of citizens. Also, considering the lack of documented information on the assessment of the antioxidative and nutritive qualities of selected tomatoes paste, the information generated would help consumers to make an informed decision when choosing available products.
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