EVALUATION OF CHEMICAL COMPOSITION OF CARROT (DAUCUS CAROTA), SWEET POTATOES (IPOMEA BATATA) AND BEETROOT(BETA VULGARIS)

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ABSTRACT

The proximate, mineral, vitamin and phytochemical contents of orange fleshed sweet     potatoes (O), yellow fleshed sweet potato (Y), Beetroot (B), and carrot (C) were analysed. The samples analysed showed significant differences in their proximate composition, mineral, vitamin and phytochemical contents. The carbohydrate content decreased in the order O (32.37 %) > Y (29.71 %) > B (14.31 %) > C (9.26 %). The crude fibre decreased in the order Y (4.16 %) > B (3.82 %) > O (2.42 %) > C (1.61 %). The Fat content decreased in the order B (4.04 %) > C (2.97 %) > Y (1.54 %) > O (1.06 %). The protein content decreased in the order B (14.85 %) > C (10.51 %) > Y (4.82 %) > O (3.89 %). The ash content decreased in the order B (0.35 %) > C (0.26 %) > Y (0.05 %) > O (0.05 %). The moisture content decreased in the order C (3.54 %) > O (3.41 %) > B (3.21 %) > Y (2.88 %). The samples analyzed are rich sources of minerals (iron, zinc, copper, manganese, phosphorous, nitrogen, sodium, potassium, magnesium calcium and cobalt) that can be used to supplement daily requirements needed for physiological functioning necessary for life. The heavy metals such as nickel (0.01-0.02 ppm), lead (0.02-0.3 ppm) and cadmium (0.00-0.03 ppm) found in the samples were below the permissible limit. Thus, consumption of these samples as foods poses no immediate danger. The samples analyzed have shown to be rich sources of b-carotene (3.25-7.87 mg/100g), vitamin C (17.61-40.71 mg/100g), vitamin A (9.51-18.45 mg/100g), Riboflavin (0.08-0.38 mg/100g), niacin (0.77-2.24 mg/100g) and thiamine (0.11-0.15 mg/100g). Yellow fleshed sweet potato recorded the highest values for vitamin A, C and b-carotene. Carrot recorded the highest values for riboflavin and thiamine. The quantitative phytochemical screening of the samples revealed the presence of alkaloids, phenol, saponin, flavonoids, phytate, and tannin. Beetroot showed highest values for all the phytochemicals analysed. Except for phenol, orange and yellow fleshed potato recorded higher values for all the phytochemicals analysed than carrot. The result of this study showed that the samples analysed contained appreciable amount of essential nutrients and are safe for human consumption.




TABLE OF CONTENTS

Title page                                                                                                                     ii

Certification page                                                                                                        iii

Dedication                                                                                                                   iii

Acknowledgements                                                                                                    iv

Table of Contents                                                                                                        v

List of Tables                                                                                                              vii

List of Figures                                                                                                             ix

List of Plates                                                                                                               x

Abstract                                                                                                                      xi

 

CHAPTER 1: INTRODUCTION                                                                          1

                                                                                            

1.1               Background of the Study                                                                                        

1.2               Statement of Problem                                                                                                 3

1.3               Aim and Objectives                                                                                        4

1.4               Significance of Study                                                                                     4

                                                                                            

CHAPTER 2: REVIEW OF RELATED LITERATURE

                                                                                                        

2.1. Carrot                                                                                                                   5

2.2. Chemical Composition of Carrots                                                                        6

2.3. Health Benefits of Carrots                                                                                   9

2.3.1        Antioxidant, anticarcinogen and immunoenhancer benefits                           9

2.3.2        Anti-diabetic, cholesterol and cardiovascular disease lowering

and anti-hypertensive benefits                                                                       13

2.3.3        Hepatoprotective, and renoprotective benefits                                               14

2.3.4        Wound healing benefits                                                                                  15

2.4. Sweet Potato                                                                                                         16

2.5. Importance of Sweet Potato                                                                                18

2.6. Chemical Composition of Sweet Potato                                                              19

2.7. Nutritional Benefit of Sweet Potato                                                                     20

2.8. Health Benefit of Sweet Potato                                                                           23

2.9. Beetroot                                                                                                               23

2.10. Importance of Beetroot                                                                                      24

2.11. Chemical Composition of Beetroot                                                               24

2.12. Health benefit of Beetroot                                                                             25

CHAPTER 3: MATERIALS AND METHODS

                                                                                

3.1. Sample Collection and Processing                                                                    26

3.2. Proximate Composition                                                                                     26

3.2.1. Determination of moisture                                                                             26

3.2.2. Determination of Ash                                                                                    27

3.2.3. Crude protein determination                                                                          27

3.2.4. Determination of crude fat                                                                            29

3.2.5. Determination of Crude fiber                                                                        29

3.3. Phytochemical determination                                                                            30

3.3.1 Preliminary qualitative analysis                                                                       30           3.3.2 Quantitative Analysis                                                                                  31

3.4. Heavy Metal Determination                                                                              33

3.5. Vitamin Determination                                                                                     35

3.5.1. Determination of ascorbic acid                                                                      35           3.5.2 Determination of riboflavin                                                                         38

3.5.3. Determination of thiamin                                                                               38

3.5.4. Determination of vitamin A and carotenoid.                                                 38

3.6. Statistical Analysis                                                                                             40

        

CHAPTER 4: RESULTS AND DISCUSSION

                                                                                 

4.1. Proximate Composition                                                                                     41

4.2. Mineral Content                                                                                                44

4.3. Vitamin Content                                                                                               50

4.4. Phytochemical Content                                                                                     54

CHAPTER 5: CONCLUSION AND RECOMENDATION

5.1. Conclusion                                                                                                        57

5.2. Recommendation                                                                                              57

       References

       Appendices                                                                                                                   


 

LIST OF TABLES

2.1 Composition of raw carrot (per 100g)                                                                   8

2.2. Nutritive value of sweet potato per 100g to other food source                           22

4.1 Proximate composition of orange fleshed potato, yellow flesh sweet potato,

       Beetroot and carrot                                                                                              41

4.2: Mineral content of orange fleshed potato, yellow flesh sweet potato, beetroot

       and carrot                                                                                                              45

4.3: Vitamin content of orange fleshed potato, yellow flesh sweet potato,

         beetroot and carrot                                                                                              50                  

4.4: Phytochemicals content of orange fleshed potato, yellow flesh sweet potato,

        beetroot   and carrot                                                                                              54

 

 

 

 

 

 

 

 

 

 

 

 

 

 

LIST OF FIGURES

 

2.1       Alpha Carotene                                                                                               9

2.2       Beta Carotene                                                                                                 9

 

 

 

 

 

LIST OF PLATES

2.1       Carrot                                                                                                              6

2.2       Sweet Potatoes                                                                                               18

 

 

 

 

 

 

CHAPTER 1

INTRODUCTION

1.1.   BACKGROUND OF THE STUDY

Over the last decade an abundance of research has shown that common foods and vegetables constitute important functional food components by contributing vitamins, minerals and biologically active compounds which are associated with dietary activities (Kimura and Rodriguez-Amaya, 2003). These foods and vegetables also contain several types of photosynthetic pigments that are chlorophylls and carotenoids (Kimura and Rodriguez-Amaya, 2002). The composition of these pigments produces specific colouration of the food, which is one of the assessed visual quality attributes (Xue and Yang, 2009). In addition, chlorophyll and carotenoid concentration correlate to the photosynthetic potential of plants giving some indication of the physiological status of the plant (Gamon and Surfus, 1999). However, the content of pigments in plants is important, not only due to the colouration and physiological function, but also due to their acknowledged roles in health (Liu et al., 2007).

The name carotenoids is derived from the fact that they constitute the major pigment in the carrot root, (Daucus carota). Carotenoids are undoubtedly among the most widespread and important pigments in living organisms. Carotenoids are the pigments that are responsible for the colours of many plants, fruits and flowers. Carotenoids are fat soluble nutrients and categorized as either xanthophylls or carotenes according to their chemical composition. Carotenoids are found in common foods and vegetables. Most xanthophylls are found in green leafy vegetables and nearly all carotenes are found in yellow vegetables. The most important carotenoids are alpha-carotene, beta-carotene, and beta-cryptoxanthin, lutein, violaxanthin, neoxanthin, and lycopene.  Beta-carotene, alpha-carotene, beta-cryptoxanthin are carotenes that are converted into vitamin A or retinol in the body. They are found in many yellow fruits and vegetables. Beta-carotene is the most widely studied carotenoid. Lutein and zeaxanthin are both stored in the retina of the eye. Neither converts to vitamin A. Both are powerful antioxidants and may be very important for healthy eyes (Wisniewska and Subczynski, 2006).  Lycopene is responsible for the red colour in fruits and vegetables, including tomatoes, red grapes, watermelon, and pink grapefruit. It is also found in papayas and apricots. It does not convert to vitamin A but may have important cancer fighting properties and other health benefits (Sangeetha and Baskaran, 2010).

Currently, interest is being focused on the nutritional and medicinal aspects of different individual carotenoid. Since they are   growing in importance and value, their sources are very important. For example, carotenes are the sources of vitamin A (Olson, 1994). Lutein and zeaxanthin are important factors for human vision (Wisniewska and Subczynski, 2006). Carotenoids have an important role in the prevention of various diseases associated with oxidative stress, such as cancer, cardiovascular diseases and other chronic diseases (Sangeetha and Baskaran, 2010). Humans cannot synthesize the pigments but are able to deposit dietary pigments as absorbed or with slight modification of their structure (Larsen and Christensen, 2005).


1.2.   STATEMENT OF THE PROBLEM

Among succulent vegetable crops, the carrot ranks third in world production (Yamaguchi, 1983). In Nigeria, it is a recent addition to our diet before the mid-50s. But, currently the crop has become a common commodity in the Northern part of the Country, particularly at the end of the dry season - March to May, when large quantities are produced and marketed further south, where little of the crop is grown (Green, 1973, Sarkindiya and Yakubu, 2006). 

Nigeria is said to be the third largest producer of sweet potatoes in the world when it comes to quantity, after China and Uganda. In 2010, about 2.5 % of the world’s production of sweet potatoes was produced in Nigeria (Ahmad et al., 2014). However, sweet potatoes are still not considered a major crop in the country. In the year 2010, sweet potatoes was the tenth highest produced single food crop in Nigeria (after cassava, yam, oil palm fruit, maize, sorghum, millet, paddy rice, and plantains)(Ahmad et al., 2014). In 2010, the total income from agricultural production value for sweet potatoes was $954 million USD and accounted for 1.73 % of total agricultural production value for all crops (Ahmad et al., 2004).  Despite this, sweet potato and carrot have received limited research attention especially as sources of carotenoids and other micronuitrents. The carotenoid profiles of some tropical root and tuber crops have been reported in literature: yams and cocoyam (Champagne et al., 2010), cassava (Ceballos et al., 2012), sweet potato (Ukom et al., 2011).

Appropriately processed root and tuber staples needs to be potential food-based intervention in order to combat micronutrient deficiency since they are fair sources of iron, zinc and vitamin A.


1.3.   AIM AND OBJECTIVES

This study was designed to evaluate the chemical compositions of sweet potato, carrot and beetroot.

The specific objectives are

I.          To determine the proximate composition, phytochemical, mineral and vitamin contents of carrot and sweet potato and beetroots

II.       To compare the chemical composition of sweet potato, beetroot and carrot.


1.4.   SIGNIFICANCE OF THE STUDY

The results of this study can be used as fundamental data for dietary recommendation to help the consumers to select appropriately to meet their nutrient and health needs.


 

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