DETERMINATION OF PHYSIOCHEMICAL AND MICRONUTRIENT COMPOSITION OF SMOOTHIE FROM BLENDS OF PAWPAW, COCONUT AND MILK.

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ABSTRACT

Smoothies are non alcoholic drinks that is mainly produces by mixing fruits without juicing. They are usually made from blends of fruits which is cultivated in different countries including Nigeria. Physiochemical Properties and micronutrient composition of Smoothie from blends of Pawpaw, coconut and milk were investigated. Smoothies were produced from fruit blends (100:0:0), (80:10:10) (70:20:10) (60:30:10) of Pawpaw, Coconut and Milk. Smoothies made with 100℅ of Pawpaw served as Control. Proximate composition, Mineral, Vitamin and Physiochemical Properties as well as the Sensory properties of the smoothies were evaluated using statistical analytical procedures. Data generated were statistically analysed by one way analysis of variance using Statistical product for service solution version 23.0. The Proximate composition revealed that the moisture content (40.75-58.39%) crude protein (0.96-4.34%), fat (0.52-14.02%), Crude fibre (0.04-0.10%), ash (0.30-0.57%), Carbohydrate (22.63-57.44), Energy value (233.94-235.48) of the smoothies. Minerals like Ca (37.10-48.53), Mg (15.72-118.75), K (12.64-23.55) P (22.13-30.10), Na (10.33-58.98), Fe (1.67-2.47), Zn (1.73-1.98) and Physiochemical Properties which are pH (4.70-5.04), %TTA (0.41-0.48) and % TS (41.61-59.29) were also obtained in the smoothies. The smoothies possessed pro vitamin A (116.53-192.24), Vitamin B (0.61-0.71), Vitamin B2(0.95-2.32), Vitamin C (27.00-39.06). The Sensory analysis of the smoothies revealed that the mean scores for appearance ranged from 6.15-7.55 whereas6.35-7.00, 6.10-7.15, 6.40-7.35, 6.85-7.20 was obtained as the mean score for taste, flavour, mouth feel and general acceptability of the smoothies. This study showed that nutrient dense and acceptable smoothies can be produced from fruit blends of Pawpaw Coconut and Milk.







TABLE OF CONTENTS

Title page                                                                                                                                i

Certification                                                                                                                           ii

Dedication                                                                                                                               iii

Acknowledgements                                                                                                                iv

Table of contents                                                                                                                    v

List of tables                                                                                                                           ix

List of figures                                                                                                                         x

List of Plates                                                                                                                            xi

Abstract                                                                                                                                   xii

 

CHAPTER 1: INTRODUCTION 

1.1 Background of the study                                                                                                   1

1.2 Statement of the problems                                                                                                3

1.3 Objectives of the study                                                                                                     5 

1.3.1 General objectives of the study                                                                                     5 

1.3.2 Specific objectives of the study                                                                                     5

1.4 Significance of the study                                                                                                  6 

 

CHAPTER 2: LITERATURE REVIEW

2.1 Fruits                                                                                                                                 8 

2.1.1 Postharvest losses of fruits in Nigeria                                                                           9

2.2 Pawpaw                                                                                                                             10

2.2.1 Nutritional value and health benefits of pawpaw                                                          11 

2.2.2 Processing and food uses of pawpaw                                                                            14

2.3 Coconut                                                                                                                             16

2.3.1 Nutritional and therapeutic benefits of coconut                                                            17

2.3.2 Processing and food uses of coconut                                                                             19

2.4 Overview of milk                                                                                                             22 

2.4.1 Composition of milk                                                                                                      23

2.4.2 Health benefits of milk                                                                                                  25 

2.4.3 Utilization of milk in food products                                                                              26 

2.5 Smoothie                                                                                                                           27

2.5.1 Nutritional and health benefits of smoothie                                                                  28

 

CHAPTER 3: MATERIALS AND METHODS

3.1 Study design                                                                                                                     30

3.2 Sources of raw materials                                                                                                  30

3.3 Sample preparation                                                                                                            30

3.3.1 Processing of smoothie from pawpaw, coconut and milk blends                               30

3.3.2 Formulation of smoothie from paw paw, coconut and milk                                         33

3.4 Methods of analyses                                                                                                         35 

3.5 Proximate analysis                                                                                                            35 

3.5.1 Determination of moisture content                                                                                35  

3.5.2 Determination of ash content                                                                                         35

3.5.3 Determination of fat content                                                                                           36

3.5.4 Determination of crude fibre                                                                                         36 

3.5.5 Determination of crude protein                                                                                     37

3.5.6 Determination of carbohydrate content                                                                         38 

3.5.7 Determination of energy value                                                                                      38 

3.6 Mineral analysis                                                                                                                39

3.6.1 Determination of calcium and magnesium                                                                   39 

3.6.2 Determination of phosphorus                                                                                        40

3.6.3 Determination of potassium                                                                                          40

3.6.4 Determination of sodium                                                                                               41 

3.6.5 Determination of zinc                                                                                                    42

3.6.6 Determination of iron                                                                                                    42

3.7 Vitamin analysis                                                                                                               43

3.7.1 Determination of pro-vitamin a                                                                                     43

3.7.2 Determination of vitamin b1 (thiamin)                                                                          44

3.7.3 Determination of vitamin b2 riboflavin                                                                        45

3.7.4 Determination of vitamin c (ascorbic acid)                                                                   46  

3.8 Physiochemical analysis                                                                                                   47

3.8.1 Determination of ph                                                                                                       47

3.8.2 Determination of titratable acidity                                                                                47

3.8.3 Determination of total solid                                                                                           47

3.9. Sensory evaluation                                                                                                          48

3.10 Statistical analysis                                                                                                          49

 

CHAPTER 4: RESULTS AND DISCUSSION

4.1 Proximate composition of smoothie made from pawpaw, coconut and milk                50

4.2 Mineral content of smoothie made from pawpaw, coconut and milk                            57           

4.3 Vitamin content of smoothie made from pawpaw, coconut and milk                           64

4.4 Physiochemical properties of smoothie made from pawpaw, coconut and milk                      68

4.5 Sensory Properties                                                                                                            72       

CHAPTER 5: CONCLUSION AND RECOMMENDATIONS

5.1 Conclusion                                                                                                                        76   

5.2 Recommendations                                                                                                            77

      References                                                                                                                        78

     

 

 

 

 

 

 

LIST OF TABLES

 

Table 3.1: formulation of pawpaw, coconut and milk (%) for production of smoothie     33 

Table 4.1  proximate composition of smoothie made from pawpaw, coconut and milk        52

Table 4.2: mineral composition of smoothie made from pawpaw, coconut and milk          59       

Table 4.3: vitamin content of smoothie made from pawpaw, coconut and milk                66

Table 4.4: physicochemical properties of smoothie made from pawpaw, coconut and milk        70  

Table 4.5: sensory properties of smoothie made from pawpaw, coconut and milk                      74 

 

 

 

 

 

 

LIST OF PLATES

 

Plate 1: Sample in airtight container                                                                                      34

Plate 2: Sample for sensory evaluation                                                                                  34

Plate 3: Sample for sensory evaluation                                                                                  34

 

 

 

 

 

 

LIST OF FIGURES


Figure 3.1: flow chart for processing smoothie from coconut, pawpaw and milk blends     32

 

 

 


  

 

 

CHAPTER 1

INTRODUCTION 

 

1.1 BACKGROUND OF THE STUDY

Smoothie is a non-alcoholic drink that is mainly produced by blending whole fruits (Castillejo et al., 2015). Although, ingredients like lemonade, yoghurt, milk, condiments, and tea or spices are most times incorporated in it (Castillejo et al., 2015). Smoothie provides fibre, and in turn enhances intake of micronutrients, especially magnesium, iron, potassium, selenium, and vitamin A (Derbyshire, 2017). Therapeutic benefits like antihypertensive, antioxidative, anti-diabetic, anti-inflammatory, and anti-carcinogenic activities are well reported in smoothie (Castillejo et al., 2015; Taiwo, 2018). Blending of various fruits, crops, etc in production of smoothie is a cost-effective means of reducing their underutilization, enhancing the nutritive value of the smoothie, and as well as the organoleptic properties like flavour and taste (Srivastava et al., 2019).

Pawpaw (Carica papaya (L.)) is one of the most powerful nutritional fruits cultivated around the tropical and subtropical regions of the world (Basalingappa et al., 2018). Pawpaw is a rich source of vitamin C and vitamin B. Its mineral content consists of potassium, magnesium, calcium, iron, manganese, phosphorus and zinc (Pooja et al., 2019). Pawpaw is considered as nutraceutical fruit due to its multifaceted medicinal properties. The prominent medicinal properties of pawpaw include anti-fertility, diuretic, uretonic, anti-hypertensive, hypolipidemic, anti-helmintic, wound healing, anti-fungal, antibacterial, antitumor and free radical scavenging activities (Mahendra and Nikhil, 2016). Vitamin C rich foods, such as pawpaw, provide humans with protection against inflammatory poly arthritis, a form of rheumatoid arthritis involving two or more joints. The fiber of pawpaw is able to bind cancercausing toxins in the colon and keep them away from the healthy colon cells (Mehul and Samir, 2016). Pawpaw fruit can be utilized in various ways. It is used in marmalade, puree, wine, nectar, juice, frozen slices, mixed beverages, ice-cream, powder, complementary food, among many others (Saran and Choudhary, 2013).

Coconut is botanically known as Cocos nucifera. It is a member of the family Arecaceae (palm family) (Raj and Magesh, 2015). Coconut is classified as a “functional food” because it provides many health benefits beyond its nutritional content (Poonam, 2013). Coconut contains crude protein (9.22 %), crude fibre (27.84 %), lipid (23.64 %), carbohydrate (35.36 %), and minerals like magnesium, calcium and phosphorus (Imo et al., 2018). Lima et al. (2015) reported that coconut possess analgesic, antiarthritic, antibacterial, antipyretic, antihelminthic, antidiarrheal, and hypoglycemic activities. In addition, other properties such as antihypertensive, anti-inflammatory, antimicrobial, antioxidant, cardioprotective, antiseizure, cytotoxicity, hepatoprotective, vasodilation, nephroprotective, and anti-osteoporosis effects were also reported in coconut (Lima et al., 2015). The demand for coconut is high because it can be utilized in production of a wide array of food products (Sangamithra et al., 2013).

Milk is a whitish liquid produced by the mammary glands of all adult female mammals (Guetouache et al., 2014) or from certain plant materials. Milk is a widely consumed beverage that is essential to the diet of several millions of people worldwide (Visioli and Strata, 2014). Milk is considered as a wholesome complete food providing macro (fat, proteins, and carbohydrates) and micronutrients (calcium, selenium, riboflavin, vitamin B12, and pantothenic acid vitamin B) (Paul et al., 2019) which can significantly contribute towards the overall growth and maintenance of the body system (Mäkinen et al., 2016). Milk is mainly a good source of calcium (Ca) and phosphorus (P), which are necessary for bone growth, development, metabolism, and maintenance (Robinson et al., 2013).


1.2 STATEMENT OF THE PROBLEMS 

Food security has become an issue of global concern in the recent time. Nigeria, with her huge endowed natural and human resources is not spared. Nigerian food crisis is a product of colonial disorientation that has led to neglect of food crops sub-sector as well as over reliance on cash crops production and the oil sector (Attah, 2012). In developing nations like Nigeria, postharvest loss is one of the concerns of food security. Up to 50 % of fruits are loss in these countries (Aysel et al., 2019). Moisture content, respiratory rate, ethylene production, endogenous plant hormones and exogenous factors like microbial growth, temperature, relative humidity, and atmospheric conditions are few of the numerous factors that contributes to postharvest losses of these fruits (Yahaya and Mardiyya, 2019). These factors contributes greatly in mitigating food security. 

 

 Despite the nutritional and health benefits of pawpaw, the postharvest loss of the fruit is higher due to its perishable nature. The fruit has a limited shelf life of less than a week under ambient tropical conditions. This contribute in escalating food insecurity in developing nations like Nigeria. 

 

Fruits like pawpaw are seasonal thus, depriving consumers from ingesting them at certain months of the year and as well obtaining its immense nutritional value. Furthermore, smoothies being mainly produced with fruits makes it lack variety in terms of quality attributes like taste, flavour etc. 

 

Micronutrient malnutrition induces disorders that are most common in economically deprived developing countries. This is as a result of frequent consumption of foods that are deficient in minerals and vitamins. Micronutrient malnutrition has many adverse effects on human health, not all of which are clinically evident.  

Carbonated beverages are consumed by the general populace in Nigeria especially among the youths. The high rate in consumption of carbonated drinks can be attributed to the growing population, urbanization, lack of portable water and climate change (hot weather) among others. Carbonated drinks are composed of high amount of sugar, calories, caffeine and provide inadequate nutritional value. Excessive consumption of these carbonated drinks, however, has negative effect on the body. The aforementioned reasons prompted carrying out of this research.


1.3 OBJECTIVES OF THE STUDY 

1.3.1 General objectives of the study 

The general objectives of the study was to determine the physiochemical and micronutrient composition of smoothie made from blends of pawpaw, coconut and milk.

 

1.3.2 Specific objectives of the study

The Specific objectives are to:

i. produce smoothie from blends of pawpaw, coconut and milk

 ii. determine the physiochemical content (pH, total titratable acidity and total solid) of smoothie

 iii. determine the proximate composition (protein, moisture, fat, crude fibre, ash, carbohydrate and energy value) of smoothie

 iv. determine the mineral content (zinc, iron, calcium, phosphorus, magnesium, sodium and potassium) of smoothie

v.              evaluate the vitamin content (Pro vitamin A, vitamin B1, B2 and C) of smoothie

vi.            evaluate the sensory properties (appearance, flavour, taste, mouth-feel and general acceptability) of the smoothie. 

1.4 SIGNIFICANCE OF THE STUDY 

Considering micronutrient malnutrition which is prevalent in developing countries like Nigeria, processing of smoothie from blends of pawpaw, coconut and milk will contribute to its eradication among the populace. Akinyele and Akinkunmi (2012) revealed that fruits provide the body with important micronutrients, in the right proportion for human growth and development. 

The develop smoothie will go a long way in minimizing postharvest losses of pawpaw fruits to both farmers and consumers thereby enhancing its utilization in food system, and as well as making it available even in it’s off season. 

Incorporation of coconut and milk in the smoothie will aid in providing a novel variety of smoothie to the populace thereby enhancing the nutritional value of the food product, particularly its micronutrient to the general public. 

Individuals who cherish fruits will find this smoothie highly valuable due to its enhanced nutritional value. 

The processed smoothie will go a long way in enhancing food security in developing countries like Nigeria and in turn aid in reducing consumption of carbonated drinks. 

Considering the presence of milk in the smoothie, individuals who are deficient in calcium will find the smoothie to be highly beneficial. 

Data obtained in this study will be of immense benefit to smoothie processing outlets, and researchers.  

 

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